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InvisibleADoobie
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stems into h202 soak for karo clone
    #3710587 - 02/01/05 12:02 AM (12 years, 28 days ago)

Hey there...just making sure I am following my research properly;

If I were to (all things being done in a glove-box):

Cut a clean bit of stem from mushroom. Drop into h202 solution, let soak for 1 hour. Remove. Drop stem into PC'd Karo jar. Incubate.

I would come up with some healthy karo right?

'Doobie


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ADoobie said:
I know what strikes me as odd about this. It is totally unnatural. The whole idea is just totally backwards.

So are historians going to look back at 'The Great Cake Eaters'? Damn. That was a backwards step in the evolutionary process. We left Shroomheads in the dust. Welcome to the new age of Vermheads.

VvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVv

I ONLY STUDY legal, edible mushrooms and mycology. Any mention of any other kind of mushroom is purely fictional, and pictures are ripped from the internet. I do not grow mushrooms!


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Invisiblemetasin
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Re: stems into h202 soak for karo clone [Re: ADoobie]
    #3710704 - 02/01/05 12:24 AM (12 years, 28 days ago)

you should split the mushie without using a scalpel while its still moist, use a scalpel to remove a piece of the inside portion of it to throw in there. You don't want to use a knife to split it cause it can spread any contams if they are there. If its from the inside of the mushie, you shouldnt have to worry about a h202 bath since it hasn't come into contact with any bad shit, and since youre doing this in a glove box or in front of a flow hood you shouldn't have any worries.


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InvisibleADoobie
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Re: stems into h202 soak for karo clone [Re: metasin]
    #3710748 - 02/01/05 12:35 AM (12 years, 28 days ago)

Would the same hold true for something bought from a store? Like could you do the same with Shiitakes, or would they require a bath?

Since that is more for the gourmet boards, address this;

what if your mushie was the LAST one you had, your gills were dirty so you didn't want to make a sporeprint and you were just PUSHED to make a clone from a not-so-perfectly-clean stem.

Now how does that h202 bath look?

Thanks for the reply btw. I will go ahead and do that...but I am still lacking the knowledge


--------------------
ADoobie said:
I know what strikes me as odd about this. It is totally unnatural. The whole idea is just totally backwards.

So are historians going to look back at 'The Great Cake Eaters'? Damn. That was a backwards step in the evolutionary process. We left Shroomheads in the dust. Welcome to the new age of Vermheads.

VvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVv

I ONLY STUDY legal, edible mushrooms and mycology. Any mention of any other kind of mushroom is purely fictional, and pictures are ripped from the internet. I do not grow mushrooms!


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Invisiblemetasin
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Re: stems into h202 soak for karo clone [Re: ADoobie]
    #3710770 - 02/01/05 12:40 AM (12 years, 28 days ago)

I think youre missing what I'm saying
If you split the mushie open, the inside should be contam free unless there is something that is contamed on the outside that fell on it when you split it. If you question the sterility of what you are doing, and it is the last of what you got for now, split it and make a few jars from a few pieces of tissue. Your chances of furthering your progress will be so much better if you do a few in case one contams. Just the odds, ya know??


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Invisiblemetasin
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Re: stems into h202 soak for karo clone [Re: metasin]
    #3710776 - 02/01/05 12:41 AM (12 years, 28 days ago)

an h202 bath in the right percentage is never a bad thing IMHO, but if done correctly to get a clone you shouldn't have to worry about it.


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InvisibleADoobie
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Re: stems into h202 soak for karo clone [Re: metasin]
    #3710839 - 02/01/05 12:56 AM (12 years, 28 days ago)

That makes sense. I got what you are saying. Thanks for the thoughts.

'Doobie


--------------------
ADoobie said:
I know what strikes me as odd about this. It is totally unnatural. The whole idea is just totally backwards.

So are historians going to look back at 'The Great Cake Eaters'? Damn. That was a backwards step in the evolutionary process. We left Shroomheads in the dust. Welcome to the new age of Vermheads.

VvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVv

I ONLY STUDY legal, edible mushrooms and mycology. Any mention of any other kind of mushroom is purely fictional, and pictures are ripped from the internet. I do not grow mushrooms!


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDirtMcgirt
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Re: stems into h202 soak for karo clone [Re: ADoobie]
    #3710935 - 02/01/05 01:15 AM (12 years, 28 days ago)

Quote:

Just the odds, ya know??


:thumbup:

especially when doing tissue clones.  They contam way more often than a spore liquid culture.  Better do a few.


--------------------
"And we, inhabitants of the great coral of the Cosmos, believe the atom (which still we cannot see) to be full matter, whereas, it too, like everything else, is but an embroidery of voids in the Void, and we give the name of being, dense and even eternal, to that dance of inconsistencies, that infinite extension that is identified with absolute Nothingness and that spins from its own non-being the illusion of everything."


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InvisibleADoobie
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Re: stems into h202 soak for karo clone [Re: DirtMcgirt]
    #3710949 - 02/01/05 01:18 AM (12 years, 28 days ago)

Whenever working with agar or liquid cultures, I always do 5 samples anyhows...plus it gives me more objects to love, admire, and inspect from every angle :smile:


--------------------
ADoobie said:
I know what strikes me as odd about this. It is totally unnatural. The whole idea is just totally backwards.

So are historians going to look back at 'The Great Cake Eaters'? Damn. That was a backwards step in the evolutionary process. We left Shroomheads in the dust. Welcome to the new age of Vermheads.

VvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVvVv

I ONLY STUDY legal, edible mushrooms and mycology. Any mention of any other kind of mushroom is purely fictional, and pictures are ripped from the internet. I do not grow mushrooms!


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: ½ Pint Jars, Agar, Laminar Flow Hood

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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