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OfflinePlaCoFuGostei
Brazilian Hunter

Registered: 01/07/05
Posts: 63
Loc: Brazil
Last seen: 11 years, 6 months
My Sterile Micro Environment
    #3701911 - 01/30/05 12:36 PM (11 years, 10 months ago)

OK... let us explain what it is all about.

The system is all too easy.

The flame keeps the air surrounding it without contamination.

Besides it creates an air flow that throws all the contaminant agents at a towel soaked with bleach, (a 3% chlorine/water solution, Clorox).

So between the arrows the environment is sterile.

Just this.



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:mushroom2::thumbup: Peace and Prosperity :thumbup::mushroom2:


Edited by PlaCoFuGostei (01/31/05 04:35 AM)


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Invisibleblissedout
Male User Gallery

Registered: 11/11/04
Posts: 22,320
Loc: Yonder Flag
Re: My Sterile Micro Environment [Re: PlaCoFuGostei]
    #3702251 - 01/30/05 02:56 PM (11 years, 10 months ago)

Cool. Are those jets? What part of Brazil are you from? I have a very good friend from Sao Paulo. She lives here, now. I also loved the movie 'City of God'. Very disturbing, but good. Nice set-up and good luck!!


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:murray:


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OfflinePlaCoFuGostei
Brazilian Hunter

Registered: 01/07/05
Posts: 63
Loc: Brazil
Last seen: 11 years, 6 months
Re: My Sterile Micro Environment [Re: blissedout]
    #3702366 - 01/30/05 03:33 PM (11 years, 10 months ago)

Hey blissedout !!! We are from S?o Paulo too.

Thanks !!!


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:mushroom2::thumbup: Peace and Prosperity :thumbup::mushroom2:


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OfflineKGB920
KGB

Registered: 01/23/05
Posts: 131
Loc: New York City
Last seen: 11 years, 4 months
Re: My Sterile Micro Environment [Re: PlaCoFuGostei]
    #3702381 - 01/30/05 03:40 PM (11 years, 10 months ago)

isnt it a hazard having a flame so close to a bleach soaked towel? considering bleach is flamable


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OfflinePlaCoFuGostei
Brazilian Hunter

Registered: 01/07/05
Posts: 63
Loc: Brazil
Last seen: 11 years, 6 months
Re: My Sterile Micro Environment [Re: KGB920]
    #3702403 - 01/30/05 03:46 PM (11 years, 10 months ago)

I am talking about Clorox !!!!

A 3% chlorine / water solution.


--------------------
:mushroom2::thumbup: Peace and Prosperity :thumbup::mushroom2:


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Offlinescooterpop
Foolish Mortal

Registered: 01/08/05
Posts: 56
Loc: 3rd Stone from the Sun
Last seen: 10 years, 8 months
Re: My Sterile Micro Environment [Re: PlaCoFuGostei]
    #3703593 - 01/30/05 08:32 PM (11 years, 10 months ago)

by far the wildest sterilization technique i've seen yet


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Offline4hodmt
AspiringMycologist

Registered: 04/06/04
Posts: 759
Last seen: 2 years, 6 months
Re: My Sterile Micro Environment [Re: scooterpop]
    #3704090 - 01/30/05 10:03 PM (11 years, 10 months ago)

i dont get it. what do those mini airplanes do? that basically seems like, in essence, a lighter for u to work next to? build a campfire, lol. seriously though, im sure other ppl will be confused.. u should specify details better


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Invisiblemetasin
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Registered: 10/12/04
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Re: My Sterile Micro Environment [Re: 4hodmt]
    #3704176 - 01/30/05 10:24 PM (11 years, 10 months ago)

looks like another oven tek. the torch tek :P


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Offlinedistortion
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Re: My Sterile Micro Environment [Re: metasin]
    #3704496 - 01/30/05 11:24 PM (11 years, 10 months ago)

Well, it looks interesting... but I am confused. Care to elaborate a bit on the setup ?


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Think for Yourself. Question Authority.

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OfflineHambo
Limey

Registered: 09/23/04
Posts: 497
Loc: UK
Last seen: 9 years, 9 months
Re: My Sterile Micro Environment [Re: 4hodmt]
    #3704509 - 01/30/05 11:26 PM (11 years, 10 months ago)

So the mini airplanes are drawn on. It's just a torch on a towel.
I mean, theres no way a flame could support, well anything, let alone 4 miniature airplanes.

Seems a lot of rigmarole to provide a not to theoretically sound sterile environment. I never bother myself, Just wash hands and spray a bit of dettol about.


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OfflineElahrairah
new-growth
Registered: 01/17/05
Posts: 67
Last seen: 11 years, 2 months
Re: My Sterile Micro Environment [Re: distortion]
    #3704519 - 01/30/05 11:27 PM (11 years, 10 months ago)

just like the oven tek, the torch pushes air currents around keeping falling particulate matter (dust and contams) from falling downward (into your jars)

the jets are drawn-on depictions of the air flow lines. they would arc around as shown if there were a ceiling just above the picture's edge.


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All instances of FOAF in this post have been replaced by I and My for clarity of reading. No relation to the poster is meant or implied.


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OfflinePlaCoFuGostei
Brazilian Hunter

Registered: 01/07/05
Posts: 63
Loc: Brazil
Last seen: 11 years, 6 months
Re: My Sterile Micro Environment [Re: Elahrairah]
    #3706153 - 01/31/05 05:16 AM (11 years, 9 months ago)

OK... the airplanes are just stylish arrow points.

It is there just to symbolize the air flow.

Imagine... the hot air tends to go up, so the cool air around the hot air tends to go down.

So the flame creates a continuous air flow symbolized by the lines with airplanes stylish arrow points.

The downstream air is full of contaminants that will die immediately :laser: in touch with the 3% chlorine solution (Clorox) soaked towel, so the air flow between the lines with the airplanes stylish arrow points become sterile.

Between the lines, near the flame, we have a sterile environment, so we can open jars in there... inoculate... what ever. :thumbup:

I believe it's more comfortable than been crouched on an oven, IMHO.  :rolleyes:


--------------------
:mushroom2::thumbup: Peace and Prosperity :thumbup::mushroom2:


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Invisibleohmatic
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Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: My Sterile Micro Environment [Re: PlaCoFuGostei]
    #3706183 - 01/31/05 05:27 AM (11 years, 9 months ago)

Quote:

PlaCoFuGostei said:
I believe it's more comfortable than been crouched on an oven, IMHO.  :rolleyes:




oven tek proved not to be working and so is this,
sorry but this is bulls*
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlinelepiota
expert infailures
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Registered: 10/27/04
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Re: My Sterile Micro Environment [Re: ohmatic]
    #3706500 - 01/31/05 07:14 AM (11 years, 9 months ago)

flames are said to create a sterile environment for a few inches around them, the towel is probably useless but if this method keeps you contam-free why should you change it?


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Re: My Sterile Micro Environment [Re: Elahrairah]
    #3706642 - 01/31/05 07:52 AM (11 years, 9 months ago)

Quote:

Elahrairah said:
just like the oven tek, the torch pushes air currents around keeping falling particulate matter (dust and contams) from falling downward (into your jars)





Anno dispelled they myth of the oven tek. The torch tek will be the same


heat will rise but it wont immediately fall creating currents as are depicted
here, what will happen is it will pull air from the lower area around the torch
head, that will in turn pull contaminants with it, heavier particles will drop
into your work...



Edited by Prisoner#1 (01/31/05 07:58 AM)


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Invisibleohmatic
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Re: My Sterile Micro Environment [Re: Prisoner#1]
    #3706649 - 01/31/05 07:55 AM (11 years, 9 months ago)

Quote:

Prisoner#1 said:
Quote:

Elahrairah said:
just like the oven tek, the torch pushes air currents around keeping falling particulate matter (dust and contams) from falling downward (into your jars)





Anno dispelled they myth of the oven tek. The torch tek will be the same




read two posts above :wink:
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Re: My Sterile Micro Environment [Re: ohmatic]
    #3706662 - 01/31/05 07:59 AM (11 years, 9 months ago)

I added a little to mine, a reason that it wont work.


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OfflineLaughingJim
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Registered: 09/30/04
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Loc: USA, ( CT )
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Re: My Sterile Micro Environment [Re: PlaCoFuGostei]
    #3706793 - 01/31/05 09:43 AM (11 years, 9 months ago)

So... you are saying that you are drawing in contaminated air... over your work area, but burning it in the flame...

I seem to think that it would work more like this...



The 400F air will quickly rise, until it reaches about 80F, then that air would pass by mold and contamination on your ceiling, fall down as it cools, pass by your contaminated body, then get sucked back to the flame...

If the convection existed like your airplaines illustrate, your flame would go out in seconds, as there would be no more oxygen left to burn. It has to mix with contaminated air that has oxygen.


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OfflineLaughingJim
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Re: My Sterile Micro Environment [Re: LaughingJim]
    #3706798 - 01/31/05 09:45 AM (11 years, 9 months ago)

Besides, you would have to work in the air that is 300F in order to be sterile... Your gloves won't withstand those temps. Nor will your supplies.


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Don't forget to RATE us if you think we are offering GOOD or BAD advice, Thank-you!

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Offlinerubber
organic lover
Registered: 11/21/04
Posts: 69
Loc: Tokyo, Japan
Last seen: 11 years, 8 months
Re: My Sterile Micro Environment [Re: LaughingJim]
    #3706838 - 01/31/05 10:33 AM (11 years, 9 months ago)

This is something like what i usually do for aseptic culture of bacteria and fungi in my lab. Except that we use a spirit lamp... just remember to work near the flame of the spirit lamp and you should be safe from contaminants.


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