Home | Community | Message Board


FreeSpores.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, Petri Dish

Jump to first unread post. Pages: 1 | 2 | Next >  [ show all ]
OfflinexIcy
/<Nabbifyed>\
 User Gallery

Registered: 01/09/05
Posts: 81
Last seen: 9 years, 5 months
Agaricus in karo?
    #3691048 - 01/27/05 11:27 PM (11 years, 10 months ago)

How well will Agaricus grow in karo?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletahoe
Noob Slayer
Male User Gallery

Registered: 11/27/03
Posts: 6,213
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Agaricus in karo? [Re: xIcy]
    #3691429 - 01/28/05 12:15 AM (11 years, 10 months ago)

i am trying to germinate ports in karo, its been a week and stil nothing. So i dont know.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinexIcy
/<Nabbifyed>\
 User Gallery

Registered: 01/09/05
Posts: 81
Last seen: 9 years, 5 months
Re: Agaricus in karo? [Re: tahoe]
    #3691452 - 01/28/05 12:18 AM (11 years, 10 months ago)

I have an Agaricus in karo since 1/14/05
There is something in there but it could be contam.

I just put some tissue from an Agaricus i found today, I hope these will grow.

-xIcy


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletahoe
Noob Slayer
Male User Gallery

Registered: 11/27/03
Posts: 6,213
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Agaricus in karo? [Re: xIcy]
    #3691485 - 01/28/05 12:24 AM (11 years, 10 months ago)

i had spores germinate on agar but it contamed, i cant find a fresh one to do a tissue transfer.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCiv
Pinning
Male User Gallery

Registered: 10/14/04
Posts: 2,509
Loc: California
Last seen: 1 year, 5 months
Re: Agaricus in karo? [Re: tahoe]
    #3691600 - 01/28/05 12:54 AM (11 years, 10 months ago)

Quote:

i cant find a fresh one to do a tissue transfer.




They're in almost every yard on my street. Though, they we're much more flushed two months ago. If ya want me to go out tommarow and see if I can snag a fresh one, I will :P I had one in my pocket a few months ago - forgot about it for a week or two... ugh...




Are these them? These are younger ones that havn't opened up yet. These grew in my backyard after I moved a chunk of them to a dirt bed in a garden. Shortly after they got obliterated by my dog.


--------------------
"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar


Edited by Civ (01/28/05 01:05 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinexIcy
/<Nabbifyed>\
 User Gallery

Registered: 01/09/05
Posts: 81
Last seen: 9 years, 5 months
Re: Agaricus in karo? [Re: Civ]
    #3691686 - 01/28/05 01:13 AM (11 years, 10 months ago)

That reminds me of this picture of A. xanthodermus: http://mykoweb.com/CAF/species/Agaricus_xanthodermus.html

If it is that, its toxic, and should bruise yellow.

-xIcy


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCiv
Pinning
Male User Gallery

Registered: 10/14/04
Posts: 2,509
Loc: California
Last seen: 1 year, 5 months
Re: Agaricus in karo? [Re: xIcy]
    #3691757 - 01/28/05 01:40 AM (11 years, 10 months ago)

Quote:

Toxic, causes GI upsets for most people although some individuals claim to eat and relish it. The phenolic odor which is intensified by cooking should be a deterrent.





oOoo Yumm. These ones don't bruise yellow at all. In the pic on the two on the right, on the caps, you see white marks, thats where I tried to bruise em when I first found em. Just stayed white, but they match all the other criteria - base of the stripe seems a little dif also.


Edited by Civ (01/28/05 01:42 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinexIcy
/<Nabbifyed>\
 User Gallery

Registered: 01/09/05
Posts: 81
Last seen: 9 years, 5 months
Re: Agaricus in karo? [Re: Civ]
    #3691767 - 01/28/05 01:43 AM (11 years, 10 months ago)

Does it have a "phenolic odor"


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinexIcy
/<Nabbifyed>\
 User Gallery

Registered: 01/09/05
Posts: 81
Last seen: 9 years, 5 months
Re: Agaricus in karo? [Re: xIcy]
    #3691779 - 01/28/05 01:47 AM (11 years, 10 months ago)

Xanthodermus is sometimes confused with this one: http://mykoweb.com/CAF/species/Agaricus_californicus.html

Also poisonous, but it looks less like the one you have.

Quote:

Agaricus californicus is frequently confused with its somewhat larger cousin A. xanthodermus. Both species have caps with brownish discs and pale margins although Agaricus xanthodermus may be entirely white if developing in deep shade. They are best told apart by odor and bruising reactions. Agaricus californicus has a faint, usually barely discernable phenolic odor while fresh specimens of A. xanthodermus usually have a pronounced phenolic odor. Additionally, fresh mataerial of A. xanthodermus bruise bright yellow on the cap margin and stipe base, while in A. californicus the yellow-bruising is faint, if at all. Agaricus californicus can be separated from other Agaricus species that may have a brownish disc, e.g. A. bisporus by its annulus with a thickened "double-lipped" margin.





It could also be this, which is edible: http://mykoweb.com/CAF/species/Agaricus_arvensis.html


-xIcy


Edited by xIcy (01/28/05 01:49 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineShdwstr
FSRCanada
Male

Registered: 02/18/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 2 years, 11 months
Re: Agaricus in karo? [Re: xIcy]
    #3692845 - 01/28/05 11:39 AM (11 years, 10 months ago)

I've tried Ports and White buttons in liquid cultures with dismal success, but worked great on Agar.

Your best bet for a good culture is get some from the supermarket and take a clean tissue section, then break out some petrie dishes.

Enjoy


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineWorkmanV
Psilocybe Microscopist
Male User Gallery

Registered: 03/01/01
Posts: 3,492
Loc: Washington, USA
Last seen: 1 year, 10 months
Trusted Cultivator
Re: Agaricus in karo? [Re: tahoe]
    #3693884 - 01/28/05 04:45 PM (11 years, 10 months ago)

The spores are reluctant to germinate on agar. There is a trick to get them going. You need to put live mycelium on one edge of the plate and spores on the oppostie edge. The metabolic gases from the live mycelium stimulate quick germination. Otherwise you need to heavily load the plates and hope for at least one volunteer spore to go for it to stimulate the rest. It doesn't always happen. Tissue culture from the store is easier.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineShdwstr
FSRCanada
Male

Registered: 02/18/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 2 years, 11 months
Re: Agaricus in karo? [Re: Workman]
    #3694183 - 01/28/05 06:10 PM (11 years, 10 months ago)

That would explain why they won't start in a liquid culture.
The gasses would be dissipated and lost in the liquid medium.

Thanks Workman.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletahoe
Noob Slayer
Male User Gallery

Registered: 11/27/03
Posts: 6,213
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Agaricus in karo? [Re: Workman]
    #3694697 - 01/28/05 07:57 PM (11 years, 10 months ago)

work,
You know who i am right??


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSpeeker

Registered: 02/11/04
Posts: 639
Re: Agaricus in karo? [Re: Workman]
    #3696315 - 01/29/05 03:09 AM (11 years, 10 months ago)

does this trick work cross species?
i.e. can I use another culture to awake those spores?
And if yes, how close relative it must be?
must that culture belong genus Agaricus?

:sun:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinexIcy
/<Nabbifyed>\
 User Gallery

Registered: 01/09/05
Posts: 81
Last seen: 9 years, 5 months
Re: Agaricus in karo? [Re: Speeker]
    #3696350 - 01/29/05 03:19 AM (11 years, 10 months ago)

Wouldnt the 2 species fight then?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 2 years, 8 months
Re: Agaricus in karo? [Re: Workman]
    #3706045 - 01/31/05 04:41 AM (11 years, 9 months ago)

I have tried to germinate spores of different Agaricus species like Agaricus augustus, Agaricus bitorquis, Agaricus arvensis and Agaricus bisporus.
But as you said before - the spores seem to need a special treatment in order to germinate since none of them germinated.
On the internet there where some papers that described this problem a bit and there it says that some of the spores need a cold/heat or a chemical treatment.
Cause I assume that the might need a cold shock (as it occurs in winter naturally) I have frozen some petri dishes with the spores on it the freezer.
If it works I will report back.

Here are links to the papers I have found on the internet:

http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.py.20.090182.001433
http://www.ingentaconnect.com/content/klu/abim/2004/00000040/00000002/00485811
http://www.srs.fs.usda.gov/pubs/ja/ja_wilson015.pdf
http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_abst_e?cjb_b02-009_80_ns_nf_cjb


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSpeeker

Registered: 02/11/04
Posts: 639
Re: Agaricus in karo? [Re: ragadinks]
    #3706813 - 01/31/05 10:04 AM (11 years, 9 months ago)

In this book is mentioned some of those chemicals ..
The Fungi by Michael J. Carlile & Sarah C. Watkinson







:sun:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinexIcy
/<Nabbifyed>\
 User Gallery

Registered: 01/09/05
Posts: 81
Last seen: 9 years, 5 months
Re: Agaricus in karo? [Re: Speeker]
    #3707452 - 01/31/05 02:24 PM (11 years, 9 months ago)

Quote:

Heat shock (20 min at 45?C) and decreased temperature facilitated activation of germination.




Does that mean both are needed or one?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineWorkmanV
Psilocybe Microscopist
Male User Gallery

Registered: 03/01/01
Posts: 3,492
Loc: Washington, USA
Last seen: 1 year, 10 months
Trusted Cultivator
Re: Agaricus in karo? [Re: tahoe]
    #3707540 - 01/31/05 02:50 PM (11 years, 9 months ago)

Yes I do.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineWorkmanV
Psilocybe Microscopist
Male User Gallery

Registered: 03/01/01
Posts: 3,492
Loc: Washington, USA
Last seen: 1 year, 10 months
Trusted Cultivator
Re: Agaricus in karo? [Re: Speeker]
    #3707552 - 01/31/05 02:55 PM (11 years, 9 months ago)

Thats a good question. I have used Agaricus blazii mycelium to stimulate blazii spores with great success. I am now trying Agaricus bitorquis mycelium to stimulate A. blazii spores. I expect that other species might work as well. I have wondered if Psilocybe cubensis would work to encourage Agaricus spores to germinate but I have yet to try it.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2 | Next >  [ show all ]

Amazon Shop for: Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

Similar ThreadsPosterViewsRepliesLast post
* Reishi spores in karo water Cosm 988 2 01/07/05 07:24 AM
by Cosm
* agrocybe aegerita spore germination chrisscole 1,279 4 11/07/06 03:30 AM
by Semilanceata
* Spores, tissue, karo, and gentics. Psilocybeingzz 473 0 09/07/08 05:23 AM
by Psilocybeingzz
* Spore Germination of A.Bisporus *DELETED*
( 1 2 all )
lorbitherize 3,489 21 06/15/08 04:11 PM
by ragadinks
* Store-bought Oyster wont colonise karo ? notsloth 908 2 10/11/04 05:53 PM
by notsloth
* germination of bitorquis chobumms 1,369 13 04/24/09 12:51 PM
by Mycelio
* Portabello spore germination! dstark 1,401 5 04/25/08 04:48 AM
by dstark
* Germination of spores Semilanceata 748 4 04/22/07 09:37 AM
by Semilanceata

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, EvilMushroom666, cronicr, Pastywhyte
2,540 topic views. 2 members, 5 guests and 3 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
High Mountain Compost
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.054 seconds spending 0.001 seconds on 16 queries.