Home | Community | Message Board


The Spore Depot
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Autoclave, HEPA Filter, Impulse Sealer

Jump to first unread post. Pages: 1
InvisibleLouiseLouise
starstruck
Male User Gallery

Registered: 11/02/04
Posts: 3,898
Loc: Searching w/my good eye c...
Can someone set me straight on prints?
    #3687615 - 01/27/05 08:44 AM (11 years, 10 months ago)

I'm almost ready to make some prints, how do I go about this?
Don't the spores need to be absolutly sterile?
If I go to the trade thread, will the print I recieve be sterile?
Is there a way I can sterilize the spores before or while filling the syringe?
I want to use agar's bag tek.

TYIA


--------------------
"That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledobinky
infoseeker
 User Gallery
Registered: 01/30/03
Posts: 2,436
Loc: Wiki, Kentucky
Re: Can someone set me straight on prints? [Re: LouiseLouise]
    #3687624 - 01/27/05 08:55 AM (11 years, 10 months ago)

wipe tuppeware out with rubbing alcholhol, wipe down a piece of alum foil then wipe razor- cut stype as close to cap as possible, drop cap on foil close up shop and check it in 12 hours. Then take it out and fold up print.

Not sure if that is what your looking for.


--------------------
Today?s Pig is Tomorrow?s Bacon


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 11 months, 23 days
Re: Can someone set me straight on prints? [Re: LouiseLouise]
    #3687626 - 01/27/05 08:57 AM (11 years, 10 months ago)

Yes you need sterile spores. You "clean" them by agar cultivation transfering the healthy myc away o new plates till it dont contam anymore.

You want a print tek or a syringe making tek? Since you already mention agars print tek I assume you wanna make a syringe?

I make my syringe like this:
- take a jar where the mushroomcap fits in
- puncture a hole in the lid where your needle fits through
- cover hole with tyvek or stuff with polyfill
- put lid on jar and pc for 15 minutes
- let cool
- take it to your mushrooms, loosen the lid so that you can open it quickly later on
- put a alcohol wiped pin (punaise, nail) in the cap of your choice
- cut the stem as high as possible while you hold the pin.
- quickly transfer it to the jar and place it at the bottom
- just as quick put the lid back on
- let it print 24 hours
- take cap out by the pin, lid back and fast
- sterile syringe (50 CC is good for one print)with sterile water (boil both, or PC) or suck boiling water in syringe and squirt out a few times (in the sink or sumting) then suck in again.
- let cool
- clean the needle, as if you were inoculating, and squirt the water in the jar through the hole
- shake and suck it back (better to scrape the spores from the bottom with a clean scalpel in a clean room and THEN shake)
You could also put non sticky paper or alufoil at the bottom so the spores dont have to be scraped off and you can just shake.

This is how I do it. Making sporeprints is about the same. Take the cap out and store the jar for later use! :laugh: You can also print on alufoil in there (magash tek) and fold the alufoil so your sporeprints take less space. But the more transferring the more contam risks, imho.

Is your question answered?

MF


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineLaughingJim
Anti-Toxin
 User Gallery

Registered: 09/30/04
Posts: 465
Loc: USA, ( CT )
Last seen: 11 years, 2 months
Re: Can someone set me straight on prints? [Re: LouiseLouise]
    #3687633 - 01/27/05 09:01 AM (11 years, 10 months ago)

If you don't have any contamination, then they are OK. Spores from inside the gill of an uncontaminated shroom are as sterile as a new born baby comming from, well, you know...

Do your print in the bag, like agar suggests, and all will be good. I prefer to place my caps over a glass of sterile water, suspended above with sterile toothpicks punched into the cap, so it floats above the water. However, I have also made (Dog cone rings) which are cones like you see dogs wear to stop them from biteing thier stitches. A round piece of paper is cut about the size of a coffee cup. Then in the center you cut a hole about the size of a quarter. Finally a line is cut from the outside to the center so you can tape it into a cone shape by overlapping the cut line after you put it around the shroom, while it is still growing. I also put foil around the stalk where the cone would normally touch the shroom. To limit possible contact contamination from moisture transfer. More complex, but the spore count is higher.


--------------------
Don't forget to RATE us if you think we are offering GOOD or BAD advice, Thank-you!

I participate in the PROTEIN FOLDING PROGRAM helping the shroomery to help others,
FOLDING.STANFORD.EDU
I support www.shroomery.org (Team # 12679)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleLouiseLouise
starstruck
Male User Gallery

Registered: 11/02/04
Posts: 3,898
Loc: Searching w/my good eye c...
Re: Can someone set me straight on prints? [Re: LaughingJim]
    #3687793 - 01/27/05 10:53 AM (11 years, 10 months ago)

K, let me elaborate a little. i want to make prints that i can trade.
Do I just put the caps down on tin foil? Sterile tin foil?
I don't know about the environmental conditions that are required.
I like the idea of using a square of autoclave bag and impulse sealer, so that when they are ready for use, you just inject sterile water to make a syringe, as agar said.
I wanna make sure i send off good prints. Also, is it likely i will recieve good prints?
But, where the caps lie, glove box? how sterile does this process need to be for success?

TY for your input


--------------------
"That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC


Edited by LouiseLouise (01/27/05 10:56 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 191,560
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Can someone set me straight on prints? [Re: LouiseLouise]
    #3687803 - 01/27/05 10:58 AM (11 years, 10 months ago)

the work is best done in a glove box, and sterility is a big issue especialy if
you plan on trading.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Can someone set me straight on prints? [Re: LouiseLouise]
    #3687811 - 01/27/05 11:04 AM (11 years, 10 months ago)

Quote:

LouiseLouise said:
K, let me elaborate a little. i want to make prints that i can trade.
Do I just put the caps down on tin foil? Sterile tin foil?
I don't know about the environmental conditions that are required.
I like the idea of using a square of autoclave bag and impulse sealer, so that when they are ready for use, you just inject sterile water to make a syringe, as agar said.
But, where the caps lie, glove box? how sterile does this process need to be for success?

TY for your input




Yes foil straight from package will do , but if want to be extra careful , just cut into squares fold in half stick in oven @350*F for 1 hr , then store in zip lock bag .
Cut stem as close to cap as possible , place on clean foil in a cabinet or some place where you can minimze air flow , cover with clean jar or coffee cup to further protect print from getting dust etc on it , let sit for min of 12 hrs (should make for nice dark prints)remove cap , recover with cup, let sit for 6 hrs to dry , then fold back in half and double fold edges to seal in . Bingo Bango print ready for stoage or trade . Do not forget to label with species , date , size , darkeness .
Be as clean as humanly possible and the prints will be fine , oh yeah good idea to use gloves the whole time , for more than one reason  :wink:
When you do not have a true lab setting there is always a chance of dirty prints , but if one takes decent measures to protect prints they will be okay , and hell what can folks expect for free ? :wink:
Hope this helps
GL
PS not into the syringe thing so wont even try to guide you there .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSunshinePounder
The Girl withthe Sun in HerEyes

Registered: 11/19/04
Posts: 288
Loc: Peace Man!
Last seen: 7 years, 2 months
Re: Can someone set me straight on prints? [Re: tripndicular]
    #3687930 - 01/27/05 11:54 AM (11 years, 10 months ago)

thanx, I understand.
So trading prints is ify?


--------------------
My [url=www.http://tinyurl.com/6valr target=_blank]Unitarian Jihad Name[/url] is: Sister Claymore of The Short Path
Get yours

If I was the sun, I'd look for the shade~Liberty

Baby I love you, and you love me. Let's love each other to eternity~


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineZeroArmy27
I didn't go to work for a month.
 Arcade Champion: Asteroids 2000

Registered: 09/01/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 1 month
Re: Can someone set me straight on prints? [Re: SunshinePounder]
    #3688298 - 01/27/05 01:46 PM (11 years, 10 months ago)

well, i've not had problems with sterility since i started using my HEPA. I get an extra sterile rubbermaid, put down some heavy tinfoil, and put the caps in there. leave overnight, prints show up.

I've also covered them with a jar before, this works very well. Also printing them to the bottom of a jar works if you're going to make a syringe.

Don't print on crappy paper or light foil. :frown:


--------------------
"a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut

"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemadsickpenguin
newbie
Registered: 08/29/04
Posts: 234
Re: Can someone set me straight on prints? [Re: ZeroArmy27]
    #3688376 - 01/27/05 02:18 PM (11 years, 10 months ago)

Does wally sell heavy duty tinfoil?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Can someone set me straight on prints? [Re: SunshinePounder]
    #3688585 - 01/27/05 03:17 PM (11 years, 10 months ago)

Quote:

SunshinePounder said:
thanx, I understand.
So trading prints is ify?




Not really , most folks tke pretty good care when printing . And if you move up to agar work , even some of the nastiest prints can be utilized . But all in all most prints I have ever gotten from folks were fine for use . But lets be real there are no guarantees  :wink: And most folks are cool enough , that they ill send you another if you had problems . That is what makes this place so great IMO .

SO TRADE AWAY IT IS WHAT KEEPS THE MUSHIES ALIVE !


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Can someone set me straight on prints? [Re: madsickpenguin]
    #3688594 - 01/27/05 03:21 PM (11 years, 10 months ago)

Quote:

madsickpenguin said:
Does wally sell heavy duty tinfoil?




YES just think REYNOLDS brand , not some cheap ass store brand ,is all folks are suggesting . And if you make choice to use the non stick stuff , be prepared to hand make labels , and warn folks how easy it is to spill the print when they go to open it up . The foil refuses even permanent marker , masking tape works best , and the print itself will not stick to the foil , it will flake off as a waffer or in lrg clumps . Should make syringe makers kinda happy , seeing how scraping spores from foil is no issue .
GL


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Autoclave, HEPA Filter, Impulse Sealer

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Storing prints... SleepAid 691 6 03/25/06 05:07 PM
by SleepAid
* weird plastic print card thing??? odizzle 324 4 10/16/07 11:48 PM
by Skeeblix
* spore print sancho 485 3 02/23/03 03:43 PM
by deanofmean
* print making? leanitus 503 5 09/12/07 06:15 PM
by j_db69
* Attempting a sporeprint - a srhoom is almost ready for printing. - edit, were in!!! veda_sticks 810 10 10/20/07 08:31 PM
by veda_sticks
* making a print mephist00 1,674 19 04/26/08 09:36 PM
by mephist00
* Best spore print tek? MycoJunkie 1,724 8 11/15/04 03:44 PM
by beejay
* Making syring by placing whole foil print into jar of sterilized water?
( 1 2 all )
ozzysmygod 3,320 34 01/13/09 02:55 PM
by InTheRainySeason

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
673 topic views. 27 members, 88 guests and 22 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Zamnesia.com
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.061 seconds spending 0.001 seconds on 14 queries.