|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
GNIOM1498
Death Cup
Registered: 12/15/04
Posts: 945
Loc: My home is where my spiri...
Last seen: 3 years, 9 months
|
why not freeze syringe?
#3685892 - 01/26/05 09:38 PM (19 years, 1 month ago) |
|
|
well I found out after much searching that spores are still viable after being frozen in a syringe. So my question is why do they say keep it in the fridge and not the freezer???? Keep it viable a lot longer frozen. Does anyone freeze their syringes and now would this work for honey/water tek mycelium too?
-------------------- ----ALL MY POSTS ARE FICTIONAL-------
Edited by GNIOM1498 (01/26/05 10:48 PM)
|
fresh313
journeyman
Registered: 09/01/03
Posts: 2,537
Last seen: 12 years, 10 months
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3687010 - 01/27/05 12:51 AM (19 years, 1 month ago) |
|
|
they are viable at room temp for years
no need really
putting your honey tek stuff in the fridge is a good idea when youve got enough clouds to make a few 's
|
boxtop703
ThesaurusLinguae Graecae
Registered: 06/26/01
Posts: 5,011
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3687413 - 01/27/05 03:05 AM (19 years, 1 month ago) |
|
|
Freezing would cause the syringe plunger to be expelled thus seperating itself from the barrel.
Not really a good idea for a variety of reasons.
|
ohmatic
searcher
Registered: 02/28/04
Posts: 6,742
Loc: europe
|
Re: why not freeze syringe? [Re: boxtop703]
#3687415 - 01/27/05 03:06 AM (19 years, 1 month ago) |
|
|
also, freezing will most likely destroy most of the spoers since it will literally 'squeeze' them.
also the syringe will leak all the way round since water expands when freezing. peace ohm
-------------------- MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
Re: why not freeze syringe? [Re: ohmatic]
#3687520 - 01/27/05 05:04 AM (19 years, 1 month ago) |
|
|
Hmm...
I am going to do a simple test today, prepare some syringes, freeze some and then test them and see what happens.
|
boxtop703
ThesaurusLinguae Graecae
Registered: 06/26/01
Posts: 5,011
|
Re: why not freeze syringe? [Re: Anno]
#3687560 - 01/27/05 05:35 AM (19 years, 1 month ago) |
|
|
Quote:
Anno said: Hmm... I am going to do a simple test today, prepare some syringes, freeze some and then test them and see what happens.
Not sure if you're testing the spores or the syringe but the syringe results will vary depending on make and fullness (cc's). I would think BD's may stand up the bast.
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
Re: why not freeze syringe? [Re: boxtop703]
#3687569 - 01/27/05 05:56 AM (19 years, 1 month ago) |
|
|
My plan is the following: Fill several syringes with various amounts of the suspension and air and place it into the freezer in various positions. Weight the syringes before and after to measure eventual leaking. Then after 1-2 days in the freezer inoculate PF cakes with it and compare the results with a none frozen syringe of the same batch. I have Braun Omnifix Luer Lock syringes.
|
Holydiver
Stranger
Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea
|
Re: why not freeze syringe? [Re: Anno]
#3687611 - 01/27/05 06:35 AM (19 years, 1 month ago) |
|
|
I have left syringes in the freezer for over a year and they worked fine. Like boxtop said, expect problems with the plunger and leave some space for the water to expand if you can.
There is some mention of spores rupturing in the freezer, but not enough data and obviously it doesn't kill enough of them, as per my experience.
Not alot of freezer testing has been done, but 1) It keeps spores 2) It's not really necessary, as they last at room temp, especially fridge temps, for years anyways!
-------------------- To find a place to live between the negatives and positives.
|
crazyman
Brass or Steel
Registered: 11/06/98
Posts: 932
Loc: chucktown
Last seen: 15 years, 11 months
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3688003 - 01/27/05 10:13 AM (19 years, 1 month ago) |
|
|
Terrence Mckenna often talked about how mushroom spores could have come from other planet in outer space, a hypothesis called Transpermia. He said that certain mushroom spores are so light-weight as to easily be carried out of the atmosphere and into interstellar transit... My point being, that certain mushroom spore can survive extremely low temperatures and radiation,(like in space), for thousands of years, and still remain viable. I'm pretty sure freezing cubensis spores won't harm them. Just watch out for exploding syringes like previously mentioned.
-------------------- I want to live in Northern Exposure. Ed Chigliak would be my pal.
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
Re: why not freeze syringe? [Re: crazyman]
#3688052 - 01/27/05 10:28 AM (19 years, 1 month ago) |
|
|
>My point being, that certain mushroom spore can survive extremely low temperatures and >radiation,(like in space), for thousands of years, and still remain viable.
The situation here is different though. Since the spores in a syringe are hydrated, it could very well be that the ice ruptures the cell walls. According to the above poster this is not the case though, apparently.
|
ohmatic
searcher
Registered: 02/28/04
Posts: 6,742
Loc: europe
|
Re: why not freeze syringe? [Re: Anno]
#3688274 - 01/27/05 11:39 AM (19 years, 1 month ago) |
|
|
uhm dont get me wrong, but after having read fruits of the gods from mckenna, i got the idea that hes a great poet when coming to words - but his outer space mushroom spore theory sounded like crap to be to be honest.. even if they would manage to get to outerspace, re-entering of atmospheres would grill the fuck out of them. yes / no ? peace ohm *edit* and talking of mckenna and mushrooms is like some chapter of its own, since in his eyes, more or less everything relates to them
-------------------- MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
Edited by ohmatic (01/27/05 11:39 AM)
|
Arp
roving mycophagist
Registered: 04/20/98
Posts: 2,191
Loc: in a van by the river
|
Re: why not freeze syringe? [Re: ohmatic]
#3688526 - 01/27/05 12:58 PM (19 years, 1 month ago) |
|
|
Quote:
would grill the fuck out of them. yes / no ?
Maybe they came in a space-capsule with a door featuring a gravity sensing un-lock mechanism?
|
GNIOM1498
Death Cup
Registered: 12/15/04
Posts: 945
Loc: My home is where my spiri...
Last seen: 3 years, 9 months
|
Re: why not freeze syringe? [Re: Arp]
#3690806 - 01/27/05 08:58 PM (19 years, 1 month ago) |
|
|
or a big big big big frozen piece of rock that didn't burn up???? But most ice asteroids or what not are not made of water but Frozen gas so doubt that. some comets on the other hand are made up of some water. There u go a comet hit earth spreading mushrooms all over the place.
-------------------- ----ALL MY POSTS ARE FICTIONAL-------
|
tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3690937 - 01/27/05 09:13 PM (19 years, 1 month ago) |
|
|
Quote:
GNIOM1498 said: why not freeze syringe?
Why not take a science class
|
Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
|
Re: why not freeze syringe? [Re: tahoe]
#3690959 - 01/27/05 09:16 PM (19 years, 1 month ago) |
|
|
why not give us the cliff notes
|
GNIOM1498
Death Cup
Registered: 12/15/04
Posts: 945
Loc: My home is where my spiri...
Last seen: 3 years, 9 months
|
Re: why not freeze syringe? [Re: Prisoner#1]
#3691203 - 01/27/05 09:44 PM (19 years, 1 month ago) |
|
|
I did take a many of science classes...I'm not to sure what u mean by that. Did u know why u shouldn't freeze syringes? It has nothing to do with the spores but with syringe itself obviously has nothing to do with science LOL. That was a pea brain comment. Keep up the good constructive criticism if u can make some that applies to the relevant of what I ask!!!!
-------------------- ----ALL MY POSTS ARE FICTIONAL-------
|
tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3691401 - 01/27/05 10:12 PM (19 years, 1 month ago) |
|
|
spores have water in them when they are in a syringe. Water expands at different rates at different temps. A frozen syringe will cause the spores to to explode. If you could flash freeze it and not give the spores time to explode then you might be okay.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you. My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
Re: why not freeze syringe? [Re: tahoe]
#3692844 - 01/28/05 09:38 AM (19 years, 1 month ago) |
|
|
Freezing water in a syringe and hypothesizing what would happen to whats suspended in the water has nothing to do with science? Wow..
Damn all my ill-informed science teachers talking about gases liquids and solids and the process they go through to change into other forms! Didnt they know that wasnt science? Stupid, stupid teachers!
Thats just..............ah! Thats just.........AH!
|
GNIOM1498
Death Cup
Registered: 12/15/04
Posts: 945
Loc: My home is where my spiri...
Last seen: 3 years, 9 months
|
Re: why not freeze syringe? [Re: scatmanrav]
#3693090 - 01/28/05 10:54 AM (19 years, 1 month ago) |
|
|
Well....freezing spores is fine. What about the people who live in Canada and every spring or whenever has mushrooms growing? Freezing spores are fine if u couldn't freeze spores there would be no mushrooms anywhere in a cold climate. Cell walls exploding? well maybe some but not even close to all. 1 spores syringed i ordered arrived frozen I let it warm up at room temp. That shit grew I don't think u have any idea what u r talking about. My question was why doesn't people recommend freezing them and I found out that the plunger will leak....nothing that one would learn in a science class. I could see my professor lecturing and saying now u don't want to freeze ur psilocybe mushroom syringes because the plunger will leak. LoL....Mycologist my ass.
-------------------- ----ALL MY POSTS ARE FICTIONAL-------
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3693186 - 01/28/05 11:17 AM (19 years, 1 month ago) |
|
|
You didnt learn that water expands when it freezes in science or you didnt learn that plungers will leak if they come out to much in science? Or perhaps you just did shitty in math because you have no problem solving skills and can't put 2 and 2 together? Just some hypothesis'isis...
|
GNIOM1498
Death Cup
Registered: 12/15/04
Posts: 945
Loc: My home is where my spiri...
Last seen: 3 years, 9 months
|
Re: why not freeze syringe? [Re: scatmanrav]
#3693361 - 01/28/05 12:12 PM (19 years, 1 month ago) |
|
|
F&** don't u understand what I asked. SPORES ARE FINE FROZEN I KNOW THIS. Why doesn't the tek recommend freezing them is what I asked. First water shrinks down to 39f then it starts to expand... my plunger worked FINE WHEN I USED IT. PLEASE UNDERSTAND THE NATURE OF MY QUESTION GOD>>>>>>>> THE SCIENCE WAS THAT THE PLUNGER TENDS TO LEAK. THAT'S WHY U DON"T DO IT AHHHHHHH WHERE THE HELL DID U LEARN THE THRESHOLD OF SYRINGES FULL OF FROZEN WATER in a science class?....okay I never learned that. well i guess i could have done some simple calculations took my syringe calculated volume and found out how much the water will expand sounds practical to me eh. Man this is why I hate ppl there are some F*&^ing pricks out on these forums. Do u have to make such stupid ass comments...maybe just hold back next time learn to chill the F*&^ out and don't ride the post with stupid arguments about how about u take a science class because ur an idiot. the nature of these forums (which I thought but i guess I'm wrong) was to help others.
-------------------- ----ALL MY POSTS ARE FICTIONAL-------
|
boxtop703
ThesaurusLinguae Graecae
Registered: 06/26/01
Posts: 5,011
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3693655 - 01/28/05 01:33 PM (19 years, 1 month ago) |
|
|
Quote:
GNIOM1498 said: F&** don't u understand what I asked. SPORES ARE FINE FROZEN I KNOW THIS. Why doesn't the tek recommend freezing them is what I asked. First water shrinks down to 39f then it starts to expand... my plunger worked FINE WHEN I USED IT. PLEASE UNDERSTAND THE NATURE OF MY QUESTION GOD>>>>>>>> THE SCIENCE WAS THAT THE PLUNGER TENDS TO LEAK. THAT'S WHY U DON"T DO IT AHHHHHHH WHERE THE HELL DID U LEARN THE THRESHOLD OF SYRINGES FULL OF FROZEN WATER in a science class?....okay I never learned that. well i guess i could have done some simple calculations took my syringe calculated volume and found out how much the water will expand sounds practical to me eh. Man this is why I hate ppl there are some F*&^ing pricks out on these forums. Do u have to make such stupid ass comments...maybe just hold back next time learn to chill the F*&^ out and don't ride the post with stupid arguments about how about u take a science class because ur an idiot. the nature of these forums (which I thought but i guess I'm wrong) was to help others.
I suggest Valium sir.
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3693664 - 01/28/05 01:34 PM (19 years, 1 month ago) |
|
|
Why couldnt you have just accepted that freezing is not as good as putting them in the fridge?
|
GNIOM1498
Death Cup
Registered: 12/15/04
Posts: 945
Loc: My home is where my spiri...
Last seen: 3 years, 9 months
|
Re: why not freeze syringe? [Re: scatmanrav]
#3696020 - 01/28/05 11:13 PM (19 years, 1 month ago) |
|
|
where did i ever say in my posts that freezeing was better then putting in fridge? I asked a question I never stated anything do u really think before u type?
-------------------- ----ALL MY POSTS ARE FICTIONAL-------
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3698093 - 01/29/05 11:38 AM (19 years, 1 month ago) |
|
|
A quote from you: "So my question is why do they say keep it in the fridge and not the freezer???? Keep it viable a lot longer frozen."
You said it keeps it viable a lot longer frozen so why not do that...viable longer=better=you saying freezing is better then the fridge.
Keeps it viable a lot longer frozen. That is not a question, notice how it ends in a period making it a statment. English mustve been after science...
|
GNIOM1498
Death Cup
Registered: 12/15/04
Posts: 945
Loc: My home is where my spiri...
Last seen: 3 years, 9 months
|
Re: why not freeze syringe? [Re: scatmanrav]
#3703949 - 01/30/05 07:27 PM (19 years, 1 month ago) |
|
|
did i say it was better ur putting words in my mouth. i never once said freezing is better.
-------------------- ----ALL MY POSTS ARE FICTIONAL-------
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3718942 - 02/02/05 12:19 PM (19 years, 1 month ago) |
|
|
I prepared several syringes by scraping some spores in a jar with 100 ml sterilized water and 2 drops jetdry, shaking the jar and then filling the syringes.
1 syringe has been put into a freezer for 2 days. The plunger didn't came out since I sucked in ~1cc less than possible.(10cc instead of 11cc)
Day 3 since inoculation, the 3 bottom left jars are from a NON FROZEN syringe.
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
Re: why not freeze syringe? [Re: Anno]
#3729187 - 02/04/05 06:21 AM (19 years, 1 month ago) |
|
|
Day 5 since inoculation
|
tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
|
Re: why not freeze syringe? [Re: GNIOM1498]
#3729548 - 02/04/05 09:19 AM (19 years, 1 month ago) |
|
|
Freezing for long periods especially, will "possibly' damage the plungers rubber seal , making it where solution or air can pass one way or the other. Remember the old rule that things tend too condense when cooled or frozen (this excludes expansion of fluids) so the rubber seal will shrink and the longer it stays shrunk it will take on any memory of this shrunken state. I have yet to see a doc or medic freeze his stuff , I bet it is due to it can damage the syringe .
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
|
tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
|
Re: why not freeze syringe? [Re: ohmatic]
#3729578 - 02/04/05 09:28 AM (19 years, 1 month ago) |
|
|
Quote:
ohmatic said: uhm dont get me wrong, but after having read fruits of the gods from mckenna, i got the idea that hes a great poet when coming to words - but his outer space mushroom spore theory sounded like crap to be to be honest..
even if they would manage to get to outerspace, re-entering of atmospheres would grill the fuck out of them. yes / no ? peace ohm
*edit* and talking of mckenna and mushrooms is like some chapter of its own, since in his eyes, more or less everything relates to them
NO to small a particle to effected by gravitational forces pulling on them at great speeds which is what truly causes things to burn up on entry of thick atmosphers . Space dust falls to earth daily ... do we see it light a fire . NO it is too small , so theoretically a falling mushroom spore could and would survive the fall itself . It is all about terminal velocity . Check it out do some research on the topic . What falls faster an 8lb bowling ball or a feather ? What are their "terminal velocities"?
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
|
tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
|
Re: why not freeze syringe? [Re: Anno]
#3729607 - 02/04/05 09:37 AM (19 years, 1 month ago) |
|
|
Quote:
Anno said: >My point being, that certain mushroom spore can survive extremely low temperatures and >radiation,(like in space), for thousands of years, and still remain viable.
The situation here is different though. Since the spores in a syringe are hydrated, it could very well be that the ice ruptures the cell walls. According to the above poster this is not the case though, apparently.
Hey ANNO ,how bout viewing a batch under a scope,freeze said batch,then review again under scope to try and determine if and how many were damaged by freezing process.My money says "some" may be damaged but not all, and surley many will still be very viable.I agree with the cell wall thing of some if not many.A dried spore IMHO can and will survive alot more abuse ,that is part of the evolutionary development of all fungus'...to survive extremes especially when the spores can go hybernative so to say.Sorry for some wrong terms here but you know what I am getting at I am sure. GL with the research and keep us posted as to your findings.
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
|
>how bout viewing a batch under a scope,freeze said batch,then review again under scope
Yes, I thought about this. One of these days I have to get me the access to a scope again.
>My money says "some" may be damaged but not all
Your monkey must have seen the jars above
|
tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
|
Re: why not freeze syringe? [Re: Anno]
#3729656 - 02/04/05 09:56 AM (19 years, 1 month ago) |
|
|
"One of these days I have to get me the access to a scope again."
The mighty ANNO does not have a scope handy ... oh my God is the sky gonna start falling ?
"Your monkey must have seen the jars above" My monkey? LOL He is on my back asking for some damn mushies this very moment
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
|
lol, I misread money for monkey.......
|
miiddiesman024
Stranger
Registered: 01/21/05
Posts: 62
Last seen: 18 years, 9 months
|
|
Water becomes less dense when it freezes, so unless your freezer is highpowered, chances are there is zero chance of spores "rupturing".
Water forms hexagons when it freezes, and it has air gaps inbetween the molecules, ever looked at a snowflake???
Anyway that causes it to expand, its become less dense, there for the same mass takes up a larger volume, busting your syringe, or whatever.
Water reaches its maximum density at 3.98 degrees Celcius, and from there it drops, and then eventually with enough cold it recondenses some.
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
|
>Water becomes less dense when it freezes, so unless your freezer is highpowered, >chances are there is zero chance of spores "rupturing".
???
The hydrated spores conatin water ->water(ice) expands ->spores expand, some rupture
|
Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
|
Re: why not freeze syringe? [Re: Anno]
#3729719 - 02/04/05 10:23 AM (19 years, 1 month ago) |
|
|
Quote:
Anno said: >how bout viewing a batch under a scope,freeze said batch,then review again under scope Yes, I thought about this. One of these days I have to get me the access to a scope again.
I can do this very thing today, Anno, what was the duration of the freeze for your syringes
|
Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
|
Re: why not freeze syringe? [Re: Prisoner#1]
#3729731 - 02/04/05 10:27 AM (19 years, 1 month ago) |
|
|
2 days.
-------------------- [quote]KristiMidocean said: Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
|
Quote:
miiddiesman024 said: Water becomes less dense when it freezes, so unless your freezer is highpowered, chances are there is zero chance of spores "rupturing".
You didnt look at Annos jars? The un frozen jars are all doing like 4 times better then the frozen spored jars in the first pic.
In the second I dont know which jars those 4 are..Anno? Looks like two and two?
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
Re: why not freeze syringe? [Re: scatmanrav]
#3730099 - 02/04/05 12:03 PM (19 years, 1 month ago) |
|
|
>Looks like two and two?
Yes.
|
metasin
Stranger
Registered: 10/12/04
Posts: 972
|
Re: why not freeze syringe? [Re: Anno]
#3730105 - 02/04/05 12:05 PM (19 years, 1 month ago) |
|
|
I don't understand why you would want to waste a perfectly good syringe by freezing it though.
|
Psychoslut
The Mother Fucking Bear-o-dactyl
Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
|
Re: why not freeze syringe? [Re: metasin]
#3730110 - 02/04/05 12:08 PM (19 years, 1 month ago) |
|
|
For the sake of science is why, its not a waste.
-------------------- [quote]KristiMidocean said: Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
|
metasin
Stranger
Registered: 10/12/04
Posts: 972
|
Re: why not freeze syringe? [Re: Psychoslut]
#3730119 - 02/04/05 12:11 PM (19 years, 1 month ago) |
|
|
heh, as long as its your syringe and not mine :P
|
Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
|
Re: why not freeze syringe? [Re: metasin]
#3730199 - 02/04/05 12:37 PM (19 years, 1 month ago) |
|
|
some folks have a few to spare, I make prints from my harvests and then syringes from that, its really not a waste since the spores were free.
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
Re: why not freeze syringe? [Re: Prisoner#1]
#3730319 - 02/04/05 12:57 PM (19 years, 1 month ago) |
|
|
Hell off 1 quart of rye...3 flushes for a total of at least 100 shrooms..100 prints...even doing like 10 syringes a print you got 1000 syringes. Costs nothing but the time it takes you and that wouldnt even take you so long..a few minutes per syringe..
|
metasin
Stranger
Registered: 10/12/04
Posts: 972
|
Re: why not freeze syringe? [Re: scatmanrav]
#3730441 - 02/04/05 01:14 PM (19 years, 1 month ago) |
|
|
well I haven't ordered syringes yet, all of the ones I have are from ordering spores so they are kinda precious
|
TODAY
Battletoad
Registered: 09/25/03
Posts: 10,218
Loc: Metropolis City, USA
|
Re: why not freeze syringe? [Re: ohmatic]
#3730484 - 02/04/05 01:21 PM (19 years, 1 month ago) |
|
|
Quote:
ohmatic said: also, freezing will most likely destroy most of the spoers since it will literally 'squeeze' them. peace ohm
then how do you explain how spores stay dormant in the freezing winters in some of the regions where they grow naturally?
-------------------- ca'rouse (k-rouz) intr.v. To engage in boisterous, drunken merrymaking.
|
metasin
Stranger
Registered: 10/12/04
Posts: 972
|
Re: why not freeze syringe? [Re: TODAY]
#3730500 - 02/04/05 01:23 PM (19 years, 1 month ago) |
|
|
they are not sitting in water and well hydrated maybe??
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
Re: why not freeze syringe? [Re: metasin]
#3730592 - 02/04/05 01:39 PM (19 years, 1 month ago) |
|
|
For one:
Quote:
recondite said: they are not sitting in water and well hydrated maybe??
For two: As you can tell from Annos post ALL spores arent destroyed..that wasnt the question. Many have used frozen syringes.
The original post claimed that spores were MORE viable for longer when frozen then kept in the fridge so why not freeze them.. They definatly stay viable frozen even in water...but SOME spores will rupture and be no good anymore diminishing the quality of a syringe. THAT was the point of this whole thread.
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
Re: why not freeze syringe? [Re: scatmanrav]
#3739763 - 02/06/05 05:45 AM (19 years, 1 month ago) |
|
|
Day 7 since inoculation.
|
PawPaw
Picktish TexasStyle....
Registered: 12/20/04
Posts: 8,243
Loc: was born down in the sout...
|
Re: why not freeze syringe? [Re: Anno]
#3739843 - 02/06/05 06:38 AM (19 years, 1 month ago) |
|
|
Damn Anno thats very fast ...wowiee
-------------------- Come to Paw Paws place there will be no sleeping tonight Eric just wants to catch reds .. Don?t look back, don?t look back He?s right on your trail Don?t look back, don?t look back He?s just a step away from hell WDYWFM?
|
miiddiesman024
Stranger
Registered: 01/21/05
Posts: 62
Last seen: 18 years, 9 months
|
Re: why not freeze syringe? [Re: PawPaw]
#3740130 - 02/06/05 08:52 AM (19 years, 1 month ago) |
|
|
Um first of all i would like to state that im not someone who is going to take what another person has posted and take it to heart. I will however take physics to my heart. IMO Anno was looking for the results to be conclusive to prove his point, not in the name of science. LOL. Shrooms survive in the wild, and have been surviving in the wild before anyone on this forum, 'super member' or not had anything to do with it. Im not try ing to pick at anyones superiortiy or knowledge of shroom growing, but the member OHM proves that he doesn't know much about science apart from mushrooms. And in all honesty, unless many people repeated the same proces there will be no conclusive evidence shown from annos experiment. He would have to do it ten more times, and then five other people woud have to do it ten more times for any type of result to be conlcusive, one way or the other. THanks for clearing that up for everyone self. LATER>
|
Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
|
|
Quote:
miiddiesman024 said: And in all honesty, unless many people repeated the same proces there will be no conclusive evidence shown from annos experiment.
since Anno doesnt have his microscope available at the moment, I have chosen to duplicate his experiment but to view the microscopic detail as opposed to the Macroscopic
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
|
>IMO Anno was looking for the results to be conclusive to prove his point, not in the name of science.
If you don't mind, what has been my point I wanted to prove?
|
LaughingJim
Anti-Toxin
Registered: 09/30/04
Posts: 465
Loc: USA, ( CT )
Last seen: 18 years, 6 months
|
Re: why not freeze syringe? [Re: Anno] 1
#3740461 - 02/06/05 11:26 AM (19 years, 1 month ago) |
|
|
Ultra-dehydrated spores = longest term storage (Freeze dried, or mild heat dried)
Dried spores = long term storage (Air dried prints)
Hydrated frozen spores = long term storage at the cost of quantity loss (Immediate loss only, depending on how fast/cold they are frozen to)
Hydrated cooled spores = medium term storage with minimal loss (Fridge cooled, loss due to decay of activated spores after long term)
Hydrated warm spores = short term storage with minimal loss (Room temp, loss due to decay of activated spores)
----------
I imagine that they had not mentioned the storage of frozen spores because of the complexity involved with proper freezing methods and the initial loss which some may not find acceptable.
You usually are instructed to make a print, and store the print for better long term results (Years/Months). While refridgerated syringes would be a suggested ideal use that will hold you over until your next batch (Weeks/Months). While the room-temp syringes would be expected to last for a similar period to refridgerated spores (Days/Months).
-------------------- Don't forget to RATE us if you think we are offering GOOD or BAD advice, Thank-you! I participate in the PROTEIN FOLDING PROGRAM helping the shroomery to help others, FOLDING.STANFORD.EDU I support www.shroomery.org (Team # 12679)
|
scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
|
|
Quote:
miiddiesman024 said: Um first of all i would like to state that im not someone who is going to take what another person has posted and take it to heart. I will however take physics to my heart. IMO Anno was looking for the results to be conclusive to prove his point, not in the name of science. LOL. Shrooms survive in the wild, and have been surviving in the wild before anyone on this forum, 'super member' or not had anything to do with it. Im not try ing to pick at anyones superiortiy or knowledge of shroom growing, but the member OHM proves that he doesn't know much about science apart from mushrooms. And in all honesty, unless many people repeated the same proces there will be no conclusive evidence shown from annos experiment. He would have to do it ten more times, and then five other people woud have to do it ten more times for any type of result to be conlcusive, one way or the other. THanks for clearing that up for everyone self. LATER>
Could you please show me a quote where Anno said what would happen? The closest thing I can see is "since the cell walls are filled with water they very wall MAY rupture. This doesnt seem to be the case according to the above poster"
Perhaps Im missing the point he's so desperatly trying to prove?
Well in either case Prisoner will be looking under a microscope...will that be scientific enough for you or is Prisoner also in on the conspriacy?
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
Re: why not freeze syringe? [Re: scatmanrav]
#3750129 - 02/08/05 07:52 AM (19 years, 1 month ago) |
|
|
Day 9 since inoculation:
The jars inoculated with the non frozen syringes are fully visibly colonized.
I will leave the conclusions up to whoever wishes to make any conclusions.
|
Cyber
Ash
Registered: 06/14/04
Posts: 1,476
Loc: Dearborn Michigan
Last seen: 10 months, 6 days
|
Re: why not freeze syringe? [Re: Anno]
#3750161 - 02/08/05 08:07 AM (19 years, 1 month ago) |
|
|
Anno,
How long were the spores in the watter before they were frozen?
The reason I ask is that, We know that when some living things are frozen the water crystals inside the cell rupture the cell walls and make the cell nonviable. If the spore syringe was made with dry spores, then popped in the freezer and frozen. The time from the spores hitting the water to the time when the water was frozen would be just over 1 hour. This short duration would mean that you would have a large quantity of spores that had not hydrated and thus were not damaged in the freezing process. If the syringe was made 24 to 48 hours before being frozen then we can be sure that a good number of hydrated spores survived the freezing process.
|
tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
|
Re: why not freeze syringe? [Re: Anno]
#3750175 - 02/08/05 08:14 AM (19 years, 1 month ago) |
|
|
Quote:
Anno said: >IMO Anno was looking for the results to be conclusive to prove his point, not in the name of science.
If you don't mind, what has been my point I wanted to prove?
I have absolutely no power to jump into ones mind and read it. I can however make a hypothesis , and my hypothesis is ...
ANNO was taking his time to explore his own curiosities and at the same time possibly presenting some evidence that may shed some light on this topic.I in no way believe he was doing this to "prove his point" was the ultimate answer to this concept.From what I have seen ANNO trys to be as scientifically factual as he can be , and when he cant he does all he can to obtain the info.
So with that said ... THANX ANNO for time you took out of your life to help shed some light on the possibility that freezing of spores can be harm full to the overall outcome of such a procedure.THANX ANNO for being the first to step up to the plate and actually do something instead of just providing "lip service" as so many members do from time to time ! If my mind reading services were way off...plz tell me
PS Even if ANNO was doing this to just prove his point ... is that not what the 3 steps of scientific conclusion is all about ? To confirm or deny ones hypothetical view of a topic, possibly turning into scientific fact? hmmmmmmmm puzzling dilemma here , maybe we should do some testing
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
|
Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
|
Re: why not freeze syringe? [Re: Cyber]
#3750200 - 02/08/05 08:25 AM (19 years, 1 month ago) |
|
|
>How long were the spores in the watter before they were frozen?
~24 hours
|
tripndicular
My Minds Eye IsRhizomorphic
Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
|
Re: why not freeze syringe? [Re: Anno]
#3750229 - 02/08/05 08:40 AM (19 years, 1 month ago) |
|
|
My monkey you saw earlier in this post thinks that should be sufficient time to fully hydrate the spores LOL I just saw that,... you are funny ANNO...how often do you see monkies in threads?
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will . So with that said here is our mission statement . Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
|
blackout
Registered: 07/16/00
Posts: 5,266
Last seen: 4 months, 12 days
|
Re: why not freeze syringe? [Re: tripndicular]
#4026837 - 04/07/05 02:58 PM (18 years, 11 months ago) |
|
|
Very good experiment.I always wondered if syringes were still viable once frozen with many claims of it while other claiming no. It is good to see it carried out by a much respected member. I too wonder about full hydration of the spores. If there was a small cluster there may be some in the middle still ok.
I wonder which would still be the most viable in 2 years time, one from the fridge or a frozen one?
I don't grow that much and prefer working with vendor syringes than my own. A frozen syringe is easier to hide for most too, I don't want friends or relatives seeing syringes in my fridge if they happen to look in it, even if they know what they are, and are cool about shrooms the average person is unnerved by the sight of a syringe. Stuck in the back of the deep freeze it a nice discrete place.
Edited by blackout (04/07/05 02:59 PM)
|
|