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Invisiblemetasin
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Re: why not freeze syringe? [Re: Anno]
    #3730105 - 02/04/05 12:05 PM (19 years, 1 month ago)

I don't understand why you would want to waste a perfectly good syringe by freezing it though.

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InvisiblePsychoslut
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Re: why not freeze syringe? [Re: metasin]
    #3730110 - 02/04/05 12:08 PM (19 years, 1 month ago)

For the sake of science is why, its not a waste.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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Invisiblemetasin
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Re: why not freeze syringe? [Re: Psychoslut]
    #3730119 - 02/04/05 12:11 PM (19 years, 1 month ago)

heh, as long as its your syringe and not mine :P

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InvisiblePrisoner#1
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Re: why not freeze syringe? [Re: metasin]
    #3730199 - 02/04/05 12:37 PM (19 years, 1 month ago)

some folks have a few to spare, I make prints from my harvests and then syringes
from that, its really not a waste since the spores were free.

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Offlinescatmanrav
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Re: why not freeze syringe? [Re: Prisoner#1]
    #3730319 - 02/04/05 12:57 PM (19 years, 1 month ago)

Hell off 1 quart of rye...3 flushes for a total of at least 100 shrooms..100 prints...even doing like 10 syringes a print you got 1000 syringes. Costs nothing but the time it takes you and that wouldnt even take you so long..a few minutes per syringe..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
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Casing layer colonization and overlay

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Invisiblemetasin
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Re: why not freeze syringe? [Re: scatmanrav]
    #3730441 - 02/04/05 01:14 PM (19 years, 1 month ago)

well I haven't ordered syringes yet, all of the ones I have are from ordering spores so they are kinda precious

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InvisibleTODAY
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Re: why not freeze syringe? [Re: ohmatic]
    #3730484 - 02/04/05 01:21 PM (19 years, 1 month ago)

Quote:

ohmatic said:
also, freezing will most likely destroy most of the spoers
since it will literally 'squeeze' them.
peace ohm :mushroom2:




then how do you explain how spores stay dormant in the freezing winters in some of the regions where they grow naturally?


--------------------

ca'rouse (k-rouz)
intr.v.
To engage in boisterous, drunken merrymaking.

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Invisiblemetasin
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Re: why not freeze syringe? [Re: TODAY]
    #3730500 - 02/04/05 01:23 PM (19 years, 1 month ago)

they are not sitting in water and well hydrated maybe??

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Offlinescatmanrav
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Re: why not freeze syringe? [Re: metasin]
    #3730592 - 02/04/05 01:39 PM (19 years, 1 month ago)

For one:
Quote:

recondite said:
they are not sitting in water and well hydrated maybe??




For two:
As you can tell from Annos post ALL spores arent destroyed..that wasnt the question. Many have used frozen syringes.

The original post claimed that spores were MORE viable for longer when frozen then kept in the fridge so why not freeze them.. They definatly stay viable frozen even in water...but SOME spores will rupture and be no good anymore diminishing the quality of a syringe. THAT was the point of this whole thread.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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OfflineAnnoA
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Re: why not freeze syringe? [Re: scatmanrav]
    #3739763 - 02/06/05 05:45 AM (19 years, 1 month ago)

Day 7 since inoculation.


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InvisiblePawPaw
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Re: why not freeze syringe? [Re: Anno]
    #3739843 - 02/06/05 06:38 AM (19 years, 1 month ago)

Damn Anno thats very fast ...wowiee  :shocked:


--------------------
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Eric just wants to catch reds ..
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Offlinemiiddiesman024
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Re: why not freeze syringe? [Re: PawPaw]
    #3740130 - 02/06/05 08:52 AM (19 years, 1 month ago)

Um first of all i would like to state that im not someone who is going to take what another person has posted and take it to heart. I will however take physics to my heart. IMO Anno was looking for the results to be conclusive to prove his point, not in the name of science. LOL. Shrooms survive in the wild, and have been surviving in the wild before anyone on this forum, 'super member' or not had anything to do with it. Im not try ing to pick at anyones superiortiy or knowledge of shroom growing, but the member OHM proves that he doesn't know much about science apart from mushrooms. And in all honesty, unless many people repeated the same proces there will be no conclusive evidence shown from annos experiment. He would have to do it ten more times, and then five other people woud have to do it ten more times for any type of result to be conlcusive, one way or the other. THanks for clearing that up for everyone self. LATER>

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InvisiblePrisoner#1
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Re: why not freeze syringe? [Re: miiddiesman024]
    #3740230 - 02/06/05 09:32 AM (19 years, 1 month ago)

Quote:

miiddiesman024 said:
And in all honesty, unless many people repeated the same proces there will be no conclusive evidence shown from annos experiment.




since Anno doesnt have his microscope available at the moment, I have chosen to duplicate his experiment but to view the microscopic detail as opposed to the Macroscopic

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OfflineAnnoA
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Re: why not freeze syringe? [Re: miiddiesman024]
    #3740255 - 02/06/05 09:44 AM (19 years, 1 month ago)

>IMO Anno was looking for the results to be conclusive to prove his point, not in the name of science.

If you don't mind, what has been my point I wanted to prove?

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OfflineLaughingJim
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Re: why not freeze syringe? [Re: Anno] * 1
    #3740461 - 02/06/05 11:26 AM (19 years, 1 month ago)

Ultra-dehydrated spores = longest term storage (Freeze dried, or mild heat dried)

Dried spores = long term storage (Air dried prints)

Hydrated frozen spores = long term storage at the cost of quantity loss (Immediate loss only, depending on how fast/cold they are frozen to)

Hydrated cooled spores = medium term storage with minimal loss (Fridge cooled, loss due to decay of activated spores after long term)

Hydrated warm spores = short term storage with minimal loss (Room temp, loss due to decay of activated spores)

----------

I imagine that they had not mentioned the storage of frozen spores because of the complexity involved with proper freezing methods and the initial loss which some may not find acceptable.

You usually are instructed to make a print, and store the print for better long term results (Years/Months). While refridgerated syringes would be a suggested ideal use that will hold you over until your next batch (Weeks/Months). While the room-temp syringes would be expected to last for a similar period to refridgerated spores (Days/Months).


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Offlinescatmanrav
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Re: why not freeze syringe? [Re: miiddiesman024]
    #3740698 - 02/06/05 01:08 PM (19 years, 1 month ago)

Quote:

miiddiesman024 said:
Um first of all i would like to state that im not someone who is going to take what another person has posted and take it to heart. I will however take physics to my heart. IMO Anno was looking for the results to be conclusive to prove his point, not in the name of science. LOL. Shrooms survive in the wild, and have been surviving in the wild before anyone on this forum, 'super member' or not had anything to do with it. Im not try ing to pick at anyones superiortiy or knowledge of shroom growing, but the member OHM proves that he doesn't know much about science apart from mushrooms. And in all honesty, unless many people repeated the same proces there will be no conclusive evidence shown from annos experiment. He would have to do it ten more times, and then five other people woud have to do it ten more times for any type of result to be conlcusive, one way or the other. THanks for clearing that up for everyone self. LATER>




Could you please show me a quote where Anno said what would happen? The closest thing I can see is "since the cell walls are filled with water they very wall MAY rupture. This doesnt seem to be the case according to the above poster"

Perhaps Im missing the point he's so desperatly trying to prove?

Well in either case Prisoner will be looking under a microscope...will that be scientific enough for you or is Prisoner also in on the conspriacy?


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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OfflineAnnoA
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Re: why not freeze syringe? [Re: scatmanrav]
    #3750129 - 02/08/05 07:52 AM (19 years, 1 month ago)

Day 9 since inoculation:



The jars inoculated with the non frozen syringes are fully visibly colonized.

I will leave the conclusions up to whoever wishes to make any conclusions.

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OfflineCyber
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Re: why not freeze syringe? [Re: Anno]
    #3750161 - 02/08/05 08:07 AM (19 years, 1 month ago)

Anno,

How long were the spores in the watter before they were frozen?

The reason I ask is that, We know that when some living things are frozen the water crystals inside the cell rupture the cell walls and make the cell nonviable. If the spore syringe was made with dry spores, then popped in the freezer and frozen. The time from the spores hitting the water to the time when the water was frozen would be just over 1 hour. This short duration would mean that you would have a large quantity of spores that had not hydrated and thus were not damaged in the freezing process. If the syringe was made 24 to 48 hours before being frozen then we can be sure that a good number of hydrated spores survived the freezing process.

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Invisibletripndicular
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Re: why not freeze syringe? [Re: Anno]
    #3750175 - 02/08/05 08:14 AM (19 years, 1 month ago)

Quote:

Anno said:
>IMO Anno was looking for the results to be conclusive to prove his point, not in the name of science.

If you don't mind, what has been my point I wanted to prove?




I have absolutely no power to jump into ones mind and read it. I can however make a hypothesis , and my hypothesis is ...

ANNO was taking his time to explore his own curiosities and at the same time possibly presenting some evidence that may shed some light on this topic.I in no way believe he was doing this to "prove his point" was the ultimate answer to this concept.From what I have seen ANNO trys to be as scientifically factual as he can be , and when he cant he does all he can to obtain the info.

So with that said ... THANX ANNO  :cool: :thumbup:for time you took out of your life to help shed some light on the possibility that freezing of spores can be harm full to the overall outcome of such a procedure.THANX ANNO :thumbup: :cool: for being the first to step up to the plate and actually do something instead of just providing "lip service" as so many members do from time to time !
If my mind reading services were way off...plz tell me  :wink:

PS Even if ANNO was doing this to just prove his point ... is that not what the 3 steps of scientific conclusion is all about ? To confirm or deny ones hypothetical view of a topic, possibly turning into scientific fact? hmmmmmmmm puzzling dilemma here , maybe we should do some testing :wink:


--------------------
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So with that said here is our mission statement .

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OfflineAnnoA
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Re: why not freeze syringe? [Re: Cyber]
    #3750200 - 02/08/05 08:25 AM (19 years, 1 month ago)

>How long were the spores in the watter before they were frozen?

~24 hours

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