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OfflineGNIOM1498
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why not freeze syringe?
    #3685892 - 01/26/05 11:38 PM (12 years, 2 months ago)

well I found out after much searching that spores are still viable after being frozen in a syringe. So my question is why do they say keep it in the fridge and not the freezer???? Keep it viable a lot longer frozen. Does anyone freeze their syringes and now would this work for honey/water tek mycelium too?


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----ALL MY POSTS ARE FICTIONAL-------


Edited by GNIOM1498 (01/27/05 12:48 AM)


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Offlinefresh313
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Re: why not freeze syringe? [Re: GNIOM1498]
    #3687010 - 01/27/05 02:51 AM (12 years, 2 months ago)

they are viable at room temp for years

no need really

putting your honey tek stuff in the fridge is a good idea when youve got enough clouds to make a few :syringe:'s


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Invisibleboxtop703
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Re: why not freeze syringe? [Re: GNIOM1498]
    #3687413 - 01/27/05 05:05 AM (12 years, 2 months ago)

Freezing would cause the syringe plunger to be expelled thus seperating itself from the barrel.

Not really a good idea for a variety of reasons.


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Invisibleohmatic
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Re: why not freeze syringe? [Re: boxtop703]
    #3687415 - 01/27/05 05:06 AM (12 years, 2 months ago)

also, freezing will most likely destroy most of the spoers
since it will literally 'squeeze' them.

also the syringe will leak all the way round since water expands when freezing.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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OfflineAnnoA
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Re: why not freeze syringe? [Re: ohmatic]
    #3687520 - 01/27/05 07:04 AM (12 years, 2 months ago)

Hmm...

I am going to do a simple test today, prepare some syringes, freeze some and then test them and see what happens.


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Invisibleboxtop703
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Re: why not freeze syringe? [Re: Anno]
    #3687560 - 01/27/05 07:35 AM (12 years, 2 months ago)

Quote:

Anno said:
Hmm...

I am going to do a simple test today, prepare some syringes, freeze some and then test them and see what happens.





Not sure if you're testing the spores or the syringe but the syringe results will vary depending on make and fullness (cc's).

I would think BD's may stand up the bast.


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OfflineAnnoA
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Re: why not freeze syringe? [Re: boxtop703]
    #3687569 - 01/27/05 07:56 AM (12 years, 2 months ago)

My plan is the following:
Fill several syringes with various amounts of the suspension and air and place it into the freezer in various positions. Weight the syringes before and after to measure eventual leaking.

Then after 1-2 days in the freezer inoculate PF cakes with it and compare the results with a none frozen syringe of the same batch.

I have Braun Omnifix Luer Lock syringes.


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InvisibleHolydiver
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Re: why not freeze syringe? [Re: Anno]
    #3687611 - 01/27/05 08:35 AM (12 years, 2 months ago)

I have left syringes in the freezer for over a year and they worked fine. Like boxtop said, expect problems with the plunger and leave some space for the water to expand if you can.

There is some mention of spores rupturing in the freezer, but not enough data and obviously it doesn't kill enough of them, as per my experience.

Not alot of freezer testing has been done, but 1) It keeps spores 2) It's not really necessary, as they last at room temp, especially fridge temps, for years anyways!


--------------------
To find a place to live between the negatives and positives.


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Offlinecrazyman
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Re: why not freeze syringe? [Re: GNIOM1498]
    #3688003 - 01/27/05 12:13 PM (12 years, 2 months ago)

Terrence Mckenna often talked about how mushroom spores could have come from other planet in outer space, a hypothesis called Transpermia. He said that certain mushroom spores are so light-weight as to easily be carried out of the atmosphere and into interstellar transit... My point being, that certain mushroom spore can survive extremely low temperatures and radiation,(like in space), for thousands of years, and still remain viable. I'm pretty sure freezing cubensis spores won't harm them. Just watch out for exploding syringes like previously mentioned.


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I want to live in Northern Exposure. Ed Chigliak would be my pal.



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OfflineAnnoA
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Re: why not freeze syringe? [Re: crazyman]
    #3688052 - 01/27/05 12:28 PM (12 years, 2 months ago)

>My point being, that certain mushroom spore can survive extremely low temperatures and
>radiation,(like in space), for thousands of years, and still remain viable.

The situation here is different though. Since the spores in a syringe are hydrated, it could very well be that the ice ruptures the cell walls. According to the above poster this is not the case though, apparently.


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Invisibleohmatic
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Re: why not freeze syringe? [Re: Anno]
    #3688274 - 01/27/05 01:39 PM (12 years, 2 months ago)

uhm dont get me wrong, but after having read fruits of the gods from mckenna,
i got the idea that hes a great poet when coming to words - but his
outer space mushroom spore theory sounded like crap to be to be honest..

even if they would manage to get to outerspace, re-entering of atmospheres
would grill the fuck out of them. yes / no ?
peace ohm :mushroom2:

*edit*
and talking of mckenna and mushrooms is like some chapter of its own,
since in his eyes, more or less everything relates to them :wink:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (01/27/05 01:39 PM)


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InvisibleArp
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Re: why not freeze syringe? [Re: ohmatic]
    #3688526 - 01/27/05 02:58 PM (12 years, 2 months ago)

Quote:

would grill the fuck out of them. yes / no ?





Maybe they came in a space-capsule with a door featuring a gravity sensing un-lock mechanism? :crazy2:


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OfflineGNIOM1498
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Re: why not freeze syringe? [Re: Arp]
    #3690806 - 01/27/05 10:58 PM (12 years, 2 months ago)

or a big big big big frozen piece of rock that didn't burn up???? But most ice asteroids or what not are not made of water but Frozen gas so doubt that. some comets on the other hand are made up of some water. There u go a comet hit earth spreading mushrooms all over the place.


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----ALL MY POSTS ARE FICTIONAL-------


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Invisibletahoe
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Re: why not freeze syringe? [Re: GNIOM1498]
    #3690937 - 01/27/05 11:13 PM (12 years, 2 months ago)

Quote:

GNIOM1498 said:
why not freeze syringe?



Why not take a science class


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InvisiblePrisoner#1M
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Re: why not freeze syringe? [Re: tahoe]
    #3690959 - 01/27/05 11:16 PM (12 years, 2 months ago)

why not give us the cliff notes


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OfflineGNIOM1498
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Re: why not freeze syringe? [Re: Prisoner#1]
    #3691203 - 01/27/05 11:44 PM (12 years, 2 months ago)

I did take a many of science classes...I'm not to sure what u mean by that.  Did u know why u shouldn't freeze syringes?  It has nothing to do with the spores but with syringe itself obviously has nothing to do with science LOL.  That was a pea brain comment.  Keep up the good constructive criticism if u can make some that applies to the relevant of what I ask!!!! :thumbdown:


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----ALL MY POSTS ARE FICTIONAL-------


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Invisibletahoe
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Re: why not freeze syringe? [Re: GNIOM1498]
    #3691401 - 01/28/05 12:12 AM (12 years, 2 months ago)

spores have water in them when they are in a syringe. Water expands at different rates at different temps. A frozen syringe will cause the spores to to explode. If you could flash freeze it and not give the spores time to explode then you might be okay.


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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Offlinescatmanrav
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Re: why not freeze syringe? [Re: tahoe]
    #3692844 - 01/28/05 11:38 AM (12 years, 2 months ago)

Freezing water in a syringe and hypothesizing what would happen to whats suspended in the water has nothing to do with science? Wow..

Damn all my ill-informed science teachers talking about gases liquids and solids and the process they go through to change into other forms! Didnt they know that wasnt science? Stupid, stupid teachers!

Thats just..............ah! Thats just.........AH!


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineGNIOM1498
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Re: why not freeze syringe? [Re: scatmanrav]
    #3693090 - 01/28/05 12:54 PM (12 years, 2 months ago)

Well....freezing spores is fine. What about the people who live in Canada and every spring or whenever has mushrooms growing? Freezing spores are fine if u couldn't freeze spores there would be no mushrooms anywhere in a cold climate. Cell walls exploding? well maybe some but not even close to all. 1 spores syringed i ordered arrived frozen I let it warm up at room temp. That shit grew I don't think u have any idea what u r talking about. My question was why doesn't people recommend freezing them and I found out that the plunger will leak....nothing that one would learn in a science class. I could see my professor lecturing and saying now u don't want to freeze ur psilocybe mushroom syringes because the plunger will leak. LoL....Mycologist my ass.


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----ALL MY POSTS ARE FICTIONAL-------


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Offlinescatmanrav
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Re: why not freeze syringe? [Re: GNIOM1498]
    #3693186 - 01/28/05 01:17 PM (12 years, 2 months ago)

You didnt learn that water expands when it freezes in science or you didnt learn that plungers will leak if they come out to much in science? Or perhaps you just did shitty in math because you have no problem solving skills and can't put 2 and 2 together? Just some hypothesis'isis...


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
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