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Amazon Shop: ½ Pint Jars, Agar, Petri Dish

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Invisibleohmatic
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blending agar for liquid inoculant
    #3676152 - 01/25/05 05:46 AM (12 years, 4 months ago)

ey!

how much of a standard petri's content would you blend up in sterile water
in order to do a couple of syringes, say two or three?

oh and jeah, say you fix such a syringe and slam it to the fridge with
some air left inside the syringe, could that baby be stored like for
a month or would that be a bad idea?
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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OfflineAnnoA
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Re: blending agar for liquid inoculant [Re: ohmatic]
    #3676153 - 01/25/05 05:50 AM (12 years, 4 months ago)

A wedge.

And, don't slam the syringe against the fridge, it might break :wink:


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Invisibleohmatic
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Re: blending agar for liquid inoculant [Re: Anno]
    #3676156 - 01/25/05 05:52 AM (12 years, 4 months ago)

Quote:

Anno said:
A wedge.

And, don't slam the syringe against the fridge, it might break :wink:




heh allright :wink:
say i 'gently insert it into the fridge' could i keep if for a while
then?

oh jeah, what about a few drops of h2o2 into the sterile water,
cant be bad can it?
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlinejonas
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Re: blending agar for liquid inoculant [Re: ohmatic]
    #3676158 - 01/25/05 05:57 AM (12 years, 4 months ago)

I've heard of people having success with syringes made with living tissue for up to a couple months if kept in the fridge. I'd say a month is safe, but you want to be sure that they won't run out of nutes. Maybe make some honeywater and get that sterile?


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Invisibleohmatic
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Re: blending agar for liquid inoculant [Re: jonas]
    #3676159 - 01/25/05 05:59 AM (12 years, 4 months ago)

so you'd recommend blending them in a VERY light honeywater mix if i wanted
to store them a bit.

wouldnt this addition of nutes, which obvously wouldnt be present in
the just belnded wedge-water, also get them to produce CO2 inside the syringe? --> new problem..
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlinescatmanrav
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Re: blending agar for liquid inoculant [Re: ohmatic]
    #3676179 - 01/25/05 06:19 AM (12 years, 4 months ago)

Yeah...and that is one reason you should NOT have any nutes in it when you store. Things put into storage should not be growing and therefore should not have any nutes. Cultures go into storage once all nutes have been used up and it stops growing.

You can use a few drops of h202 and it will last 2-6 months (maybe more but thats pushing it) stored in the fridge.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineMushroomFriend
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Re: blending agar for liquid inoculant [Re: ohmatic]
    #3676181 - 01/25/05 06:20 AM (12 years, 4 months ago)

the needle has a hole :wink: Maybe contam risk with using that. I think myc syringe can be stored in fridge for a month or 2. Just like one can do with colonized jars or agarplates. Dont know if the being in te water shortens the period but I guess 1 month must be very fine.

They dont need nutes then cause they hybernate c.q. dont grow, so dont need "fuel".

MF


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Invisibleohmatic
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Re: blending agar for liquid inoculant [Re: scatmanrav]
    #3676188 - 01/25/05 06:29 AM (12 years, 4 months ago)

Quote:

scatmanrav said:
Yeah...and that is one reason you should NOT have any nutes in it when you store. Things put into storage should not be growing and therefore should not have any nutes. Cultures go into storage once all nutes have been used up and it stops growing.

You can use a few drops of h202 and it will last 2-6 months (maybe more but thats pushing it) stored in the fridge.



thats ther reason why i talked about plain sterile water from the
beginning.

alrighty :smile: thx for all the input.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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InvisibleRoadkill
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Re: blending agar for liquid inoculant [Re: ohmatic]
    #3677421 - 01/25/05 01:31 PM (12 years, 4 months ago)

Quote:

ohmatic said:
how much of a standard petri's content would you blend up in sterile water
in order to do a couple of syringes, say two or three?

oh and jeah, say you fix such a syringe and slam it to the fridge with
some air left inside the syringe, could that baby be stored like for
a month or would that be a bad idea?
peace ohm :mushroom2:



A wedge..like Anno said.

I have mycelium syringes that have lasted years in the fridge.
:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisibleohmatic
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Re: blending agar for liquid inoculant [Re: Roadkill]
    #3677429 - 01/25/05 01:32 PM (12 years, 4 months ago)

Quote:

Roadkill said:
Quote:

ohmatic said:
how much of a standard petri's content would you blend up in sterile water
in order to do a couple of syringes, say two or three?

oh and jeah, say you fix such a syringe and slam it to the fridge with
some air left inside the syringe, could that baby be stored like for
a month or would that be a bad idea?
peace ohm :mushroom2:



A wedge..like Anno said.

I have mycelium syringes that have lasted years in the fridge.
:smile:




awesome!
lets blend them mofo!

did you leave some air in these syringes?
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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InvisibleRoadkill
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Re: blending agar for liquid inoculant [Re: ohmatic]
    #3677442 - 01/25/05 01:35 PM (12 years, 4 months ago)

Quote:

did you leave some air in these syringes?
peace ohm :mushroom2:




No


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Amazon Shop: ½ Pint Jars, Agar, Petri Dish

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