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Offlinedetaildsn
Dr.Trippinfungus
Registered: 08/05/01
Posts: 20
Last seen: 19 years, 6 months
After innoculation....
    #367436 - 08/05/01 10:28 PM (22 years, 6 months ago)

3 days ago I innoculated 12 jars and placed tape over the holes. They are now in a rubbermaid container in my closet with a heating pad keeping the temp at a constant 85 degrees. I have just a couple of simple questions.
Should the jars lids be kept tightly sealed or loosened up a bit?
Is it better to place jars upside down?


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OfflineMattyB
enthusiast
Registered: 10/12/00
Posts: 238
Last seen: 11 years, 2 months
Re: After innoculation.... [Re: detaildsn]
    #367458 - 08/05/01 11:06 PM (22 years, 6 months ago)

You've got your jars incubating at the perfect temperature. Keep the lids slightly loose (I turn each lid a 1/4 rotation) but remember that a heating pad will speed up evaporation, so a lid that's tighter than normal is desirable in your case. You may need to loosen the lids a little more in case you see a large amount of condensation developing. A small amount is fine, but too much can damage the cake. And you really don't need to flip your jars unless you have problems germinating. Hope this helps.


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Offlinesomebody
Stranger
Registered: 07/11/01
Posts: 7
Loc: Ontario,Canada
Last seen: 20 years, 2 months
Re: After innoculation.... [Re: detaildsn]
    #367463 - 08/05/01 11:17 PM (22 years, 6 months ago)

I'm fairly new at this as well but I've had good results so far. What I have done is keep the jars sealed tighlty until they are about 75% colonized then I loosen the lids to provide a little oxygen. I have done 40 jars (on three seprate occasions) and haven't had any contamination problems so far (*keeping fingers crossed*), and I'm not working in the most sterile conditions either. :)

Good Luck!




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