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Offlinetatter1090
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Registered: 11/13/04
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Copying a spore syringe?
    #3662323 - 01/22/05 12:33 PM (12 years, 6 months ago)

I just received my spore syringe, my question is whether its possible to 'copy' the contents of the syringe as so i do not have to be buying them every once in awhile...I have access to a fully stocked and functioning lab, so if agar or other 'growing' broths would assist me in my endeavours i am set so long as someone can tell me what to do
Thank you


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Offlinelemon_lw
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Re: Copying a spore syringe? [Re: tatter1090]
    #3662337 - 01/22/05 12:36 PM (12 years, 6 months ago)

i just posted this in another post no more than 5 minutes ago. its called karo and it turns 1 cc into 100 cc's
take a pf style jar (one with holes in it). put 2 tsp. of karo in it. boil about 130 cc's of water (allow for the evaporation). once the water is boiling pour it into the jar with the karo in it and place a layer of saran wrap on it real quick(your going to need to have 3 sheets already cut),the heat will kill any initial bacteria, screw the lid on real quick and then place the next two layers of saran wrap on it. place in the fridge to cool down. once cool lift the first layer of saran wrap and inoculate with 1 cc trought the first 2 layers of saran wrap then place the third layer back on. and place in incubator. once ready to inoculate suck it into the syringe shoot back into the karo jar real fast to mix up all the mycelium, and then suck it back into the syringe and into the substrate jar. this will help reduce the chance of contams because you will skip the amount of time it takes to germinate the spores. i have had 100% success with this technique.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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InvisibleRoadkill
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Re: Copying a spore syringe? [Re: tatter1090]
    #3662343 - 01/22/05 12:39 PM (12 years, 6 months ago)

Quote:

tatter1090 said:
I just received my spore syringe, my question is whether its possible to 'copy' the contents of the syringe as so i do not have to be buying them every once in awhile...I have access to a fully stocked and functioning lab, so if agar or other 'growing' broths would assist me in my endeavours i am set so long as someone can tell me what to do
Thank you




You need to do some reading!~

Spore print and syringe making teks...Click here!~

You would grow out your mushrooms and take a spore print.
Then make new syringe's from your print.

Good luck!~


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinetatter1090
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Re: Copying a spore syringe? [Re: Roadkill]
    #3663031 - 01/22/05 04:43 PM (12 years, 6 months ago)

Unless I missed it, none of the tek manuals cover making spore syringes directly from other spore syringes, I could have infered that it would be the same procedure but I just wanted to make sure. So another question, is it possible to take agar plates and inject them with a spore syringe, keep those as a stock (can you freeze them?) and when needed, simply take a slice of the agar plate, and following the syringe making tek's create a syringe? Thanks for your suggestions


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OfflineMycoJunkie
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Re: Copying a spore syringe? [Re: tatter1090]
    #3663038 - 01/22/05 04:44 PM (12 years, 6 months ago)

Well, you could shake it a lot, then squirt half of it into another sterile syringe (after taking out the plunger of course), and then fill each back up with sterile water. Now you have roughly 50% of the spores in each syringe. You'll deliver 50% less spores with each inoculation, but you can inoculate more jars/bags.

Really the best way to attain more spore syringes is by growing the shrooms and then printing them. If this is your first try, I suggest using half of the syringe on 4 or 5 pint jars, 1cc each.


--------------------
:cussing::whip:


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OfflineIGnosticAbhorI
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Re: Copying a spore syringe? [Re: tatter1090]
    #3663042 - 01/22/05 04:45 PM (12 years, 6 months ago)

You can put half of the contents of your syringe in something sterile like a jar, then put that into another syringe. Now fill both up with cool Sterile water...This should be fine. Don't do it again b/c eventually your spore/water ratio will get too fucked up and you'll have way to much water and not much spores..

-Gl

you could always fruit and then make prints....you can make like 30 syringes with a nice flush, probably more

-gnostic


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InvisiblePrisoner#1
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Re: Copying a spore syringe? [Re: tatter1090]
    #3664071 - 01/22/05 09:03 PM (12 years, 6 months ago)

Quote:

tatter1090 said:
Unless I missed it, none of the tek manuals cover making spore syringes directly from other spore syringes




you arent making it from a syringe, you are doing it from a print.

http://www.shroomery.org/index/par/3329


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OfflineMushroomFriend
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Re: Copying a spore syringe? [Re: tatter1090]
    #3666678 - 01/23/05 05:53 AM (12 years, 6 months ago)

The reason they dont mention making a syringe from a syringe is obvious. Depending on the amount of spores in the syringe you could maybe make 2 or 3 more out of them, but spores dont multiply in syringes, they fruit as a mushroom and give spores where you can make new syringes with.......


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