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OfflineSirTripAlot
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To much temp?
    #3657485 - 01/21/05 08:03 AM (12 years, 4 months ago)

Unfortunately had forgotten to turn off my heating pad, which is located under my box, which contains my grow bag. For about 14 hours the bag was subject to temps well over 85 degrees. Will this kill any spores or mycelium?(the bag was more than a little warm when i touched it). Am OK or just a paranoid newbie?????


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“I must not fear.
Fear is the mind-killer.
Fear is the little-death that brings total obliteration.
I will face my fear. I will permit it to pass over me and through me.
And when it has gone past I will turn the inner eye to see its path.
Where the fear has gone there will be nothing.
Only I will remain.”


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InvisibleLand_Crab
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Re: To much temp? [Re: SirTripAlot]
    #3657495 - 01/21/05 08:18 AM (12 years, 4 months ago)

Thermal death of mycelium occurs at 106 degrees F. I don't know what temperature spores can withstand. If the heating pad is not in direct contact with the bag, then it is more likely that you will be O.K., but by no means guaranteed.

Since you mentioned spores, this means that you injected them recently and they have not yet begun to colonize..??


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OfflineLaughingJim
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Re: To much temp? [Re: Land_Crab]
    #3657633 - 01/21/05 10:02 AM (12 years, 4 months ago)

One or two layers of corrigated cardboard between your heat pad, and the jars will ensure that this does not happen again. That will allow the heat to act as it's own insulator while the temps are high. The hotter these things get, the less they create heat.

If you realy have a hard time controlling heat while you are away... it would be worth the investment to get a thermostat of some kind, or switch to a device with less wattage, which will never excede a temprature of that level.

EG, a 5W heat source can raise the temp in a 40 gallon tank to no more than about 5F from the surrounding temp... if it is 75F in your house, the highest temp that the 5W heater will make is 80F in a 40 gallon glass tank. The max temprature of saturation is about 85F, at which point it will not phisically create any more heat. (Shorting out, or self regulating... Due to max amp draw being reached.)

Respectively in an uninsulated incubation chamber (R1+),
(Watts)@(Gallons)=(Increase F) (Sample)
(5W)@(1Gal)=(+10F)(70F to 85F)
(10W)@(2Gal)=(+10F)(70F to 85F)
(25W)@(6Gal)=(+10F)(70F to 85F)
(50W)@(12Gal)=(+10F)(70F to 85F)
(100W)@(25Gal)=(+10F)(70F to 85F)
(200W)@(50Gal)=(+10F)(70F to 85F)

Respectively in an insulated incubation chamber (R10+),
(Watts)@(Gallons)=(Increase F) (Sample)
(5W)@(5Gal)=(+10F)(70F to 85F)
(10W)@(10Gal)=(+10F)(70F to 85F)
(25W)@(25Gal)=(+10F)(70F to 85F)
(50W)@(50Gal)=(+10F)(70F to 85F)
(100W)@(100Gal)=(+10F)(70F to 85F)
(200W)@(200Gal)=(+10F)(70F to 85F)

(Based on general wattage BTU, normalized aproximate temps.)
(Resources taken from multiple manufactures product chooser descriptions, user experiance, BTU mathmatical calculations, and trial and error.)

A standard plastic container TIT, is about R4-R6. A standard plastic container is R1-R2, A standard fish-tank is R0-R1, A standard 3/4 inch plywood container is R5-R7, Foamular 2 inch thick is R10.


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OfflineSirTripAlot
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Re: To much temp? [Re: Land_Crab]
    #3657644 - 01/21/05 10:05 AM (12 years, 4 months ago)

Inoculation occurred 12 days ago.........little signs of mycelium growth observed...........so if my temp was 100 degrees direct contact to heating pad........and 94 degrees indirect contact to my bag..........would I be OK or are they dead??????


--------------------
“I must not fear.
Fear is the mind-killer.
Fear is the little-death that brings total obliteration.
I will face my fear. I will permit it to pass over me and through me.
And when it has gone past I will turn the inner eye to see its path.
Where the fear has gone there will be nothing.
Only I will remain.”


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OfflineLaughingJim
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Re: To much temp? [Re: LaughingJim]
    #3657647 - 01/21/05 10:07 AM (12 years, 4 months ago)

1 (cubic foot) = 7.48051945 US gallons
10 US gallon = 1.33680556 (cubic foot)

1 US gallon = 3.7854118 liter
10 liter = 2.64172051 US gallons


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OfflineLaughingJim
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Re: To much temp? [Re: SirTripAlot]
    #3657651 - 01/21/05 10:09 AM (12 years, 4 months ago)

I say that they have a strong chance of survival. (However, watch to see that the extra heat did not remove too much moisture.)


--------------------
Don't forget to RATE us if you think we are offering GOOD or BAD advice, Thank-you!

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OfflineSirTripAlot
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Re: To much temp? [Re: SirTripAlot]
    #3657652 - 01/21/05 10:09 AM (12 years, 4 months ago)

There was a one layer of cardboard between the heatpad and my grow bag


--------------------
“I must not fear.
Fear is the mind-killer.
Fear is the little-death that brings total obliteration.
I will face my fear. I will permit it to pass over me and through me.
And when it has gone past I will turn the inner eye to see its path.
Where the fear has gone there will be nothing.
Only I will remain.”


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OfflineSirTripAlot
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Registered: 01/11/05
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Re: To much temp? [Re: SirTripAlot]
    #3657657 - 01/21/05 10:11 AM (12 years, 4 months ago)

If moisture is removed from my bag........can i replace it by injecting distilled water through the inoculation site?


--------------------
“I must not fear.
Fear is the mind-killer.
Fear is the little-death that brings total obliteration.
I will face my fear. I will permit it to pass over me and through me.
And when it has gone past I will turn the inner eye to see its path.
Where the fear has gone there will be nothing.
Only I will remain.”


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Invisibletripndicular
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Re: To much temp? [Re: SirTripAlot]
    #3657678 - 01/21/05 10:21 AM (12 years, 4 months ago)

Quote:

SirTripAlot said:
If moisture is removed from my bag........can i replace it by injecting distilled water through the inoculation site?




If you try this you better make damn sure you use "sterilized" distilled water , adding water after inoc during colonization can be very risky IMHO , let her ride .In a sealed enviro , like a bag it is not likely to have dried up enough for you to worry .
As long as temps in the bag did not exceed 104*F you should be okay as far as myc surviving .
That is one of the many probs with using a heating pad IMO , keeping temp range consistant !
GL

edit~ and if you chose to risk injecting water , do it in very small amounts at a time over the course of a few days , if you get to crazy you deffinetly risk inviting contams , that is #1 casue of a lot of contams IMHO too much water content .


--------------------
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Edited by tripndicular (01/21/05 10:23 AM)


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InvisibleLouiseLouise
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Re: To much temp? [Re: SirTripAlot]
    #3657685 - 01/21/05 10:23 AM (12 years, 4 months ago)

You are wasting your time here.
They have a poll that you need to be involved in somewhere.

:peace:


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Amazon Shop: ½ Pint Jars

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