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OfflineRootedGrowth
Grain Apprentice
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Registered: 10/30/04
Posts: 225
Last seen: 19 years, 3 months
Re: Darkened grain and jars not colonizing :( (pics) [Re: derx]
    #3646630 - 01/18/05 11:46 PM (20 years, 2 days ago)

Oh damn, didnt know that derx. That might be what the problem is then....I auto released the steam, rather than just letting the PC cool down. That might be what screwed me.


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Offlinedonkey_lipz
journeyman
Registered: 07/27/04
Posts: 173
Last seen: 12 years, 8 months
Re: Darkened grain and jars not colonizing :( (pics) [Re: RootedGrowth]
    #3646954 - 01/19/05 01:10 AM (20 years, 1 day ago)

It could also have something to with your inoculation techniques.  Did you sterilize the bejesus out of the room before you shot them up?  Make sure you do that.  Perhaps run a hepa for an hour, then shoot them up in the up flow of the hepa.  Flame sterilize the needles, did in alcohol.  Did you make the spore syringes yourself?  Is your tyvek seal fucked up?

It could be a number of things.  I wish you luck in the future!  Dont let the contams get you down, its how ya learn :smile:

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Offlinegsmith
reformed lurker
Registered: 12/07/04
Posts: 204
Last seen: 19 years, 1 month
Re: Darkened grain and jars not colonizing :( (pics) [Re: donkey_lipz]
    #3647294 - 01/19/05 07:13 AM (20 years, 1 day ago)

did you soak the grain over night before you simmered & PC'ed it? That's usually important.


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