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OfflineFlashman
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Registered: 01/05/05
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Multispore Mycology question
    #3640244 - 01/17/05 07:42 PM (11 years, 10 months ago)

Say you have inoculated a jar of rye grain and you had two regions of seperate growth, eg



(Photo 'borrowed' from http://mycotopia.net/discus/messages/2/181155.html?1105965162
)
Assuming the two regions of mycelium growth started from two different sets of spores:

What happens when they meet?

Do they grow along side each other, but as 2 diferent colonies of mycelium?

Or do they 'join' and share nutrient, water etc? (Like when one plant species is grafted onto another)

If they do 'join', do they also share DNA? Is this how strains are created? (in the wild or the lab)

If so, will a fully colonised jar contain one mycelial growth, with its DNA made up from from the variations present in the different myc colonies in the jar?

For example, if the left mcyelium was from a B+ syringe, and the right myc from a Cambodian syringe.

Would you grow B+ and Cambo sbrooms or B+ crossed Cambo hybrid, or all three?

Hope that I'm making sense to somebody.
Thanks


--------------------
Woof Woof


Edited by Flashman (01/17/05 08:05 PM)


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Offlinehyphae
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Re: Multispore Mycology question [Re: Flashman]
    #3640654 - 01/17/05 09:14 PM (11 years, 10 months ago)

They join clamp connections if they are the same strain. Otherwise they will compete for substrate and nutes.


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InvisibleYidakiMan
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Re: Multispore Mycology question [Re: Flashman]
    #3641698 - 01/18/05 01:22 AM (11 years, 10 months ago)

Quote:

Flashman said:
What happens when they meet?



Sexual reporduction in fungi consists of three fundamental events, plasmogamy, karyogamy and meiosis. When two compatible hyphae meet, they undergo plasmogamy. This is when nuclei come together in the same cytoplasm. This is not nuclear fusion or karyogamy. After plasmogamy the mushroom mycelia contains two sets of DNA.

Quote:


Do they grow along side each other, but as 2 diferent colonies of mycelium?



If they are not sexually compatible, then they will remain as two seperate organisms.
Quote:


If they do 'join', do they also share DNA?



Not immediately. Karyogamy is not done until the fungi is ready to make spores.

Quote:

Is this how strains are created? (in the wild or the lab)



Strains are created by selective breeding.



Quote:

If so, will a fully colonised jar contain one mycelial growth, with its DNA made up from from the variations present in the different myc colonies in the jar?



It could be one organism or many.
Quote:


Would you grow B+ and Cambo sbrooms or B+ crossed Cambo hybrid, or all three?





You'd most likely end up with a cambo side and a B+ side. If you wanted a B+ x Cambo cross your only guarenteed bet is to use agar and verify monokaryotic cultures to put into a breeding program.


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OfflineFlashman
Sir

Registered: 01/05/05
Posts: 38
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Last seen: 11 years, 9 months
Re: Multispore Mycology question [Re: YidakiMan]
    #3642271 - 01/18/05 07:04 AM (11 years, 10 months ago)

Thank you both for your replies - this has been bugging me for a while...


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Woof Woof


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