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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

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Offlineinetd
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Mycelium grows in karo solution, but won't grow in BRF/Verm
    #3637809 - 01/17/05 07:02 AM (12 years, 2 months ago)

Hi all, I'm hoping someone could help me out with this problem, or has run into a similar situation.

I've used B+ syringes to inject into a karo solution, and every time I've done so (even with different syringes) mycelium grows fast. So the spores are fine.

The problem is, for the past month or two I've been trying to get the PF-Tek going. And no matter what I try, there's never any growth. Not a bit.

The jars are cleaned very well.
The area is cleaned, as are all tools.
I use brown rice flour within a day or two of buying it from the store. I've experimented with grinding it extremely fine (almost powder,) fine, and fine mixed with more coarse grains.
I've used both filtered water and tap water.
I've experimented with varying levels of water in the substrate, from pretty dry, to really wet.
Varied how compact the mixture has been in the jar also, from really light and airy, to compacted down.

Everything else is pretty standard, sterilyzing everything to the best of my ability, boiling for over an hour and letting everything cool for 12-24 hours, etc.

Some jars I injected with a small bit of spores from the syringes, in others I've put in lots extra.
The last batch I made up some jars and used the mycelium that was floating around in the karo solution to inject. Again, some jars got lots of extra solution. Syringes shaken up.

Incubating, temps never went over 80F. Usually around 74ish.

My first few batches I tried using the ratios from the normal teks I've seen, and other people's suggestions.
When that didn't work is when I started experimenting with varying everything else.

It's not so much that I'm getting desperate, as it's ANNOYING THE HELL out of me. These should be growing!

I'm using Alltreat Farms Premium Vermiculte, and Sunbrown calrose brown rice. GMO Free.
If any of that makes a difference.

I'm at my wits end, and would love to hear some feedback/suggestions from you guys.

Thanks in advance.


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OfflinekronnyQ
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Re: Mycelium grows in karo solution, but won't grow in BRF/Verm [Re: inetd]
    #3637860 - 01/17/05 08:02 AM (12 years, 2 months ago)

Since it's your first time you might wanna just try innoc'ing straight with spores. Sounds like maybe your karo is messed up maybe carmelized.


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OfflineShdwstr
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Re: Mycelium grows in karo solution, but won't grow in BRF/Verm [Re: inetd]
    #3638135 - 01/17/05 11:05 AM (12 years, 2 months ago)

>>> "Incubating, temps never went over 80F. Usually around 74ish."

You might want to kick those temps up to 86F. 74 is too low and slow to incubate at... good for fruiting though.  :cool:


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Offlinedjred
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Re: Mycelium grows in karo solution, but won't grow in BRF/Verm [Re: kronnyQ]
    #3638136 - 01/17/05 11:05 AM (12 years, 2 months ago)

man i agree try spores.also when injecting karo you need to make sure not injecting shit load of karo solution so try and suck up the myc then spit out the water aceess until it reaches tot he point of myc coming out..then your not going to have so much solution to water down your pf cakes


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Invisibleniteowl
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Re: Mycelium grows in karo solution, but won't grow in BRF/Verm [Re: inetd]
    #3638207 - 01/17/05 11:39 AM (12 years, 2 months ago)

Your temps are WAY too low like Shdwstr said. Bump them up 10 degrees.


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Offlineinetd
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Re: Mycelium grows in karo solution, but won't grow in BRF/Verm [Re: niteowl]
    #3639168 - 01/17/05 03:31 PM (12 years, 2 months ago)

The first few jars I did do with spores, but when that didn't work I switched to injecting with karo.

I'll try getting rid of extra water from my syringe with the karo solution, use spores on some other jars, and bump my temps up.

Thanks guys.


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Offlinenewgrower1
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Re: Mycelium grows in karo solution, but won't grow in BRF/Verm [Re: inetd]
    #14207482 - 03/30/11 03:37 AM (5 years, 11 months ago)

86 is to high ask any trusted cultivator what the temps should be.  I am a noob so you dont have to believe me but 74 should be good maybe bump it a few degrees but dont go over 80 you will have less results then none at 86


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Offlinefaceyneck
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Re: Mycelium grows in karo solution, but won't grow in BRF/Verm [Re: newgrower1]
    #14207527 - 03/30/11 04:00 AM (5 years, 11 months ago)

Quote:

newgrower1 said:
86 is to high ask any trusted cultivator what the temps should be.  I am a noob so you dont have to believe me but 74 should be good maybe bump it a few degrees but dont go over 80 you will have less results then none at 86




If I haven't already rated you, I will now. :super:

That is most definitely correct. 86 is crazy high for cubensis colonization.

Room temperature is ideal.

One thing I would've expected at least SOMEONE to ask, though:

How long are you waiting before you call it a failure? :justdontknow:

I've had cakes that take over a month to colonize before. Also, when I start plates, it's not uncommon for me to be waiting over 2 weeks with a friggin' LC before I see white, fuzzy growth.


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Offlineto high
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Re: Mycelium grows in karo solution, but won't grow in BRF/Verm [Re: faceyneck]
    #16258645 - 05/20/12 04:48 PM (4 years, 10 months ago)

i have ben having a similar problem using a honey solution i keep shooting up jars and nothing i am running out of mycelium any thoughts


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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