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Amazon Shop for: ½ Pint Jars, Agar

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InvisibleGnuBobo
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Difference between agar and Karo
    #3637586 - 01/17/05 03:22 AM (11 years, 10 months ago)

    Hi all.  I've been looking at starting up some reishi and perhaps some king oyster and from what I understand, both of these can be used with the karo tek (that's why I've posted in here, as it's a basic question). 
    As I've been reading on the web about using liquid innoculant and agar, I saw some pics (I think on this site called "caps and stems") of an agar mix in the bottom of half  pint jars and it looked semi-liquid in its consistency. 
    I haven't had a great amount of luck with karo yet, for some reason, and was wondering, if you made up your agar in a half-pint jar, and then inoculated that with spores, could you then PC a larger syringe (like, say, 50cc) and then inject what water was left from the PCing in the syringe into the agar jar that has myc in it, then swirl this around and suck it up and have a liquid innoculant that was grown out on a semi-solid agar? 
      I've never used agar, so I'm sorry if this question is really dumb.  I'm just going off of the pictures I've seen, and trying to get a decent amount of liquid innoculant to knock up several jars with reishi since it doesn't flush heavily (I'm looking for quantity of blocks to grow out). 
      Thanks for the help!  :grin:

GB


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Offlinescatmanrav
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Re: Difference between agar and Karo [Re: GnuBobo]
    #3638912 - 01/17/05 02:38 PM (11 years, 10 months ago)

Liquid innoculant is basically 2 things. 1) water and 2) food. Agar is 3 things 1) water 2) food and 3) agar-agar. Agar agar is like a gelitin type product added to solidify the water and food so that you can cut wedges from agar. Agar will start to solidify at temps below like 125 IIRC.

You could make some agar plates and sqirt in aditional water once colonized then suck up the water as you scrape the surface of the agar with the needle. You'll have to be very sterile and quick about it though and if your having problems getting a karo jar to germinate I'm not sure how much easier this method will be.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleGnuBobo
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Re: Difference between agar and Karo [Re: scatmanrav]
    #3639210 - 01/17/05 03:38 PM (11 years, 10 months ago)

Thanks.  I didn't understand the composition of agar.  More reading fer me!  :grin:


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Amazon Shop for: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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