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OfflineCenebite
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Registered: 10/27/04
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How often do you shake WBS?
    #3636470 - 01/16/05 08:32 PM (20 years, 4 days ago)

How often do you guys shake your WBS jars during colonization? What are the effects of this? does it slow growth? speed it up? give a more even growth? I shook after inoculation and now I have some strong clumps forming... should I shake..?

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Invisibleagar
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Re: How often do you shake WBS? [Re: Cenebite]
    #3636486 - 01/16/05 08:33 PM (20 years, 4 days ago)

ONCE @ around 15% colonization. Any more will SLOW process...IMHO


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OfflineCenebite
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Re: How often do you shake WBS? [Re: agar]
    #3636501 - 01/16/05 08:37 PM (20 years, 4 days ago)

alright, I just decided to shake them all haha most were at at least 10%.... I've noticed WBS is taking forever to colonize, its been a full week and not much has colonized... anyones input? I guess they are twice the size of the old 1/2 pint jars I was using.. not really taking that into account..

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InvisibleRoadkillM
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Re: How often do you shake WBS? [Re: Cenebite]
    #3636517 - 01/16/05 08:40 PM (20 years, 4 days ago)

You might have a bacterial contam if they haven't started back up again in a weeks time.

Wait a few more days...if nothing happens...open one jar and see if you smell a bad odor that doesn't smell like mushrooms...that will tell all.

Good luck!~


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.


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OfflineCenebite
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Registered: 10/27/04
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Re: How often do you shake WBS? [Re: Roadkill]
    #3636550 - 01/16/05 08:46 PM (20 years, 4 days ago)

No I dont think its a contam at all I still see growth some jars just more then others... my dark dark home made GT syringes.. those jars are going fast, the purhcased B+ syringes where you couldnt see any spores are taking much longer... plus my incubation temps are rather low..

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Invisibledog
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Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: How often do you shake WBS? [Re: Cenebite]
    #3636597 - 01/16/05 08:54 PM (20 years, 4 days ago)

I shake once immediately after transfer/inoculation and then leave it alone. As agar said shaking too much can and will slow colonization.


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