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Amazon Shop: Agar, Petri Dish, Pressure Cooker

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Offlineshambler
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Registered: 04/08/04
Posts: 55
Last seen: 6 days, 10 minutes
agar question
    #3636112 - 01/16/05 08:51 PM (12 years, 5 months ago)

all the teks say to mix the agar in one large container and pressure cook it. Is there a reason why you cant mix it up and put it in the petri dishes, then put those in the pressure cooker to sterlize?

thanks


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OfflineHamurabi
the babylonianleader..

Registered: 03/31/02
Posts: 2,416
Loc: Greece
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Re: agar question [Re: shambler]
    #3637584 - 01/17/05 03:21 AM (12 years, 5 months ago)

petri dishes are very small in height and in the PC you must put water int he bottom...
it would be easy to get water in the and screw them up! even if u put them ON something,doing this you will have lots of consedation in them


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Invisibleohmatic
searcher
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Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: agar question [Re: Hamurabi]
    #3637713 - 01/17/05 05:09 AM (12 years, 5 months ago)

what he said.

but after you poured them,
you can stack them on a rack and sterilize them.
if you decide to do so, release pressure very slow since the agar will
boil over if there is a rapid pressuer change - causing the petris
to get terribly messed up.
peace ohm :mushroom2:


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: agar question [Re: shambler]
    #3638312 - 01/17/05 12:16 PM (12 years, 5 months ago)

If you are using "pyrex" petris you can pour into them and then sterilize in the dish , but you must also come up with a way to lift the petris out of the water in using a pc , so some sort of tray is needed to lift them up .
Now if using an AC no sweat perp and burn  :wink: so to say .
GL
PS plastic petris will melt , I forgot to say that , if you did not already know it .


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InvisiblePrisoner#1
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 193,665
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Re: agar question [Re: tripndicular]
    #3638339 - 01/17/05 12:22 PM (12 years, 5 months ago)

Quote:

tripndicular said:
but you must also come up with a way to lift the petris out of the water




they shouldnt be in the water.... its best to sterilize the media and plates at the same time, take your PC to the flowhood and then unpack it into the clean air stream... pour your plates, tape them and allow them to cool

sterilizing them after a pour is a great way to have a big mess to cleam up.


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Amazon Shop: Agar, Petri Dish, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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