Home | Community | Message Board


World Seed Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop for:   eBay Lab Glassware   Amazon ½ Pint Jars, 10cc Syringe, Rye Grain

Jump to first unread post. Pages: 1
OfflineFlashman
Sir

Registered: 01/05/05
Posts: 38
Loc: Location, Loction
Last seen: 11 years, 9 months
Grain 2 Peroxide 2 Peroxidated Honey Water - Will this work?
    #3634653 - 01/16/05 12:50 PM (11 years, 10 months ago)

Will this work? I'm worried about contams making honey water, as I've only got an oven to work over. I'm also short of spores and thought about using colonised grain...

Materials

Colonised rye
A beaker containing between 3 and 7 ml of 3% peroxide per liter of water).
Jar of prepared, sterile honey water with same peroxide concentration as above.

Method

Take a few kernels of colonised rye, and drop in the soloution of peroxide.

Then put the colonised kernels into the jar of peroxidated honey water. Then incubate the honey water.

(Assume working over oven, and sterile technique is used)

Explanation of Method

The idea is the peroxide / water soloution will sterilize / kill any spores that may contaminate the colonised grain during the transfer.

Peroxidated honey water is used to kill any spores that may enter the jar while adding the colonised grain.

I guess the peroxide should be added to the honey water jar after its been PC'd.

Opinions? Criticisms? Improvements?


--------------------
Woof Woof


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: Grain 2 Peroxide 2 Peroxidated Honey Water - Will this work? [Re: Flashman]
    #3634667 - 01/16/05 12:54 PM (11 years, 10 months ago)

if you were to do this, i'd do it as follows:

sterilize a fill with 10cc sterile water and suck up very
few h2o2 in it, so you get a nice 0.3% h2o2 syringe.

inject the content of the syringe into your rye jar, shake around quite
hard and suck up the resulting broth.
youll have nice mycelium in there in a h2o2/water solution.

use this syringe to nock up karo/honeywater.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Edited by ohmatic (01/16/05 01:02 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFlashman
Sir

Registered: 01/05/05
Posts: 38
Loc: Location, Loction
Last seen: 11 years, 9 months
Re: Grain 2 Peroxide 2 Peroxidated Honey Water - Will this work? [Re: ohmatic]
    #3634685 - 01/16/05 01:01 PM (11 years, 10 months ago)

Like the poor man's mycelium syring tek... Cool.

Just to clarify, the first syring contains the 0.3 peroxide soloution, and is used to inject into the colonised rye grain jar.

The second syinge is used to remove the mycelium broth from the rye jar, and add to the honey water jar?

Should I still peroxidate the honey water?

Thanks


--------------------
Woof Woof


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: Grain 2 Peroxide 2 Peroxidated Honey Water - Will this work? [Re: Flashman]
    #3634692 - 01/16/05 01:03 PM (11 years, 10 months ago)

sorry i misstyped, you do it all with one syringe,
i edited my post :wink:
there shouldnt be a need to peroxide the honey water even further
since youll shoot some peroxide in it with the syringe anyway.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFlashman
Sir

Registered: 01/05/05
Posts: 38
Loc: Location, Loction
Last seen: 11 years, 9 months
Re: Grain 2 Peroxide 2 Peroxidated Honey Water - Will this work? [Re: ohmatic]
    #3634773 - 01/16/05 01:41 PM (11 years, 10 months ago)

Using a 0.3% peroxide syringe to inject / collect mycelium broth will collect a random sample of myc cells (cottony & rhizomorphic)

Using a grain to peroxide to peroxidated honey water will allow selection of a grain showing thick & healthy rhizomorphic growth. I guess a lot more myc will be transferred this way, so the honey water will colonise faster.

Is this reason enough to to a Grain => Peroxide => Peroxide Honey water instead of the syringe tek?


--------------------
Woof Woof


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleohmatic
searcher
 User Gallery

Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: Grain 2 Peroxide 2 Peroxidated Honey Water - Will this work? [Re: Flashman]
    #3634781 - 01/16/05 01:44 PM (11 years, 10 months ago)

well i would do as i told you above, b/c this way you eliminate
contam sources and transfer a liquid which holes h2o2.
i'd do it this way since its the most logical for me.
transferring grains should work aswell though.
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop for:   eBay Lab Glassware   Amazon ½ Pint Jars, 10cc Syringe, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Re: peroxide/h2o ratio for casing Anonymous 466 2 04/28/00 10:55 PM
by Anonymous
* Can I add certain % Peroxide to substrate mix? Numba9 4,110 5 08/23/01 11:31 AM
by shellacct
* Grain to Agar? Aiko Aiko 1,474 12 05/23/16 01:00 PM
by bodhisatta
* PEROXIDE??? SAMOAN 977 3 11/22/01 02:08 AM
by Anonymous
* Awesome rye grain h2o2 tek dimitri211 4,555 1 08/17/01 10:28 PM
by jonnyshaggs420
* Sterilzation of grain by soaking? trauma47645 1,122 17 04/10/06 12:32 PM
by FooMan
* grain to grain transfer...gone bad
( 1 2 all )
mikeytro 4,077 28 10/15/07 05:38 PM
by PowRGnome
* Peroxide and LCs Nobitte 656 5 02/21/12 05:56 PM
by Nobitte

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
945 topic views. 30 members, 96 guests and 18 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.041 seconds spending 0.003 seconds on 14 queries.