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Amazon Shop: ½ Pint Jars, Agar

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Offlineinfamous
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inoculation using Agar
    #3633628 - 01/16/05 01:23 AM (12 years, 8 months ago)

Hey when my dishes of agar are complete and im ready to incubate my jars how do I go about doing this?? Should I make a solution out of the mycelium, or could i place a piece of mycelium into a substrate jar using my glove box??

I tried searching and cant really find anything off the bat, any help would be appreciated. :mushroom2: :mushroom2:


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Offlineazurescens
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Re: inoculation using Agar [Re: infamous]
    #3633679 - 01/16/05 01:41 AM (12 years, 8 months ago)

1st off, make sure you select the purest strain you can find. By this I refer to one that has good rhizomorphic growth. Then just cut a wedge or two out of it and put it into your substrate jar and shake well.


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Offlineinfamous
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Re: inoculation using Agar [Re: azurescens]
    #3633713 - 01/16/05 01:52 AM (12 years, 8 months ago)

yes iv already done that I just wasnt sure what the best method for inoculation would be.


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InvisiblePrisoner#1
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Re: inoculation using Agar [Re: infamous]
    #3634431 - 01/16/05 10:02 AM (12 years, 8 months ago)

a couple of methods work well... scoop the agar into a sterilzed blender with
a bit of distilled water, puree and make syringes.... slice it like a pizza and
use the wedges to inoculate grain jars


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Invisibleohmatic
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Re: inoculation using Agar [Re: Prisoner#1]
    #3634457 - 01/16/05 10:43 AM (12 years, 8 months ago)

jeppa you have a couple of options:
cut wedge / scoop surface to make syringe / blend wedge ...

if you decide to cut a wedge and kick it to a sterile jar,
you can expect it to 'fuzz up' within 3 days and start colonising
the grains on 4th onwards.

usually within a week or 10 days enuf kernels are colonised to
shake them proper and get a nice all over kolonisation.

if you throw in 3 or more wedges, youll drastically speed that process up :smile:
peace ohm :mushroom2:


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Amazon Shop: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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