Cloning Question (PIC) - Mushroom Cultivation

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Holydiver
Stranger

Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea

Cloning Question (PIC)

#3630321 - 01/15/05 07:21 AM (19 years, 3 months ago)

I plan on doing a clone from this cluster of GT on WBS, as per the 9er Tek Blender Clone and I have a question: These are fairly small and to make matters harder, the stems are hollow. It will be hard to get a good amount of flesh from the inner stem. Should I split the cap and get my culture from there, and proceed to drop that in the blender? Or is there more flesh available then I am aware of even with hollow stems? Here is a pic of the cluster I'm shooting for:

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tripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL

Re: Cloning Question (PIC)

[Re: Holydiver]
#3630554 - 01/15/05 10:11 AM (19 years, 3 months ago)

Are you "cloning" on agar , or planning to make a liquid culture from the flesh you harvest ?

If cloning on agar , YES even the smallest piece of flesh taken from inside the mush stem will work . And if doing this way , there is no need to blend it at all , just harvest small piece , lay it on the surface of the agar , and watch it grow .

GL
PS sorry to lazy to read the link you posted :rolleyes:

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LaughingJim
Anti-Toxin

Registered: 09/30/04
Posts: 465
Loc: USA, ( CT )
Last seen: 18 years, 7 months

Re: Cloning Question (PIC)

[Re: tripndicular]
#3630566 - 01/15/05 10:15 AM (19 years, 3 months ago)

All the TEKs that I read said to peel teh cap-skin back and get a sample of flesh from there. (Might be better, or safer.)

Do both!

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Holydiver
Stranger

Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea

Re: Cloning Question (PIC)

[Re: tripndicular]
#3630572 - 01/15/05 10:17 AM (19 years, 3 months ago)

Quote:

tripndicular said:
PS sorry to lazy to read the link you posted

Thanks anyways, but if you read the link you'd know that I'm planning on making a mycelium slur out of the flesh in the blender, not agar work. :crazy:

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MushroomFriend
I smell a conspiracy!

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days

Re: Cloning Question (PIC)

[Re: Holydiver]
#3630705 - 01/15/05 11:06 AM (19 years, 3 months ago)

One reason they take the inside is cause it is sterile and the outside isnt.
IMHO you dont need to blend, you can also drop a piece (or slice that in 4 parts or sumtin) in the liquid substrate. You might dip it in dilluted h202 before that, about 0,3% solution. Which is 1 part 3% h2o2 on 10 parts water.

But I think for development agar is better then LQ. To make inoculant I think LQ could be great. I am having trouble getting the thick mycelium in the syringe though.......

Good luck.

MF

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Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379

Re: Cloning Question (PIC)

[Re: MushroomFriend]
#3630788 - 01/15/05 11:30 AM (19 years, 3 months ago)

I cloned an Enokie once from inside the stem and the stem was about as thick as a pencil lead!
a bit as big as this ( = )
turned into this...

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NOS4A2
This is the way

Registered: 07/22/04
Posts: 572
Loc: -tite

Re: Cloning Question (PIC)

[Re: MushroomFriend]
#3630792 - 01/15/05 11:31 AM (19 years, 3 months ago)

MF did you end up getting those syringes at rymed? Just wondering if they seemed to small a guage for the Lq cult

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MushroomFriend
I smell a conspiracy!

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 month, 8 days

Re: Cloning Question (PIC)

[Re: Holydiver]
#3631266 - 01/15/05 01:36 PM (19 years, 3 months ago)

Yow NOS!

No, untill now I use the syringes from the several sporesyringes I have bought!

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