Home | Community | Message Board


Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: Agar, Wild Bird Seed

Jump to first unread post. Pages: 1
InvisibleHolydiver
Stranger
 User Gallery

Folding@home Statistics
Registered: 03/20/01
Posts: 5,156
Loc: The midnight sea
Trusted Cultivator
Cloning Question (PIC)
    #3630321 - 01/15/05 09:21 AM (12 years, 5 months ago)

I plan on doing a clone from this cluster of GT on WBS, as per the 9er Tek Blender Clone and I have a question: These are fairly small and to make matters harder, the stems are hollow. It will be hard to get a good amount of flesh from the inner stem. Should I split the cap and get my culture from there, and proceed to drop that in the blender? Or is there more flesh available then I am aware of even with hollow stems? Here is a pic of the cluster I'm shooting for:


--------------------
To find a place to live between the negatives and positives.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Cloning Question (PIC) [Re: Holydiver]
    #3630554 - 01/15/05 12:11 PM (12 years, 5 months ago)

Are you "cloning" on agar , or planning to make a liquid culture from the flesh you harvest ?

If cloning on agar , YES even the smallest piece of flesh taken from inside the mush stem will work . And if doing this way , there is no need to blend it at all , just harvest small piece , lay it on the surface of the agar , and watch it grow .

GL
PS sorry to lazy to read the link you posted  :rolleyes: :wink:


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineLaughingJim
Anti-Toxin
 User Gallery

Registered: 09/30/04
Posts: 465
Loc: USA, ( CT )
Last seen: 11 years, 9 months
Re: Cloning Question (PIC) [Re: tripndicular]
    #3630566 - 01/15/05 12:15 PM (12 years, 5 months ago)

All the TEKs that I read said to peel teh cap-skin back and get a sample of flesh from there. (Might be better, or safer.)

Do both!


--------------------
Don't forget to RATE us if you think we are offering GOOD or BAD advice, Thank-you!

I participate in the PROTEIN FOLDING PROGRAM helping the shroomery to help others,
FOLDING.STANFORD.EDU
I support www.shroomery.org (Team # 12679)


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleHolydiver
Stranger
 User Gallery

Folding@home Statistics
Registered: 03/20/01
Posts: 5,156
Loc: The midnight sea
Trusted Cultivator
Re: Cloning Question (PIC) [Re: tripndicular]
    #3630572 - 01/15/05 12:17 PM (12 years, 5 months ago)

Quote:

tripndicular said:
PS sorry to lazy to read the link you posted  :rolleyes: :wink:




Thanks anyways, but if you read the link you'd know that I'm planning on making a mycelium slur out of the flesh in the blender, not agar work.  :crazy:


--------------------
To find a place to live between the negatives and positives.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 6 months
Re: Cloning Question (PIC) [Re: Holydiver]
    #3630705 - 01/15/05 01:06 PM (12 years, 5 months ago)

One reason they take the inside is cause it is sterile and the outside isnt.
IMHO you dont need to blend, you can also drop a piece (or slice that in 4 parts or sumtin) in the liquid substrate. You might dip it in dilluted h202 before that, about 0,3% solution. Which is 1 part 3% h2o2 on 10 parts water.

But I think for development agar is better then LQ. To make inoculant I think LQ could be great. I am having trouble getting the thick mycelium in the syringe though.......

Good luck.

MF


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePeterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
Re: Cloning Question (PIC) [Re: MushroomFriend]
    #3630788 - 01/15/05 01:30 PM (12 years, 5 months ago)

I cloned an Enokie once from inside the stem and the stem was about as thick as a pencil lead!
a bit as big as this  (  =  )
turned into this...

:jester:


--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleNOS4A2
Sh!tApple

Registered: 07/22/04
Posts: 510
Loc: notfarfromtheshittree Flag
Re: Cloning Question (PIC) [Re: MushroomFriend]
    #3630792 - 01/15/05 01:31 PM (12 years, 5 months ago)

MF did you end up getting those syringes at rymed? Just wondering if they seemed to small a guage for the Lq cult


--------------------
Hail to the King, Baby
CulturingTech


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 6 months
Re: Cloning Question (PIC) [Re: Holydiver]
    #3631266 - 01/15/05 03:36 PM (12 years, 5 months ago)

Yow NOS!

No, untill now I use the syringes from the several sporesyringes I have bought!


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: Agar, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* cloning question...mycelium vs. single mushroom Mark_W 899 3 06/23/07 08:50 PM
by RogerRabbit
* Cloning question heed 447 1 09/20/06 09:00 PM
by RogerRabbit
* why do my clones grow in clusters? goldieman 843 1 11/13/07 07:09 PM
by bait_
* agar again and clone questions
anakin3197
581 8 03/13/09 02:21 PM
by anakin3197
* Cloning question - saving a clone subject? *DELETED* Ophanim 727 6 12/17/07 09:57 AM
by FooMan
* Noob cloning question Jon 671 5 12/30/05 10:07 PM
by Jon
* dumb liquid culture cloning question Anonymous 1,543 9 11/17/04 12:29 PM
by derx
* Really quick cloning question peruvian spark 565 4 03/03/04 09:24 PM
by RogerRabbit

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
921 topic views. 25 members, 108 guests and 39 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Zamnesia.com
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.038 seconds spending 0.007 seconds on 19 queries.