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Amazon Shop for: ½ Pint Jars, Rye Grain

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Invisiblemadsickpenguin
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Registered: 08/29/04
Posts: 234
G2G, need some advice
    #3629945 - 01/15/05 03:27 AM (11 years, 10 months ago)

First of all, AFOAF'S TIRED OF GETTING SCREWED BY A CERTAIN VENDOR, so he's not buying from him anymore.  The entire first batch of syringes he gave him were contammed.  Everything. 

He sent AFOAF saw no spore clumps in syringes at first but didn't think anything of it.  Oh well, f*ck him.

Anyways, AFOAF'S question is, these jars of rye that didn't have any spores germinate..can he use substrate from another jar for G2G?  It's been about 10 days and he was wondering if it was ok to still do it.  Thanks! :mushroom2: :mushroom2: :mushroom2: :mushroom2:


Edited by madsickpenguin (01/18/05 02:01 PM)


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Invisiblemuse_sick
ĤŌĿŶĞЋ0$Ŧ

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Re: G2G, need some advice [Re: madsickpenguin]
    #3629947 - 01/15/05 03:29 AM (11 years, 10 months ago)

no
i wouldn't try to do g2g 10 days after sterilizing
just make up fresh jars to transfer to


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Offlinefresh313
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Re: G2G, need some advice [Re: muse_sick]
    #3629955 - 01/15/05 03:35 AM (11 years, 10 months ago)

if there arent contams growing in the jars then sure, put some colonized grains in them. just be sterile during the transfer. wipe your tools down with alcohol and spray some lysol around.


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Invisiblemadsickpenguin
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Re: G2G, need some advice [Re: fresh313]
    #3629960 - 01/15/05 03:37 AM (11 years, 10 months ago)

AFOAF got a nice big glovebox he made, probably just use that. He thinks he's going to sterilize more grain, though. He's going G2G to the 10 day old jars and 4 more new jars, just to see if contam plays a greater factor in the old jars.


Edited by madsickpenguin (01/18/05 01:59 PM)


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Offlinefresh313
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Re: G2G, need some advice [Re: madsickpenguin]
    #3629964 - 01/15/05 03:40 AM (11 years, 10 months ago)

shouldnt make a difference unless the spores were contamed...
good luck


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Invisiblemuse_sick
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Re: G2G, need some advice [Re: madsickpenguin]
    #3629967 - 01/15/05 03:41 AM (11 years, 10 months ago)

my prediction: yes it does
experimenting is always fun though
just make sure to transfer to the new jars first to minimize possible contam exposure
btw: you are sure yr moisture content was right when you innoced the jars?
everytime i have had problems with grain not colonizing it has been as a result of moisture content being off
are you draining your grain for long enough before sterilization?


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Invisiblemadsickpenguin
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Re: G2G, need some advice [Re: muse_sick]
    #3629978 - 01/15/05 03:47 AM (11 years, 10 months ago)

Yes I'm sure the moisture content is quite right. Took AFOAF a few times to get it. At first he was not getting colonization over the whole jar due to too dry of grain. But he's pretty much got it down now.

The reason he believes it to be the syringes is because, he sterilzed 4 quarts of rye. Used up a B+ syringe on two jars (which appears to be going well right now, last batch of B+ ALL contammed) and then used a Koh Sumai on the last two jars.

Also, the next batch of 4 quart rye jars were knocked up usin' koh sumai and still nothing with them either. They just sit there lookin' like they want to germinate..but won't!!

Good idea, though, he will g2g the new jars first.


Edited by madsickpenguin (01/18/05 01:58 PM)


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Amazon Shop for: ½ Pint Jars, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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