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Cenebite
Closet CaseShroomer
Registered: 10/27/04
Posts: 348
Last seen: 7 years, 7 months
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WBS Knocked up 3.5 days ago, no signs of growth?
#3619876 - 01/12/05 06:26 PM (19 years, 2 months ago) |
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I PC'ed and innoced 7 pint jars of WBS with about 2.5-3cc's of spore solution in each, I then shook the jars vigorulsly and now there being incubated at 86 degrees. Generally how long should I wait to see growth? in a few jars I see the tiniest specks of mycellium.. I would think they'd take a bit longer then PF since they were shook up and theres no one major inocculation point, so the middle could be colonizing... with your expertise in WBS how long do you generally wait to notice visibile growth?
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Holydiver
Stranger
Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3619922 - 01/12/05 06:38 PM (19 years, 2 months ago) |
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Germination time is the same as any other substrate. You're seeing tiny specks of mycelium at 3.5 days? Sounds like you're on track then. It can take 5 days or longer. Patience
-------------------- To find a place to live between the negatives and positives.
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JaRRn
Lost in Space
Registered: 05/20/04
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Loc: Standing on the Cosmic Sh...
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3619946 - 01/12/05 06:42 PM (19 years, 2 months ago) |
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onetime
onetime
Registered: 11/13/03
Posts: 3,609
Last seen: 13 years, 3 months
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: JaRRn]
#3619968 - 01/12/05 06:45 PM (19 years, 2 months ago) |
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wbs takes 7-11 days
-------------------- See? Yes, with my own three eyes. Depression, Misspells , wanting everying thing i cant have haveing nothing i want
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recalcitrant
My Own God
Registered: 04/20/02
Posts: 2,927
Loc: Canada West
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3620004 - 01/12/05 06:52 PM (19 years, 2 months ago) |
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You're bloody lucky to see anything inside a week.
-------------------- We have to answer our own prayers
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Cenebite
Closet CaseShroomer
Registered: 10/27/04
Posts: 348
Last seen: 7 years, 7 months
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: recalcitrant]
#3620118 - 01/12/05 07:06 PM (19 years, 2 months ago) |
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Awsome guys thanks so much, i've never got a contam or a non colonizing jar so im worried my streak might of been over haha but knock on wood anyways! Thanks...
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3620174 - 01/12/05 07:13 PM (19 years, 2 months ago) |
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You will see colonization quicker, if you innoc a squirt in each corner of a jar (against the glass) & 1 down the center. Rather than innoc & shake. EXAMPLE:
Shake when about 15% colonized.
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IGnosticAbhorI
Stranger-er
Registered: 11/06/04
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: agar]
#3620503 - 01/12/05 08:21 PM (19 years, 2 months ago) |
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Or you could just do a g2g and not even have to wait for germination =)
atleast that's my .02 anyways....
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kiss
numbnuts
Registered: 12/14/04
Posts: 486
Loc: under your bed
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: recalcitrant]
#3620805 - 01/12/05 09:23 PM (19 years, 2 months ago) |
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This jar was noc'ed up 7 days ago with 2cc's... And this one 6 days ago...
-------------------- see dick drink see dick drive see dick die don't be a dick!
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Cenebite
Closet CaseShroomer
Registered: 10/27/04
Posts: 348
Last seen: 7 years, 7 months
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: kiss]
#3620837 - 01/12/05 09:31 PM (19 years, 2 months ago) |
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were those both WBS? The reason I cant do G2G is because I have no grain to transfer haha these are my first grain jars..
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Cenebite
Closet CaseShroomer
Registered: 10/27/04
Posts: 348
Last seen: 7 years, 7 months
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3620841 - 01/12/05 09:32 PM (19 years, 2 months ago) |
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How often do you shake up WBS jars? if at all that is?
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IGnosticAbhorI
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3620850 - 01/12/05 09:34 PM (19 years, 2 months ago) |
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Wow, 2cc's for a pint?
I typically use just 1.5CC's...lol, .5 less, but if you knock up 10 jars...then you saved like what, 5CCs?
Well, better safe than sorry i guess, nice jars btw
Def. looks like wbs to me
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3620860 - 01/12/05 09:35 PM (19 years, 2 months ago) |
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shake any jar after you see that the mycel has recovered from the previous shake and it is growing on the surrounding kernels. As for time. I just saw growth on a jar that i shot up on christmas
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you. My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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kiss
numbnuts
Registered: 12/14/04
Posts: 486
Loc: under your bed
Last seen: 9 years, 3 months
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: tahoe]
#3620874 - 01/12/05 09:38 PM (19 years, 2 months ago) |
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Actually the pic. of the jar at 6 days is my own slight var. of PF tek. the millet based seed is the jar at 7 days today. I don't mind 2cc a jar i'm gonna get it back anyway 10 fold
-------------------- see dick drink see dick drive see dick die don't be a dick!
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IGnosticAbhorI
Stranger-er
Registered: 11/06/04
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: kiss]
#3620915 - 01/12/05 09:45 PM (19 years, 2 months ago) |
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Millet, wbs, same dif..lol..kinda
And yea, that's true
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