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Cenebite
Closet CaseShroomer


Registered: 10/27/04
Posts: 348
Last seen: 8 years, 5 months
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WBS Knocked up 3.5 days ago, no signs of growth?
#3619876 - 01/12/05 06:26 PM (20 years, 8 days ago) |
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I PC'ed and innoced 7 pint jars of WBS with about 2.5-3cc's of spore solution in each, I then shook the jars vigorulsly and now there being incubated at 86 degrees. Generally how long should I wait to see growth? in a few jars I see the tiniest specks of mycellium.. I would think they'd take a bit longer then PF since they were shook up and theres no one major inocculation point, so the middle could be colonizing... with your expertise in WBS how long do you generally wait to notice visibile growth?
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Holydiver
Stranger



Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3619922 - 01/12/05 06:38 PM (20 years, 8 days ago) |
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Germination time is the same as any other substrate. You're seeing tiny specks of mycelium at 3.5 days? Sounds like you're on track then. It can take 5 days or longer. Patience
-------------------- To find a place to live between the negatives and positives.
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JaRRn
Lost in Space


Registered: 05/20/04
Posts: 1,155
Loc: Standing on the Cosmic Sh...
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3619946 - 01/12/05 06:42 PM (20 years, 8 days ago) |
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onetime
onetime


Registered: 11/13/03
Posts: 3,609
Last seen: 14 years, 1 month
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: JaRRn]
#3619968 - 01/12/05 06:45 PM (20 years, 8 days ago) |
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wbs takes 7-11 days
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See?
Yes, with my own three eyes.
Depression, Misspells , wanting everying thing i cant have haveing nothing i want
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recalcitrant
My Own God

Registered: 04/20/02
Posts: 2,927
Loc: Canada West
Last seen: 8 years, 8 months
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3620004 - 01/12/05 06:52 PM (20 years, 8 days ago) |
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You're bloody lucky to see anything inside a week.
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We have to answer our own prayers
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Cenebite
Closet CaseShroomer


Registered: 10/27/04
Posts: 348
Last seen: 8 years, 5 months
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: recalcitrant]
#3620118 - 01/12/05 07:06 PM (20 years, 8 days ago) |
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Awsome guys thanks so much, i've never got a contam or a non colonizing jar so im worried my streak might of been over haha but knock on wood anyways! Thanks...
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3620174 - 01/12/05 07:13 PM (20 years, 8 days ago) |
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You will see colonization quicker, if you innoc a squirt in each corner of a jar (against the glass) & 1 down the center. Rather than innoc & shake. EXAMPLE:
 Shake when about 15% colonized.
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IGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: agar]
#3620503 - 01/12/05 08:21 PM (20 years, 8 days ago) |
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Or you could just do a g2g and not even have to wait for germination =)
atleast that's my .02 anyways....
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kiss
numbnuts


Registered: 12/14/04
Posts: 486
Loc: under your bed
Last seen: 10 years, 1 month
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: recalcitrant]
#3620805 - 01/12/05 09:23 PM (20 years, 8 days ago) |
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This jar was noc'ed up 7 days ago with 2cc's...  And this one 6 days ago...
-------------------- see dick drink
see dick drive
see dick die
don't be a dick!
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Cenebite
Closet CaseShroomer


Registered: 10/27/04
Posts: 348
Last seen: 8 years, 5 months
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: kiss]
#3620837 - 01/12/05 09:31 PM (20 years, 8 days ago) |
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were those both WBS? The reason I cant do G2G is because I have no grain to transfer haha these are my first grain jars..
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Cenebite
Closet CaseShroomer


Registered: 10/27/04
Posts: 348
Last seen: 8 years, 5 months
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3620841 - 01/12/05 09:32 PM (20 years, 8 days ago) |
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How often do you shake up WBS jars? if at all that is?
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IGnosticAbhorI
Stranger-er

Registered: 11/06/04
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3620850 - 01/12/05 09:34 PM (20 years, 8 days ago) |
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Wow, 2cc's for a pint?
I typically use just 1.5CC's...lol, .5 less, but if you knock up 10 jars...then you saved like what, 5CCs?
Well, better safe than sorry i guess, nice jars btw 
Def. looks like wbs to me
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tahoe
Noob Slayer


Registered: 11/26/03
Posts: 6,285
Loc: N38.93829W119.98108
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: Cenebite]
#3620860 - 01/12/05 09:35 PM (20 years, 8 days ago) |
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shake any jar after you see that the mycel has recovered from the previous shake and it is growing on the surrounding kernels. As for time. I just saw growth on a jar that i shot up on christmas
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987
Teh=The
I need to proofread
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kiss
numbnuts


Registered: 12/14/04
Posts: 486
Loc: under your bed
Last seen: 10 years, 1 month
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: tahoe]
#3620874 - 01/12/05 09:38 PM (20 years, 8 days ago) |
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Actually the pic. of the jar at 6 days is my own slight var. of PF tek. the millet based seed is the jar at 7 days today. I don't mind 2cc a jar i'm gonna get it back anyway 10 fold
-------------------- see dick drink
see dick drive
see dick die
don't be a dick!
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IGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 1 year, 7 months
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Re: WBS Knocked up 3.5 days ago, no signs of growth? [Re: kiss]
#3620915 - 01/12/05 09:45 PM (20 years, 8 days ago) |
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Millet, wbs, same dif..lol..kinda
And yea, that's true
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