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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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OfflineDatrutankdogg69
Cyan Man
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Registered: 01/11/01
Posts: 202
Loc: Pacific NW, Or.
Last seen: 5 years, 3 months
New Tek!!??
    #360112 - 07/24/01 03:18 PM (22 years, 2 months ago)

If you havesome myc. in a jar on agar and wanted 2 make a syinge could you:

Mixup a water/peroxide solution?(What ratio?),
Pour into jar of agar/Myc.,
Shake well,
Use half full honey syringe(Pre-sterilized in microwave) 2 suck up myc./H2O2 mixture
and come out with a completely sterile syinge???

Curious cause I just tried this one today was wondering if any1 else has.
Thanx ppl!

'Clown Wit It!!'~~ MYSTIKAL


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InvisibleBrownPastures
old hand

Registered: 03/15/01
Posts: 968
Loc: here
Re: New Tek!!?? [Re: Datrutankdogg69]
    #360120 - 07/24/01 03:36 PM (22 years, 2 months ago)

No not a new tek. Seems like an OLD tek with a whole lotta unnecessary steps. If you have some mycelium in a jar you make honey water or something like that. Transfer your mycelium to that jar. Then use it as an innoculant. Other than that this, post(?), is halfway from nowhere to nowhere else. Peace

"A Wise One like me should not charge for her services....The one who charges is a liar. The wise one is born to cure, not to do business with her knowledge."-Maria Sabina


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OfflineDatrutankdogg69
Cyan Man
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Registered: 01/11/01
Posts: 202
Loc: Pacific NW, Or.
Last seen: 5 years, 3 months
Re: New Tek!!?? [Re: Datrutankdogg69]
    #360125 - 07/24/01 03:52 PM (22 years, 2 months ago)

Finally a fukkin' answer.

What I have is an old jar of myc. on agar that may have become contam but don't know because it's completely colonised. So I figuered if I used A water/h2o2 solution to sterilize myc. I could then suck myc./h2. into presterilized malt syinge with no chance of contam. Oh yea, did I forget to mention that I'm doin' all this without a glovebox.

I would've mentioned all this in the first place but I noticed alot of ppl on here get scared when they see a paragraph longer than 5 or 6 lines long!!! ha ha

Later bp

'Clown Wit It!!'~~ MYSTIKAL


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