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Amazon Shop for: ½ Pint Jars, Agar, Pressure Cooker

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Offlineboise
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Registered: 12/14/04
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Green Mold?
    #3588112 - 01/06/05 10:31 AM (11 years, 10 months ago)

Hi. I'm sure this question's been asked a thousand times, but here it is again.

I've got half-pint jars in incubation. One of them has a very obvious dime-sized patch of green mold. I've taken it out. 3 of the other jars, which are much more colonized than the infected jar (5 days, but much farther along than any of the other jars in the incubator) have the tiniest green flecks on the mycelium.

Can I be pretty sure this is mold and the jars need to be taken out before they infect everything else? Or do I wait a day and see if the mold takes over? I took a picture, but it's impossible to see it.

Thanks much.


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Offlinelemon_lw
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Re: Green Mold? [Re: boise]
    #3588128 - 01/06/05 10:35 AM (11 years, 10 months ago)

seperate them and wait a couple of days and you will find out. but if there is suspicion there is no reason to leave them in there cause it can spread.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlineboise
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Re: Green Mold? [Re: lemon_lw]
    #3588171 - 01/06/05 10:47 AM (11 years, 10 months ago)

Is it alright to take them out of incubation to wait and see? I'm going to have to put them in the back of the closet, cause I'm out of space. That'll just slow down the mycelium, but if there's mold in there, the mold will take over anyway?


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Offlinelemon_lw
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Re: Green Mold? [Re: boise]
    #3588194 - 01/06/05 10:51 AM (11 years, 10 months ago)

yeah if its mold it will take over. try to find something to put them in so they wont get more contams, maybe a ziploc bag. so take them out now, hurry, jk. but take them out asap.


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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InvisiblePeterthinks
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Re: Green Mold? [Re: lemon_lw]
    #3588217 - 01/06/05 10:58 AM (11 years, 10 months ago)

You could try PCing it again and re innoculating it.
I had a batch of agar I was keeping in a jar and I saw bacteria growing on top so I pressure cooked it (15psi for45),gave it a good shake and it's good to go.
Good thing too,it was like the last of my agar and about a liter of it!
Add some water(with syringe),PC the jar,incubate it for 4 days to make sure it's clean and try again.
really what have you got to lose except a CC of spores?
It may work...let us know if you try this untested method.
:jester:


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OfflineMcHurley
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Re: Green Mold? [Re: lemon_lw]
    #3588219 - 01/06/05 10:59 AM (11 years, 10 months ago)

I think you should incubate in total darkness. mycelium don't need light, mold does


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Offlineboise
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Re: Green Mold? [Re: lemon_lw]
    #3588223 - 01/06/05 11:00 AM (11 years, 10 months ago)

Ha, I'm running! Thanks for the bag idea. Don't know why that didn't occur to me. Would you ever recommend putting new jars in bags in the incubator as an added layer of protection? I've got a tiny rubbermaid tub in tub in a closet, big enough for 8 half-pints. Or does it seem likely that the mold was in there before going into the incubator?

That's my suspicion, at least. This is my first grow and there are a couple of points in the process where I feel like I may have made minor mistakes that could have lead to this happening.

Also, another question for you, now that I'm thinking back through the process: if the syringes are capped and in ziploc bags in the fridge, are they susceptible to contaminates as well? I realize sterilizing the needle before inoculation kills whatever's landed there when the cap came off, but can anything "crawl" up the needle and into the solution while it's sitting in the fridge?

Thanks again.


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Offlinelemon_lw
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Re: Green Mold? [Re: boise]
    #3588235 - 01/06/05 11:07 AM (11 years, 10 months ago)

dont use bags the whole time it will not allow good air exchange, well eventually no air exchange. and dont worry about the syringe, ive never heard of it happening and very highly doubt it could. next time remember sterilitty cause its so easy to contam if you dont pay close attention. flame the needle between every jar (the most common newb mistake).


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlineboise
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Re: Green Mold? [Re: lemon_lw]
    #3588258 - 01/06/05 11:18 AM (11 years, 10 months ago)

Thanks. I did flame between every jar, but now I'm thinking I may have mucked things a bit with the pressure cooker. Also, is there any truth to the mold needs light post above? I haven't seen that before. My incubator tubs are clear, but they're lined with towels and in a closed closet. I'm sure some tiny amount of light seeps in but is that nelgligible?


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Offlinelemon_lw
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Re: Green Mold? [Re: boise]
    #3588275 - 01/06/05 11:22 AM (11 years, 10 months ago)

ive never heard of the mold needs light. and while incubating in darkness a jar gets contamed so im guessing not but i could be wrong. i didnt understand where that came from but yeah when you isolate them make sure they are still dark cause you dont want them to start pinning on you.


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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OfflineZeroArmy27
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Re: Green Mold? [Re: lemon_lw]
    #3588475 - 01/06/05 12:28 PM (11 years, 10 months ago)

that guy's a one post dumbass. ignore his idiotic post.

it is possible your syringe was contamed. i got the meanie greenie in most of my jars one round and it ended up that the syringe was contamed... :frown:

isolate the suspected jars. see if it is green mold or not.


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Offlinepseudorhiza
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Re: Green Mold? [Re: boise]
    #3588511 - 01/06/05 12:43 PM (11 years, 10 months ago)

Quote:

boise said:
Hi. I'm sure this question's been asked a thousand times, but here it is again.

I've got half-pint jars in incubation. One of them has a very obvious dime-sized patch of green mold. I've taken it out. 3 of the other jars, which are much more colonized than the infected jar (5 days, but much farther along than any of the other jars in the incubator) have the tiniest green flecks on the mycelium.

Can I be pretty sure this is mold and the jars need to be taken out before they infect everything else? Or do I wait a day and see if the mold takes over? I took a picture, but it's impossible to see it.

Thanks much.





If you got green you can see , count this one as a failure . :eek:
You may need to work on sterilization practices more before moving on again , that or your spore source was contaminated from the get go .
Make up a batch of sub , let it sit for a few days , does this new batch have nasties showing up ? If so you did not sterilize properly .
If you get to the inoc point and this is when contams start showing up , then it is your inoc procedure and cleanliness , or bad syringe .
Lots of angles to determine "exactally" how and where nasties came in , but in time you will find the source of your troubles , in most cases it is the preparer  :wink:
Good Luck
Again , I say start over , issolate those suckers or kill them ASAP !


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InvisiblePrisoner#1M
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Re: Green Mold? [Re: McHurley]
    #3588526 - 01/06/05 12:49 PM (11 years, 10 months ago)

Quote:

McHurley said:
I think you should incubate in total darkness. mycelium don't need light, mold does




mold certainly does not need light, explain the black mold inside the walls and
under the flooring of houses... I've yet to find a mold that requires light


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Offlinepseudorhiza
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Re: Green Mold? [Re: McHurley]
    #3588593 - 01/06/05 01:11 PM (11 years, 10 months ago)

Quote:

McHurley said:
I think you should incubate in total darkness. mycelium don't need light, mold does




This post does not ask about this but if myc does not need light ....
Explain the pin initiation process  :confused:

Molds can and will grow in any enviro , lighted or not , with air or no air , moisture is the only prerequisite I am aware of .
You need to get some basics down before you try to give anyone answers , even half assed ones  :rolleyes:


--------------------
"Toxic Shock Syndrome is God's way of punishing unsaved harlots who choose Satan?s cotton fingers over a Godly pad," Pastor Deacon Fred stated upon hearing of the event. "These playthings of Satan are created under the guise of a ladies hygiene product to bring unsuspecting women and young girls to the fold of the Devil."


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OfflinePorchMonkey321
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Re: Green Mold? [Re: McHurley]
    #3588759 - 01/06/05 01:55 PM (11 years, 10 months ago)

Quote:

McHurley said:
I think you should incubate in total darkness. mycelium don't need light, mold does




Wtf? Last time I checked, unless you have high-dollar low-light optical equipment, you need light to see what the hell you're doing... What a way to start posting at the Shroomery, with a dumbass answer such as that. Think about what you say bub, maybe you wont look like a total idiot next time you post.


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Offlineboise
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Re: Green Mold? [Re: PorchMonkey321]
    #3588786 - 01/06/05 02:00 PM (11 years, 10 months ago)

Thanks for all the replies. I'll be sure to cook the jars and then watch them before innoculating in the future. Would definitely like to find the source...


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OfflineLaughingJim
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Re: Green Mold? [Re: boise]
    #3589381 - 01/06/05 05:14 PM (11 years, 10 months ago)

If you see a dime size outside, there is a golf-ball clump inside the cake.


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OfflineLaughingJim
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Re: Green Mold? [Re: LaughingJim]
    #3589401 - 01/06/05 05:21 PM (11 years, 10 months ago)

Mold likes dark and humid, hates light and arid. (However, it can withstand light and arid.)

The only natural way to kill mold spores without chemicals is to make sure they have absorbed all the moisture they can, then blow them up with extreme temps. (Soak for 24 hours, under pressure if possible. Then PC at 220F+ for 45 minutes at 10 PSI minimum.)

Totally dry spores can withstand temps up to 800F+, for a certain time. Pressure treated, kiln-dried wood can still rot from the inside-out. Once moisture penetrates, it activates the dried spores.

EXAMPLE, dry your hand and wave it through the flame of a torch real fast. (You will feel a little heat.) Now, soak your hand in warm water for an hour, until it wrinkles. Then pass your hand through the torch with the same speed and at the same distance. (Your hand will instantly blister, and your skin will peel off for weeks.)

You have instantly boiled the water inside your skin, that your hand absorbed from soaking. Where as before, you only boiled the water on the surface of your skin, the oil inside your skin didn't absorb the heat as fast, so you felt warmth, and not searing.


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Edited by LaughingJim (01/06/05 05:28 PM)


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