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 agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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 (TLG) UTRASONICLLY TREATED SPORES 
#3579789 - 01/04/05 02:29 PM (19 years, 3 months ago) |
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UTRASONICLLY TREATED SPORES & SPOREBAGS:
Agar long ago received his first successful spore batch from TLG. Over the years, he received many differing strains from TLG, without ever a problem in orders.
TLG gets fecal matter thrown at him from another vendor - because TLG is introducing into his inventory NEXT GENERATION, I., E., Ultrasonically treated spore syringes. Which is said to disperse spores into a solution incredibly well.
Well, agar saw so much fecal matter thrown at TLG, he decided to have his foaf give it a test. Just to see for himself. Agar?s foaf makes PRINTS on autoclave bag material (SporeBags). He does so for several reasons.
1. It is easy. 2. Autoclave bag material is inexpensive. 2. Autoclave bag material can be SEALED with an impulse sealer. Net result is a print, sealed in a sterile waterproof material, that is impervious to contaminates.
To test TLG?s UTRASONIC DISPERSAL method. Agars foaf used a piece of autoclave bag material measuring 6 inches wide & a 12 inches long, then set 2 big fat healthy EQ caps on it & made a double print - which he allowed to dry 3 days.
He then folded the bag in half (prints facing each other) & impulse sealed the bag. He then injected 40ml of sterile water into the bag (on a corner), then resealed the bag (impulse sealer). He then dropped the bag into an a water filled ultrasonic cleaner, turned it on & let it bath overnight. Next morning - he found THIS:
It works  , NUFF SAID.
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 13eetleJuice
the ghost with the most
Registered: 10/28/04
Posts: 2,253
Loc: 6' under pushin up shroom...
Last seen: 5 years, 4 months
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 Re: (TLG) UTRASONICLLY TREATED SPORES [Re: agar]
#3579865 - 01/04/05 02:44 PM (19 years, 3 months ago) |
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bumpidy
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KaptKid
Spaced Pirate
Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 4 years, 1 month
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: 13eetleJuice]
#3579894 - 01/04/05 02:51 PM (19 years, 3 months ago) |
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Now even more goodies to get.   
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Child of the 60's, Tripping ever since.
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SunshinePounder
The Girl withthe Sun in HerEyes ☺
Registered: 11/19/04
Posts: 288
Loc: Peace Man!
Last seen: 14 years, 7 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: 13eetleJuice]
#3579897 - 01/04/05 02:52 PM (19 years, 3 months ago) |
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Well said Agar
tlg's the man in my book
peace
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My [url=www.http://tinyurl.com/6valr target=_blank]Unitarian Jihad Name[/url] is: Sister Claymore of The Short Path
Get yours
If I was the sun, I'd look for the shade~Liberty
Baby I love you, and you love me. Let's love each other to eternity~
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NOS4A2
This is the way
Registered: 07/22/04
Posts: 572
Loc: -tite 
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OK where is this crap(equip) to be found?
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KaptKid
Spaced Pirate
Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 4 years, 1 month
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: NOS4A2]
#3580335 - 01/04/05 04:14 PM (19 years, 3 months ago) |
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I know they got something like that for dentures.agar were did you your's and what type???
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Child of the 60's, Tripping ever since.
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PawPaw
Picktish TexasStyle....
Registered: 12/20/04
Posts: 8,243
Loc: was born down in the sout...
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 Re: (TLG) UTRASONICLLY TREATED SPORES [Re: agar]
#3580353 - 01/04/05 04:18 PM (19 years, 3 months ago) |
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TLG makes me feel like a big girl ...
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Come to Paw Paws place there will be no sleeping tonight
Eric just wants to catch reds ..
Don?t look back, don?t look back
He?s right on your trail
Don?t look back, don?t look back
He?s just a step away from hell
WDYWFM?
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 3 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: NOS4A2]
#3580359 - 01/04/05 04:21 PM (19 years, 3 months ago) |
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ebay...ultrasonic jewlery cleaners. I'm working on winning a hot plate right now...then I'll be going for one of these. Liquid cultures I think will benifit even more from this...Agar..you should do up a batch of jars...some spores your regular way..some spores ultra soniced..some liq myc your regular way...some liq myc ultrasoniced..you get incredible results doing this as is so if there really is a difference we should see it there. Those 4 groups would make excellent pictures for TLG I'm sure too to show people with controls how it helps.
I'm sure I'll test it myself but I'm more of a liq culture person then spores..still havnt made a syringe yet..and I dont have the ultrasonic yet..I'll be trying it with and without with liquid myc once I do get one but hey I figured if you've got the time and the urge 
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NOS4A2
This is the way
Registered: 07/22/04
Posts: 572
Loc: -tite
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: PawPaw]
#3580363 - 01/04/05 04:23 PM (19 years, 3 months ago) |
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Quote:
PawPaw said:
TLG makes me feel like a big girl ...
OK am I the only one a little freaked out right now??(what is that? a breadstick?)
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: KaptKid]
#3580387 - 01/04/05 04:30 PM (19 years, 3 months ago) |
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Ebay. If you are patient? You can get a GOOD one with fairly large stainless steel tank, for cheap. Best get one that has a 2 quart or bigger container & stainless steel. Name brand on mine is BRANSON
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 3 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: agar]
#3580515 - 01/04/05 05:06 PM (19 years, 3 months ago) |
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Time to hunt down an ultrasonic
Edited by scatmanrav (01/04/05 06:39 PM)
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: scatmanrav]
#3580564 - 01/04/05 05:17 PM (19 years, 3 months ago) |
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I hear ya....BUT
My foaf does liquid culture with one of these.
Once so thick it will not stir any more. He pours it into one of these (sterilized).
Hit the blend button on pulse a few times & you have the HOTTEST - FASTEST INNOC in the world.
If you want even faster & less work. Just snip a whole stem off a big shroom grown invitro(bag or jar)so it is as CLEAN as possible, sink it into a peroxide bath for 15 / 20 minutes, remove & blend in sterile water.
Store for a week in jar.. to see if any contams show up & if not.... go for it.
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eric_the_red
Registered: 02/28/03
Posts: 14,560
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: agar]
#3580569 - 01/04/05 05:18 PM (19 years, 3 months ago) |
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i wish eberbachs weren't so damn expensive.
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Anno cock? is that some kind of Greek liqueur? -Geo's All Knowing Sex Slave
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: eric_the_red]
#3580628 - 01/04/05 05:30 PM (19 years, 3 months ago) |
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That stainless steel container happens to be a WARING (Pro bartender). I got it at a 2nd hand resturant eqt & supply place for $20 (grin). Agar the frugal
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 3 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: agar]
#3580662 - 01/04/05 05:37 PM (19 years, 3 months ago) |
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Oh yeah I forgot about that..
Its hard to find that sort of thing though..ultrasonics can be had for a cheap price easier...well you should still knock up some jars with those spores at the same time as some prepared the same way but without being ultrasoniced. Something to compare to is what alot of the naysayers want to see.
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 3 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: scatmanrav]
#3581053 - 01/04/05 06:48 PM (19 years, 3 months ago) |
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NOS4A2
This is the way
Registered: 07/22/04
Posts: 572
Loc: -tite
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: scatmanrav]
#3581144 - 01/04/05 07:08 PM (19 years, 3 months ago) |
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$15 for shipping?
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 3 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: NOS4A2]
#3581180 - 01/04/05 07:14 PM (19 years, 3 months ago) |
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Didnt notice that but thats still the cheapest I've seen yet...I'm open to links though
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ticktock
Seeker
Registered: 10/02/04
Posts: 389
Loc: Just ask the rabbit.
Last seen: 14 years, 1 month
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: scatmanrav]
#3581636 - 01/04/05 08:49 PM (19 years, 3 months ago) |
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Ultrasonic treated liquid culture.
You have my attention. I am doing liquid culture, (karo), from spores, and own a huge ultrasonic cleaner.
What, exactly, should I be doing?
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 Don't panic!
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 3 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: ticktock]
#3581845 - 01/04/05 09:45 PM (19 years, 3 months ago) |
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All you do is run it through the ultrasonic which vibrates it apart. Not sure how long it will take...anywhere from 30 seconds to 24 hours I'd imagine. If it works like spore dispursal then you should have an extremly broken up liquid culture.
This could also benifit growth somehow...either running it 5 minutes a day to agitate or perhaps even constantly for constant agitation. I'm not sure of what if any this would do but it could do good and IMO is worth experimenting with.
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