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NOS4A2
This is the way

Registered: 07/22/04
Posts: 572
Loc: -tite
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: scatmanrav]
#3581912 - 01/04/05 10:08 PM (20 years, 16 days ago) |
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Not to be a downer, JMO, I expect with liquid culture you will not see a noticable difference since unlike the spore culture it is not made up of many small divisible (seperate) units but actually a network of inter twined "ropes" that may prove to be a challenge for the sonic cleaner. Not to metion a foodsaver bag is quite thick and stiff when sealed. Like I said just my opinion so please prove me wrong.
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Last seen: 12 years, 14 days
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: NOS4A2]
#3581946 - 01/04/05 10:19 PM (20 years, 16 days ago) |
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The bag would not be an issue..you can put it in a jar, whatever, the ultrasonic cleaner will work just fine and the water within whatever you put it in. The ropiness of the mycelium may be an issue like you said..I've thought that..but I think that it may still be enough..it just may take longer. But besides that what if from inoculation it was left in the cleaner? Never forming one huge cloud but many many little ones..this may still be just as effective..or moreso. Or what if you manually shake it then throw it in the ultrasonic to break it up even further?
I see where your thoughts came from..I thought the same but this is what I reasoned after thinking about it. I certainly hope to prove you wrong at some point..that would be cool...
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: NOS4A2]
#3581962 - 01/04/05 10:24 PM (20 years, 16 days ago) |
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A thick liquid culture is a much stronger mass than you think. That is why I went to the expense of buying a stainless steel blender. 10 seconds of pulsing divides a large glob, into multi-millions of particles. Any one of which can get the job done. Millions get it done so fast, there is nothing to even compete with it.
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NOS4A2
This is the way

Registered: 07/22/04
Posts: 572
Loc: -tite
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: agar]
#3581999 - 01/04/05 10:34 PM (20 years, 16 days ago) |
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Hey, please try, man. I dare ya. But mycelium is living tissue and if the sonic had any effect and you left it in after innoc untill you needed it you'd be damaging it over and over with out a break for it to catch up and possibly creating mutations(genetic damage) which maybe a good thing to some people. And you should give it a try even if I make sense. Which might not happen often.
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13eetleJuice
the ghost with the most



Registered: 10/28/04
Posts: 2,253
Loc: 6' under pushin up shroom...
Last seen: 6 years, 1 month
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: agar]
#3582013 - 01/04/05 10:38 PM (20 years, 16 days ago) |
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These devices are used to clean decades of grit and grime off of jewelry in mere minutes. It's a very powerful instrument. Granted, it doesn't hold a candle to a blender set to puree for dividing liquid culture. However, this seems to be a fairly new technology in terms of it's use by mycological hobbyists and my main concern is with it's applications in spore dispersal.
I wonder if left on too long, does it damage any significant number of the spores by being bombarded by ultrasonics at a rate of upwards of 40,000 waves/second? Anyone have any thoughts on this? I don't know how strong the cell walls of any given spore are but it seems logical to me that it could damage a number of them over a period of time. The point I'm getting at is that there might be an optimal duration whereby once you pass this point you are doing more harm than good. Comments?
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NOS4A2
This is the way

Registered: 07/22/04
Posts: 572
Loc: -tite
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: 13eetleJuice]
#3582157 - 01/04/05 11:17 PM (20 years, 16 days ago) |
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DependI imagine wether you hydrate them first or sonic them right after putting them in water
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tripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: agar]
#3583426 - 01/05/05 09:30 AM (20 years, 15 days ago) |
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I have a question , hopefully a smart one that helps many with this idea . When buying Ultra sonic jewlery cleaner to use for this idea , do many of those use a heating element to help clean jewlery ? IF so how can one make sure they get one that does not overheat the spores ?
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .
Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: tripndicular]
#3583486 - 01/05/05 09:48 AM (20 years, 15 days ago) |
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The heat type (shaker) parts cleaner in a water bath, differs from a straight ultrasonic parts cleaner. Heat would not be a good thing for spores.
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boxtop703
ThesaurusLinguae Graecae


Registered: 06/26/01
Posts: 5,011
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: scatmanrav]
#3601510 - 01/09/05 12:40 AM (20 years, 11 days ago) |
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This is the model we'll be using for dispersal:
http://www.china-genesis.com/openwin.phtml?id=14&language=en
It's quite nice.
It holds 5 litres. I would suggest to everyone that if they're going to buy their own unit, make sure the inner tank is at least 9" long to fit the syringes.
If you presently have a smaller unit you can just put the spore fluid in a half pint jar and drop it in the tank that way.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: boxtop703]
#3601523 - 01/09/05 12:45 AM (20 years, 11 days ago) |
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Any idea how long those spore will stay in suspension using this new process? Just curious
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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ZeroArmy27
I didn't go to work for a month.

Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: hyphae]
#3601538 - 01/09/05 12:49 AM (20 years, 11 days ago) |
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that's what i was wondering. how is dispersing them going to help if they're going to settle during shipping?
-------------------- "a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut
"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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boxtop703
ThesaurusLinguae Graecae


Registered: 06/26/01
Posts: 5,011
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: hyphae]
#3601542 - 01/09/05 12:51 AM (20 years, 11 days ago) |
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Well I'm also adding an anti-clumping agent in the spore fluid before hitting them with the ultrasound.
The ultrasonic waves breaks the spore clumps down to a very fine powder and the anti-clumping agent keep them in an aqueous suspension seemingly indefinitely.
When looked at with the naked eye you will see the end product is very unique looking.
It's the two step process that I believe is the great breakthrough.
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ZeroArmy27
I didn't go to work for a month.

Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 10 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: boxtop703]
#3601594 - 01/09/05 01:04 AM (20 years, 11 days ago) |
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alright, that makes sense. kind of like using jet dry in your home made syringes... keeps them from getting super clumpy and sticking to the rubber stopper.
you've got me sold now.
-------------------- "a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut
"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: ZeroArmy27]
#3603898 - 01/09/05 02:36 PM (20 years, 11 days ago) |
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Quote:
ZeroArmy27 said: that's what i was wondering. how is dispersing them going to help if they're going to settle during shipping?
I'm quite sure they'll stay suspended far past shipping I'm wonder more on the lines of 30, 60, 90 days? The thing I've noticed when using JetDry it that they will still clump after time but heres the nice thing just a "tap" and they're completely dispersed again. I have to say that was kinda funny "settle during shippment"  BTW I'm laughing with you!
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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ricelicker
The Dude'sImaginary Friend

Registered: 03/31/00
Posts: 722
Loc: The Yard
Last seen: 3 years, 11 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: hyphae]
#3603952 - 01/09/05 02:48 PM (20 years, 11 days ago) |
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Well I know this improves colonization time but what, if any, are the other benefits?
-------------------- "my brain waves travel at half the speed afta we..." -del tha funkee
"Two men looked out through prison bars...one saw the mud, one saw the stars." -anonymous
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13eetleJuice
the ghost with the most



Registered: 10/28/04
Posts: 2,253
Loc: 6' under pushin up shroom...
Last seen: 6 years, 1 month
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: ricelicker]
#3603961 - 01/09/05 02:52 PM (20 years, 11 days ago) |
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Quote:
ricelicker said: Well I know this improves colonization time but what, if any, are the other benefits?
I for one hate trying to examine spores at high magnification when they're all clumped together like that. Much easier for viewing if they're dispersed well.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: boxtop703]
#3603962 - 01/09/05 02:52 PM (20 years, 11 days ago) |
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I put a large heavy complete print in a 60ml syringe, with a tiny drop of my own formula dispersal agent, just for fun. Then set it in the ultrasonic cleaner overnight. This is how it turned out.
 Even setting around for a few days, it stays that way. It settles a tiny bit, but one wrist snap shake & its back to BLACK.
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tripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: agar]
#3604003 - 01/09/05 03:00 PM (20 years, 11 days ago) |
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What dispersal agent are you using ?
Can I ask that ?
Oh shit this is a forbiden thread
I gotta go before...................I hear keys jingling do you , ah I must just be
Cool beans agar thanx for the time you took to show us all this concept !
-------------------- Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .
Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !
Edited by tripndicular (01/09/05 03:01 PM)
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 13 years, 8 months
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Re: (TLG) UTRASONICLLY TREATED SPORES [Re: ricelicker]
#3604044 - 01/09/05 03:06 PM (20 years, 11 days ago) |
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Quote:
ricelicker said: Well I know this improves colonization time but what, if any, are the other benefits?
Besides being easier to view under the scope it also helps prevent inconsistant inoculations often times resulting in some jars colonizing rapidly while others show little to no sign of growth. Of course we're talking when peeps forget to shake or shake insufficiently their syringes. Now everyone will have optimal spore dispersal whether they shake or not!!! This is a good day IMO!!! WOOT WOOT!!!!!!!
-------------------- Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE
"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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