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Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Original Sensible Seeds High THC Strains

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Offlinegray1
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Registered: 04/30/01
Posts: 430
Loc: brooklyn
Last seen: 21 years, 9 months
liquid culture failure * 1
    #357249 - 07/19/01 09:54 AM (22 years, 6 months ago)

afoaf innoculated 4 separate L bottles of media with 2 different strains (g. teacher, b+ sporeprints from sporeworks) (1/2 of each syringe into a bottle)
of the following media:
per L h20:
20g brf
20g oat flour
2.5g tryptone peptone
2.5g yeast extract

after several weeks, there are no signs of growth. afoaf is assuming that the problem was in the media, either the yeast extract or the tryptone.

any ideas as to what could have happened?

muchos gracias

gray1



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Offlinegray1
addict

Registered: 04/30/01
Posts: 430
Loc: brooklyn
Last seen: 21 years, 9 months
Re: liquid culture failure [Re: *HeGz*] * 1
    #357317 - 07/19/01 01:28 PM (22 years, 6 months ago)

there was no growth at all, be it fungal or bacterial or mamalian

to early or too late? as far as i know, microbiological media usually doesn't have short shelf life,
anyway, used it shortly after it was autoclaved, with more than sufficient cooling time.

does anyone know why yeast extract or tryptone peptone would inhibit fungal growth?





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Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Original Sensible Seeds High THC Strains


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