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Invisibleohmatic
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Re: Breaking up clumps of spores in syringe [Re: Prisoner#1]
    #3515284 - 12/17/04 09:43 PM (20 years, 1 month ago)

Quote:

Prisoner#1 said:
there are quite a few spores that can survive a 'killing' heat, some wont
germinate until that heat has been reached, there are also seeds
that will only germinate after they have been burned, these high temp
spores are something that many of us will never have to worry about
but they do exist




so, just to make my point clear.
if i prepare a syringe with a glass shed inside,
as stated above - then autoklave this syringe at 20 or even 25psi for
60mins,there wont be ANYTHING left bad and alive on it from my point of undestandment.

even, if the normal pressure cooker, running at 15psi,
the same syringe with the same glasshed, will be sterile if pc'd for 60mins.
prolly even shorter.

anyway, u get my point.
i know, that for example, endospores on rye, can survive pcing if the
rye isnt presoaked.

does the same factor apply to whatever may be on the syringe/glass shed
and does this actually MATTER for hobbiest?
peace ohm :mushroom2:


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InvisibleZen Peddler
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Re: Breaking up clumps of spores in syringe [Re: Zaphoid]
    #3516180 - 12/18/04 02:29 AM (20 years, 1 month ago)

this issue is so not advanced...


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Invisiblemicro
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Re: Breaking up clumps of spores in syringe [Re: Zen Peddler]
    #3519326 - 12/19/04 01:07 AM (20 years, 1 month ago)

Buy a vortex. They go for like $20 on labx.xom


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InvisibleHippie3
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Re: Breaking up clumps of spores in syringe [Re: Zen Peddler]
    #3556442 - 12/29/04 07:35 AM (20 years, 22 days ago)

Quote:

bluemeanie said:
this issue is so not advanced...




lol
i think perhaps
you just aren't 'advanced' enough in your thinking
to recognize potential when it's right in front of you.
but you're not alone in that.

kinda funny how just about everytime
something new & exciting comes along
lots of nay-sayers try to kill it fast.

first they delete the thread making the announcement,
so i tack my results on to this old thread.
suddenly a thread which had been in Advanced for almost EIGHT months
is no longer 'suitable'
and so they kick it down to here
where they hope fewer people will see it.
why ?
because it pains them to no end
that they didn't think of it,
but i did.

now here's some more
photo proof of just WHY
this ultrasonic method
is far better than anything we've used before.

from  ultrasonics for spore/tissue suspension?

Troutlips sez-
Quote:

made up 12, half pint PF jars.I inoculated three groups of four jars with different syringes.
The first group was a commercial syringe I bought last March of Nepal Chitwan, the second was from Karo water ,Texans, the third was a syringe I made from a print I made by placing the cap in a dry jar for 24 hrs., added water to the print and sat the jar on my ultrasonic humidifier for about an hour.These were B+.
On the fourth day the ultrasonic group were showing impressive growth while the other two groups showed little or no visible growth.





jars now 8 days old

as anyone [even those not-very-advanced] can plainly see,
the syringe exposed to the ultrasound
is producing extraordinary fast growth
compared to ordinary spore syringes
and even faster
than liquid culture [karo],
which supposedly is fastest of all.
:thumbup:
:cool:


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Edited by Hippie3 (12/30/04 03:53 AM)

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Offlineticktock
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
    #3556756 - 12/29/04 09:54 AM (20 years, 22 days ago)

To break up clumps in a syringe;
Heat needle to red in flame of alcohol lamp.
With the tip still in the flame, draw 1 ml of hot air.
The air space in the barrel allows some serious sloshing.
Works for me.


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InvisibleHippie3
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Re: Breaking up clumps of spores in syringe [Re: ticktock]
    #3560331 - 12/30/04 03:26 AM (20 years, 21 days ago)

another nice tip,
but this sonic method is still different
and i think 'better'
as no air is drawn in
hence less risk of introducing contams
but to each his own.


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Edited by Hippie3 (12/30/04 03:52 AM)

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Invisibleboxtop703
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
    #3560373 - 12/30/04 03:55 AM (20 years, 21 days ago)

Quote:

Hippie3 said:
does that give you
nearly full colonization
in just 4 days ?
don't think so...




This looks valid and I'm going to begin incorporating this into the TLG syringe making process I do believe.

For more thoroughly dispersed microscopy samples of course.

It is very simple but who said an idea had to be complicated to be effective so while it may or may not be "advanced", it most certainly appears to be an advancement.

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OfflineAnnoA
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
    #3560835 - 12/30/04 09:57 AM (20 years, 21 days ago)

Quote:

The first group was a commercial syringe I bought last March of Nepal Chitwan, the second was from Karo water ,Texans, the third was a syringe I made from a print I made by placing the cap in a dry jar for 24 hrs.




While I don't doubt(and know from my own experience) that there is a benefit in dispersing the spores well, this particular experiment is comparing apples to oranges.

If you want to see the true benefit of a good spore dispersal, you have(or he has) to prepare the syringes from the same spore print, and give some a ultrasonic bath, some a prolonged shaking(with an air bubble in the syringe) and some nothing.
Then you will see a difference, which, as I am willing to bet, will be visible, but not at all as dramatic as in the above example.

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Offlinescatmanrav
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Re: Breaking up clumps of spores in syringe [Re: Anno]
    #3561043 - 12/30/04 11:13 AM (20 years, 21 days ago)

Quote:

Anno said:
Quote:

The first group was a commercial syringe I bought last March of Nepal Chitwan, the second was from Karo water ,Texans, the third was a syringe I made from a print I made by placing the cap in a dry jar for 24 hrs.




While I don't doubt(and know from my own experience) that there is a benefit in dispersing the spores well, this particular experiment is comparing apples to oranges.

If you want to see the true benefit of a good spore dispersal, you have(or he has) to prepare the syringes from the same spore print, and give some a ultrasonic bath, some a prolonged shaking(with an air bubble in the syringe) and some nothing.
Then you will see a difference, which, as I am willing to bet, will be visible, but not at all as dramatic as in the above example.




Exactly what I was going to say. I'm gratful for the info and will end up with one of these (especially for liquid cultures..sounds very interesting) but I think a better experiment should be done...at least a control (unshaken, same spores) should have been used.


--------------------
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Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
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Offlinetroutlips
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Re: Breaking up clumps of spores in syringe [Re: Anno]
    #3562972 - 12/30/04 08:32 PM (20 years, 21 days ago)

You are right, Anno. I was not really trying to prove anything with that batch of three different strains,but when I noticed the result, I posted it in a similar thread to this one at 'Topia.
Hippie just thought that it demonstrated the ultra sonic dispersal tek pretty well and posted it here.
I was just trying it out for myself,not by any means a scientific experiment. I was just tryin' to grow some shrooms!
But you are right. Next batch I build, I will do as you suggest and use the same print prepared in the three methods you mentioned above.
Peace.

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Offlinedjred
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Re: Breaking up clumps of spores in syringe [Re: troutlips]
    #3563852 - 12/31/04 12:06 AM (20 years, 21 days ago)

i read this whole thread..well most of what seemed of use..still tick tock is the only one who i found to give results..somehow it wen tfomr syringes to ultra sonic jewlery cleaner..to somehting else..tlg is the bomb i still haven't broke up that syringe like it was the day i got it..hopefully i broke it up enough..it was 1 huge clump until i beat the shit out of it for 30 minutes..

thinking enough spores got jarred loose for success hopefully..?even though some clumps hang around?

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InvisibleHippie3
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Re: Breaking up clumps of spores in syringe [Re: Anno]
    #3583037 - 01/05/05 05:22 AM (20 years, 15 days ago)

Quote:

Anno said:
Quote:

The first group was a commercial syringe I bought last March of Nepal Chitwan, the second was from Karo water ,Texans, the third was a syringe I made from a print I made by placing the cap in a dry jar for 24 hrs.




While I don't doubt(and know from my own experience) that there is a benefit in dispersing the spores well, this particular experiment is comparing apples to oranges.

If you want to see the true benefit of a good spore dispersal, you have(or he has) to prepare the syringes from the same spore print, and give some a ultrasonic bath, some a prolonged shaking(with an air bubble in the syringe) and some nothing.
Then you will see a difference, which, as I am willing to bet, will be visible, but not at all as dramatic as in the above example.




people do this work
in real life,
with what they have on hand.
while it might not meet all rigorous requirements of
a truly 'scientific' study
nevertheless
anyone can see the very obvious difference in growth.
that is a valid observation
even if the methodology is flawed.
now find something else to criticize, nit-pick, etc.
if one doesn't create
i guess all that's left
is to destroy.
have fun.


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OfflineAnnoA
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
    #3583046 - 01/05/05 05:28 AM (20 years, 15 days ago)

>anyone can see the very obvious difference in growth.
>that is a valid observation
>even if the methodology is flawed.

Sure there is a difference in growth.

But to draw the conclusions you draw from it, is more than flawed.
I am glad to see everybody (but you) realizes this.

>now find something else to criticize, nit-pick, etc.

Keep your hostile attitude at mycotopia, thank you.

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Invisibleohmatic
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
    #3583048 - 01/05/05 05:30 AM (20 years, 15 days ago)

well i stick to sterilizing my syringes with a little glass shed inside
which is a super handy helper once the syringes are prepared since it breaks
apart spores in no time.

no need for all this fancy super duper stuff :wink:
even the standard air bubble in the syring helps, the shed is even better.
peace ohm :mushroom2:


--------------------
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RIP #cultivation! ....can't associate? well FUCK U !

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Offlinescatmanrav
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Re: Breaking up clumps of spores in syringe [Re: ohmatic]
    #3584624 - 01/05/05 03:10 PM (20 years, 15 days ago)

You put a whole shed in your syringe? And where do you get glass sheds? I've seen wooden ones and metal ones but I dont think I've ever seen a glass shed. It wouldnt be very good for keeping people away from your tools or lawnmower or anything. Plus I still think it would be difficult to put a whole shed into any of the syringes I have.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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OfflineAnnoA
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Re: Breaking up clumps of spores in syringe [Re: scatmanrav]
    #3588096 - 01/06/05 08:26 AM (20 years, 14 days ago)

It is difficult, but it is possible :wink:

Well, here are the images from a test done at mycotopia like it is supposed to be done.
Syringes prepared from the same  spore print , one syringe treated with ultrasonic, one not.

Would you call  this  a "very obvious difference" in growth? Or even  "dramatic" ?




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OfflineAnnoA
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Re: Breaking up clumps of spores in syringe [Re: scatmanrav]
    #3588107 - 01/06/05 08:30 AM (20 years, 14 days ago)

Quote:

scatmanrav said:
And where do you get glass sheds?




You find them in the woods, of course!


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Invisibleohmatic
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Re: Breaking up clumps of spores in syringe [Re: Anno]
    #3588164 - 01/06/05 08:44 AM (20 years, 14 days ago)

Quote:

Anno said:
Quote:

scatmanrav said:
And where do you get glass sheds?




You find them in the woods, of course!






:rotfl: :rotfl: :rotfl:
gotta love anno's humor :laugh:

peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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InvisiblePeterthinks
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Re: Breaking up clumps of spores in syringe [Re: Anno]
    #3588199 - 01/06/05 08:52 AM (20 years, 14 days ago)

Cool.
:jester:
couldn't you just expell the whole syringe into a sterile container and draw it back inside to break up the clumps?
And as for liquid cultures growing in ultrasound...try sticking your finger in an ultrasound bath!Would you feel like breeding in there?
LOL sorry I don't mean to laugh but it's a favorite trick of mine to play on people"You gotta feel this,it's so wierd!"
I have an ultrasonic mist maker that I use for certain things(ahem!)
I will be adding "spore shaker"to the list though,nice pics,I'm convinced.
I make prints on strips of spawnbag then throw them right into the syringe.

This will really shake them loose!

Thanks!


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Offlinescatmanrav
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Re: Breaking up clumps of spores in syringe [Re: Anno]
    #3612958 - 01/11/05 01:41 PM (20 years, 9 days ago)

Quote:

Anno said:
Quote:

scatmanrav said:
And where do you get glass sheds?




You find them in the woods, of course!






Ok but now I'm stuck finding syringes that the shed will fit into...thats a pretty big shed too.....


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

Extras: Filter Print Post Top
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