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OfflinePADDOHEAD
Corvus coronecorone
Male
Registered: 04/01/00
Posts: 134
Loc: The Netherlands
Last seen: 16 years, 6 months
2 and a half year old spore syringe
    #3562033 - 12/30/04 03:46 PM (19 years, 3 months ago)

Hi all,

We (roommate and i) are anxious to start 2005 with a new project and were wondering if a 2 and 1/2 year old spore syringe still would do the trick or is it a waste of time? The syringes were stored in the dark at room temperature.

Thx in advance,


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Cannagrow

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Offlinepenlight438094
1 spliff 16.1g +1 man alone =Blitzed!

Registered: 10/01/04
Posts: 933
Last seen: 10 years, 5 months
Re: 2 and a half year old spore syringe [Re: PADDOHEAD]
    #3562042 - 12/30/04 03:50 PM (19 years, 3 months ago)

that seems a little old was it refridgerated


--------------------
Grown on Uncle Ben's 5 Minute Rice
Looks nice, Think again, read and see http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3576624/an/0/page/0


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OfflinePADDOHEAD
Corvus coronecorone
Male
Registered: 04/01/00
Posts: 134
Loc: The Netherlands
Last seen: 16 years, 6 months
Re: 2 and a half year old spore syringe [Re: penlight438094]
    #3562061 - 12/30/04 03:56 PM (19 years, 3 months ago)

Thx for the re, no the syringes were stored at room temperature (15 - 20 celcius)


--------------------
Cannagrow

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Offlinemudvayne
newbie

Registered: 08/19/04
Posts: 64
Last seen: 18 years, 9 months
Re: 2 and a half year old spore syringe [Re: penlight438094]
    #3562065 - 12/30/04 03:58 PM (19 years, 3 months ago)

spores last FOREVER. It's worth a shot...

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Offlinepenlight438094
1 spliff 16.1g +1 man alone =Blitzed!

Registered: 10/01/04
Posts: 933
Last seen: 10 years, 5 months
Re: 2 and a half year old spore syringe [Re: PADDOHEAD]
    #3562067 - 12/30/04 03:59 PM (19 years, 3 months ago)

humm... if they do work then the growth will be considerably slower than with fresh spores, thats if they do work at all, my advice is wait for a few more responses


--------------------
Grown on Uncle Ben's 5 Minute Rice
Looks nice, Think again, read and see http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3576624/an/0/page/0


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Offlinemudvayne
newbie

Registered: 08/19/04
Posts: 64
Last seen: 18 years, 9 months
Re: 2 and a half year old spore syringe [Re: penlight438094]
    #3562077 - 12/30/04 04:01 PM (19 years, 3 months ago)

if they work they will work the same as any other spore syringe

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Offlinepenlight438094
1 spliff 16.1g +1 man alone =Blitzed!

Registered: 10/01/04
Posts: 933
Last seen: 10 years, 5 months
Re: 2 and a half year old spore syringe [Re: mudvayne]
    #3562079 - 12/30/04 04:02 PM (19 years, 3 months ago)

ya but slower


--------------------
Grown on Uncle Ben's 5 Minute Rice
Looks nice, Think again, read and see http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3576624/an/0/page/0


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Offlinemudvayne
newbie

Registered: 08/19/04
Posts: 64
Last seen: 18 years, 9 months
Re: 2 and a half year old spore syringe [Re: mudvayne]
    #3562090 - 12/30/04 04:05 PM (19 years, 3 months ago)

I don't know about slower, I used a syringe that was a year old before and it didn't colonize any slower than ones that I bought from the shroomery vendors. Maybe that's just me though...

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Offlinepenlight438094
1 spliff 16.1g +1 man alone =Blitzed!

Registered: 10/01/04
Posts: 933
Last seen: 10 years, 5 months
Re: 2 and a half year old spore syringe [Re: mudvayne]
    #3562097 - 12/30/04 04:07 PM (19 years, 3 months ago)

if thats the case the only problem should be wreather or not the spores are still viable, I know a print will last forever but will a syringe?? I say go for it, what the hell??? it's worth a shot


--------------------
Grown on Uncle Ben's 5 Minute Rice
Looks nice, Think again, read and see http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3576624/an/0/page/0


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InvisibleEonTan
bird
 User Gallery
Registered: 08/18/04
Posts: 468
Loc: very south
Re: 2 and a half year old spore syringe [Re: PADDOHEAD]
    #3562117 - 12/30/04 04:16 PM (19 years, 3 months ago)

They should work fine. Probably have little clumps of mycelium already in the syringe.

I wouldn't let that stop me from maybe getting something New that has come out since your mini retirement. :smile:

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Offlinepenlight438094
1 spliff 16.1g +1 man alone =Blitzed!

Registered: 10/01/04
Posts: 933
Last seen: 10 years, 5 months
Re: 2 and a half year old spore syringe [Re: EonTan]
    #3562127 - 12/30/04 04:21 PM (19 years, 3 months ago)

true, true, there are some nice new strains out, cheap too, i'd look into that personally


--------------------
Grown on Uncle Ben's 5 Minute Rice
Looks nice, Think again, read and see http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3576624/an/0/page/0


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Offlinemudvayne
newbie

Registered: 08/19/04
Posts: 64
Last seen: 18 years, 9 months
Re: 2 and a half year old spore syringe [Re: penlight438094]
    #3562235 - 12/30/04 04:54 PM (19 years, 3 months ago)

clumps of myc. are the best, then you don't have to wait around for a week for germination to take place  :thumbup:

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OfflineMushroomFriend
I smell a conspiracy!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 21 days, 5 hours
Re: 2 and a half year old spore syringe [Re: mudvayne]
    #3562240 - 12/30/04 04:56 PM (19 years, 3 months ago)

lol, without clumps germ starts at 2 or 3 days here after inoc with a viable syringe:p

1 clump and all your spores are at one place, no good.


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Offlinemudvayne
newbie

Registered: 08/19/04
Posts: 64
Last seen: 18 years, 9 months
Re: 2 and a half year old spore syringe [Re: MushroomFriend]
    #3562276 - 12/30/04 05:09 PM (19 years, 3 months ago)

damn I'm always waiting around 1 to 2 weeks before I see anything...unless there are already clumps in the syringe. I definitely gotta look into that karo tek.

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OfflinePADDOHEAD
Corvus coronecorone
Male
Registered: 04/01/00
Posts: 134
Loc: The Netherlands
Last seen: 16 years, 6 months
Re: 2 and a half year old spore syringe [Re: mudvayne]
    #3564274 - 12/31/04 03:50 AM (19 years, 2 months ago)

Thx for the replies.

There are no clumps in em.

Ill surely have a look in to the new more 'exotic' strains available then just these Ecuador :smile:
Any tips?

Peace,


--------------------
Cannagrow

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Offlinemudvayne
newbie

Registered: 08/19/04
Posts: 64
Last seen: 18 years, 9 months
Re: 2 and a half year old spore syringe [Re: PADDOHEAD]
    #3564341 - 12/31/04 05:34 AM (19 years, 2 months ago)

there are so many strains out there it's all a matter of personal preference. some are fast colonizers, some are slow, some produce huge shrooms, others produce small. My favorite is the cubensis blue meanies strain. They look cool and I got 4 nice flushes from a casing. Good luck.

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OfflineTantalus
Beyonddescription.
Registered: 06/05/03
Posts: 747
Last seen: 18 years, 6 months
Re: 2 and a half year old spore syringe [Re: PADDOHEAD]
    #3565216 - 12/31/04 12:04 PM (19 years, 2 months ago)

In this case it's better that they don't have clumps of myc. After that long it would be dead. Spores will last forever, but myc won't.

I'd say go for it, if you've kept them clean, their age really shouldn't be a problem.


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"A nation's hope of lasting peace cannot be firmly based upon any race in armaments but rather upon just relations and honest understanding with all other nations...

Every gun that is made, every warship launched, every rocket fired signifies, in the final sense, a theft from those who hunger and are not fed, those who are cold and are not clothed... The cost of one modern heavy bomber is this: a modern brick school in more than 30 cities. We pay for a single fighter with a half million bushels of wheat. We pay for a single destroyer with new homes that could have housed more than 8,000 people..."

President Dwight Eisenhower, 1953

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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 2 months
Trusted Cultivator
Re: 2 and a half year old spore syringe [Re: Tantalus]
    #3567038 - 12/31/04 11:19 PM (19 years, 2 months ago)

Your Ecuadors are a favorite strain among many...give them a shot. Strains will grow similarly ad youll need to try some out and find your favorites but since you have eq..start there..you may find its all you need. Tanatalus is correct about the absense of myc being a good thing at this point..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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InvisibleEonTan
bird
 User Gallery
Registered: 08/18/04
Posts: 468
Loc: very south
Re: 2 and a half year old spore syringe [Re: scatmanrav]
    #3567994 - 01/01/05 11:00 AM (19 years, 2 months ago)

Mycelium does survive storage in sterile water for long periods of time.

Spores germinate in water, and the resulting mycelium does stay alive for at least 4 years. Probably alot longer then that, but I'll go with what I have Experience with versus what I estimate.

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OfflinePADDOHEAD
Corvus coronecorone
Male
Registered: 04/01/00
Posts: 134
Loc: The Netherlands
Last seen: 16 years, 6 months
Re: 2 and a half year old spore syringe [Re: scatmanrav]
    #3568000 - 01/01/05 11:07 AM (19 years, 2 months ago)

Thx for the input people its much appreciated, ill try and get things started next week.

Saw a 6 liter pressurecooker for 29 euro, get me some substrate and we'll see what happens :smile:

Best wishes for 2005!


--------------------
Cannagrow

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OfflinePADDOHEAD
Corvus coronecorone
Male
Registered: 04/01/00
Posts: 134
Loc: The Netherlands
Last seen: 16 years, 6 months
Re: 2 and a half year old spore syringe [Re: PADDOHEAD]
    #3657808 - 01/21/05 09:14 AM (19 years, 2 months ago)

They still work....

We have some mycelium growing in 3 of the 4 jars with rye, sadly we had to get rid of 2 of the best colonized ones because of blue contams..(after six days).

We used our school lab with a flowhood setup for inocculating and a stove set at 25 celcius for colonizing.
Could these blues be some kind of endospore contam?
The rye was pressurecooked for about 1 hour at max setting, but we did not keep the rye in water before setting up the cooker.
Do u think thats the mistake we made...?

We'll try again next week with the remaining spore solution.

Peace,


--------------------
Cannagrow

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InvisibleHolydiver
Stranger
 User Gallery

Folding@home Statistics
Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea
Trusted Cultivator
Re: 2 and a half year old spore syringe [Re: PADDOHEAD]
    #3657910 - 01/21/05 09:56 AM (19 years, 2 months ago)

Quote:

PADDOHEAD said:
we did not keep the rye in water before setting up the cooker.
Do u think thats the mistake we made...?





It's very likely  :frown:


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To find a place to live between the negatives and positives.

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OfflinePADDOHEAD
Corvus coronecorone
Male
Registered: 04/01/00
Posts: 134
Loc: The Netherlands
Last seen: 16 years, 6 months
Re: 2 and a half year old spore syringe [Re: Holydiver]
    #3658158 - 01/21/05 11:42 AM (19 years, 2 months ago)

How long should u pre-soak the rye to germinate these endospores?
Should 24 hours be sufficient?

Thx,


--------------------
Cannagrow

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