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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Rye Grain, Wild Bird Seed

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InvisibleColonel Kurtz Ph.D
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YOUR ratio of viable/Contame'd cakes?
    #3547230 - 12/27/04 08:56 AM (11 years, 11 months ago)

I'm interested in getting to know wich kind of contamination ratios are to be expected with any kind of tek, for I'm looking to improve my sterile technique. Also I hope this helps more people to reduce their contam ratios by sharing our best teks.

So, have you always gotten some contams with PF and syringe but none with Agar and spore prints? Let the shroomery know! :wink: Maybe I should make a poll?

:mushroom2: :mushroom2: :mushroom2:


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OfflineMAIA
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Re: YOUR ratio of viable/Contame'd cakes? [Re: Colonel Kurtz Ph.D]
    #3548174 - 12/27/04 03:11 PM (11 years, 11 months ago)

I think we don't compile that kind of information (statistics).
Anyway, try making a poll and i'll sticky it. It looks like an interesting idea....

MAIA


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Offlinepenlight438094
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Re: YOUR ratio of viable/Contame'd cakes? [Re: MAIA]
    #3548482 - 12/27/04 04:52 PM (11 years, 11 months ago)

pf- tek i get 2 out of 12 contam rate
wbs 5 out of 10
rye 2 out of 10
pf tek with brown rye instead of brf 1 out of 12
just from my latest batches, cant remember beyond that, i once got a contamed syringe and lost all 15 jars I innoced


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Grown on Uncle Ben's 5 Minute Rice
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InvisibleHeineken
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Re: YOUR ratio of viable/Contame'd cakes? [Re: penlight438094]
    #3549735 - 12/27/04 09:07 PM (11 years, 11 months ago)

that would definetly suck. i'd shit a brick.


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Offlinepenlight438094
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Re: YOUR ratio of viable/Contame'd cakes? [Re: Heineken]
    #3550476 - 12/27/04 11:12 PM (11 years, 11 months ago)

I did shit a brick, then i had to swallow it again when i rembered i forgot to re-starilize the syringe


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Grown on Uncle Ben's 5 Minute Rice
Looks nice, Think again, read and see http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3576624/an/0/page/0



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OfflinePorchMonkey321
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Re: YOUR ratio of viable/Contame'd cakes? [Re: Colonel Kurtz Ph.D]
    #3551530 - 12/28/04 03:28 AM (11 years, 11 months ago)

My last grow went badly, lost 7 out of 9 pints, then lost the casing of the last two jars. Remedied the problem though; built myself a glovebox!


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Offlinepenlight438094
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Re: YOUR ratio of viable/Contame'd cakes? [Re: PorchMonkey321]
    #3552195 - 12/28/04 12:55 PM (11 years, 11 months ago)

its always the failure that makes us take the next step


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Grown on Uncle Ben's 5 Minute Rice
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InvisibleColonel Kurtz Ph.D
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Re: YOUR ratio of viable/Contame'd cakes? [Re: penlight438094]
    #3580450 - 01/04/05 06:48 PM (11 years, 10 months ago)

Wow, I forgot completely about this topic, sorry! Well, I'll post some info on my ratios... see if I can make that poll, 'coz I really don't know how I could make this more scientific now that I think about it :frown:

-Popcorn tek
15 x 1000 cc micro-boxes
PC'd 45-60 minutes, Inoc'd 1 to 2 days after.
Homemade Glovebox
Disinfectant: Rubbing alcohol
Tested syringes, 4 strains, and  non-tested myc culture
  Results: 2 Good , 13 contam'd.

-PF tek
12 x 1000 cc micro-boxes
PC'd 25-60 minutes, Inoc'd 1 to 2 days after.
Homemade Glovebox
Disinfectant: Bleach (10:1 water/bleach ratio), alcohol and flame for needles
Tested syringes, 4 strains, some syringes may be a bit too old.
  Results: 6 Good, 6 not showing myc after 8 days, no contamns.

What I see here is that in my experience, sterilization time does not seem to matter as much as the used disinfectant for the needles and glovebox.

Also it seems to me that myc culture is harder to keep away from contams, at least in honey water, and that grains are way more prone to bacterial contamination than BRF, which indeed makes it more appropriate for beginners such as myself.

The use of micro-boxes apparently excludes handling and incubation ambient as a contamination factor.

Anyway, that's it for me so long...


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