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Offlineinfamous
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CC's per jar
    #3539687 - 12/24/04 05:19 PM (12 years, 6 months ago)

Im about to inoculate jars for the first time, I have 10cc syringes, How many cc's should I use per jar. Im going to inoculate 2 pint jars (ran out of 1/2 pints) with Blue meanies and im wanted to inoculate 4 1/2pint jars with Golden Teacher. However I dont know if I have enough spores. Any Info on the correct amount of CC's to use or experiance with either of these two strains would be appreciated.


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OfflineGreen_Snake
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Re: CC's per jar [Re: infamous]
    #3539698 - 12/24/04 05:22 PM (12 years, 6 months ago)

a 10 cc syringe can be used for at least 20 1/2pint jars IME&O

at least for the cubensis I havent grown blue meanies yet but i assume its the same.


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Offlineinfamous
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Re: CC's per jar [Re: Green_Snake]
    #3539700 - 12/24/04 05:25 PM (12 years, 6 months ago)

I was also wondering I read on one tek to use moist vermiculte in your terrarium but i read another tek that says you dont want your cake to be in contact with moisture and u should build a screen to raise your cake off of the moist floor of the terrarium, Wich is the correct way???


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here I stand head in hand turn my face to the wall


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OfflineGreen_Snake
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Re: CC's per jar [Re: infamous]
    #3539702 - 12/24/04 05:27 PM (12 years, 6 months ago)

-i have my pf cakes on wet perlitte. I use a layer of dry verm to protect the jars from air contams and this dry verm is also used as bottom for the cake so its not in direct contact with perlitte. A screen will probably work just as good if not better so I would go for that


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Offlinexxduffxx
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Re: CC's per jar [Re: infamous]
    #3539705 - 12/24/04 05:28 PM (12 years, 6 months ago)

question

when you inoculate jars and put them in the terrarium you want no HF.
right?


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Invisiblewoodzmun
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Re: CC's per jar [Re: infamous]
    #3539706 - 12/24/04 05:29 PM (12 years, 6 months ago)

I would use perlite, not vermiculite in a terranium. Perlite wicks moisture whereas verm. hold it in. I would find something to set the cakes on or they will waste energy trying to colonize the perlite there sitting on. Don't forget to dunk them overnite though to make sure there hydrated. GL.


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Invisiblewoodzmun
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Re: CC's per jar [Re: woodzmun]
    #3539722 - 12/24/04 05:39 PM (12 years, 6 months ago)

A question duff what is HF? I would put inoculated jars in a incubator at about 82 to 85 degrees F preferably in the dark.


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OfflineMushroomFriend
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Re: CC's per jar [Re: woodzmun]
    #3539728 - 12/24/04 05:41 PM (12 years, 6 months ago)

SUPER AVATAR WOODZzz! :thumbup:

(And i am not easy impressed! :wink: )


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Invisiblewoodzmun
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Re: CC's per jar [Re: MushroomFriend]
    #3539745 - 12/24/04 05:47 PM (12 years, 6 months ago)

Thanks MF, it is one of my favorite albums from my favorite band.


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OfflineClavicep
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Re: CC's per jar [Re: woodzmun]
    #3539788 - 12/24/04 05:59 PM (12 years, 6 months ago)

use perlite, for sure. 1cc a jar is ample.


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Offlineticktock
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Re: CC's per jar [Re: infamous]
    #3539899 - 12/24/04 06:34 PM (12 years, 6 months ago)

Quote:

infamous said:
However I don't know if I have enough spores.




If you can wait a few days, you have more than enough spores. 1 or 2 cc's of spore water can make 10 syringes of liquid mycelium culture.
You just need some karo or light honey. Here's the tek;
http://www.shroomery.org/index/par/8005


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OfflineMushroomFriend
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Re: CC's per jar [Re: infamous]
    #3540055 - 12/24/04 07:34 PM (12 years, 6 months ago)

Its one of my favourite birds! The holy owl! :laugh:


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Offlinedonkey_lipz
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Re: CC's per jar [Re: MushroomFriend]
    #3540074 - 12/24/04 07:43 PM (12 years, 6 months ago)

Is 1 CC of spore solution good enough for a quart grain jar?


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Offlinedmc_
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Re: CC's per jar [Re: donkey_lipz]
    #3540083 - 12/24/04 07:49 PM (12 years, 6 months ago)

Quote:

donkey_lipz said:
Is 1 CC of spore solution good enough for a quart grain jar?




1/8 of a CC is good enough for a guart grain jar. Some will use high amounts of inoculum to speed colonization and reduce chance of contams getting a foothold.

Personally I use 2CCs per jar and make about 5 jars then g2g. 1CC works great.


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Offlineticktock
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Re: CC's per jar [Re: donkey_lipz]
    #3540095 - 12/24/04 07:54 PM (12 years, 6 months ago)

Quote:

donkey_lipz said:
Is 1 CC of spore solution good enough for a quart grain jar?




I think so, if everything is sterile and you don't mind waiting longer for colonization. Your goal is to get the mycelium off and running, and overpowering any contaminants.

Then again, all syringes are not created equal. Your 1 cc might have more spores than my whole syringe. There's room for error in just about everything but cleanliness.


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OfflineMushroomFriend
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Re: CC's per jar [Re: infamous]
    #3540101 - 12/24/04 07:56 PM (12 years, 6 months ago)

Some say it will suffice.
But most say 2-2,5CC will be the standard..... Idd go with the last.

1CC must be possible but slower colonisation.


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Offlineinfamous
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Re: CC's per jar [Re: MushroomFriend]
    #3540286 - 12/24/04 09:23 PM (12 years, 6 months ago)

dunk? I havent read that anywhere yet. So im assuming when I take the cakes from the jars i dunk them in sterile water or let them sit overnight? Sorry I dont know if I missed that part in the tek.


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The land of the free on paper looks great!



here I stand head in hand turn my face to the wall


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Offlineticktock
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Re: CC's per jar [Re: infamous]
    #3540616 - 12/24/04 11:24 PM (12 years, 6 months ago)

A short version of dunk and roll because I can't find the tek just now.

Open the lid of your colonized PF jar and scrape all the top verm off with a clean fork. Fill the jar to the top with plain water or, (better), peroxide diluted to .3% Let the cake stand for 12 to 24 hours underwater. Many believe that this time should be spent in the fridge. You are getting as much water as you can into the cake, and cold shocking it.

Prepare a jar lid, bottom of a beer can, or similar base by putting some verm into it, soaked with the same thing your cakes are in.

Take the wet cake out of the jar and drop in into your bag of verm. Cover it with verm, then take it out. Don't let it lay in there very long or the verm will just pull the moisture away.

Stand the verm covered cake on the wet verm that you prepared. Put another heaping spoon of dry verm on top. Gently mist with the same juice as above to saturate.

What you end up with is a cake that's sort of like an almost casing. It's harder to read this than it is to actually do it.


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Offlinexxduffxx
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Re: CC's per jar [Re: ticktock]
    #3540652 - 12/24/04 11:32 PM (12 years, 6 months ago)

thats some good info. after you do this how long will it take to grow mushrooms? do you want to cover in verm every time you flush?


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Roger Out


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Offlineinfamous
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Re: CC's per jar [Re: ticktock]
    #3540657 - 12/24/04 11:34 PM (12 years, 6 months ago)

I understand, thanks alot for the help, I inoculated some jars tonight so im a lil far from dunk n rolling but im hoping all goes well. Again thanks for the help


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The land of the free on paper looks great!



here I stand head in hand turn my face to the wall


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