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Amazon Shop: ½ Pint Jars, Autoclave, Pressure Cooker

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OfflineHydroSmurf
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How do you sterilize a casing layer?
    #3539111 - 12/24/04 01:56 PM (12 years, 11 months ago)

The subject says it all...how do you sterilize a casing layer with a pressure cooker? No autoclaves around here... :mushroom2:


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I never supposed for the love of me that it would all be so vivid.


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OfflineKaptKid
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Re: How do you sterilize a casing layer? [Re: HydroSmurf]
    #3539131 - 12/24/04 01:59 PM (12 years, 11 months ago)

15psi for 60 min.


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Child of the 60's, Tripping ever since.


:sun:


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OfflineHydroSmurf
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Re: How do you sterilize a casing layer? [Re: KaptKid]
    #3539136 - 12/24/04 02:00 PM (12 years, 11 months ago)

I mean do you add water? In that case won't the casing layer get wet?


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I never supposed for the love of me that it would all be so vivid.


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OfflineKaptKid
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Re: How do you sterilize a casing layer? [Re: HydroSmurf]
    #3539169 - 12/24/04 02:08 PM (12 years, 11 months ago)

Make casing layer as per tek.Load into jars or bags.Depending on how much you need.Do foil just like pf tek on jars.Don't want to change water content.


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Child of the 60's, Tripping ever since.


:sun:


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InvisibleAlkaloids
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Re: How do you sterilize a casing layer? [Re: HydroSmurf]
    #3539273 - 12/24/04 02:34 PM (12 years, 11 months ago)

I would recommend pasteurization over sterilization for a casing layer.
The FAQs have lots of info about different forms of pasteurization, but one simply method is to hydrate your casing layer to field capacity. That is add water until it takes a firm squeeze to get water out. Not soggy and dripping wet, but also not so dry that you have to squeeze hard.

Then cover it with saran wrap or some other plastic wrap and microwave it for 5 minutes (in a 1000W microwave), stir, then microwave for an additional 5 minutes. On high power setting. This works quite well even when using larger amounts of casing. Success can be had with up to at least 500 gm casing material and 1.5 L of water.

good luck

make sure to let it cool enough before putting on the mycelium as thermal death limits for Ps. cubensis have been established at around 104 degrees Fahrenheit/ 40 C. So if it is any warmer than body temperature it is too warm.


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OfflineMycoJunkie
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Re: How do you sterilize a casing layer? [Re: Alkaloids]
    #3539434 - 12/24/04 03:18 PM (12 years, 11 months ago)

for 50/50+ you can nuke it in the microwave for 7 minutes.

http://www.shroomery.org/index/par/7977


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OfflineGreen_Snake
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Re: How do you sterilize a casing layer? [Re: MycoJunkie]
    #3539508 - 12/24/04 03:41 PM (12 years, 11 months ago)

I have always just poured boiling water over the casing materials. I do not think the materials needs to be sterilized UNLESS contams is a repeating problem on your casings.


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Invisibletahoe
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Re: How do you sterilize a casing layer? [Re: Green_Snake]
    #3539565 - 12/24/04 04:13 PM (12 years, 11 months ago)

nuke it


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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OfflineHydroSmurf
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Re: How do you sterilize a casing layer? [Re: tahoe]
    #3543743 - 12/26/04 07:11 AM (12 years, 11 months ago)

Thanks a bunch though. Think I'll do the microwave thingy. I love my microwave oven, lol.


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I never supposed for the love of me that it would all be so vivid.


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Offlinemudvayne
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Registered: 08/19/04
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Re: How do you sterilize a casing layer? [Re: HydroSmurf]
    #3543753 - 12/26/04 07:53 AM (12 years, 11 months ago)

wow, all this time I've only been microwaving my casing layers for 2 minutes - no contams yet (knock on wood)


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Amazon Shop: ½ Pint Jars, Autoclave, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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