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OfflineFarmerD
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Registered: 05/16/02
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mycelium injection
    #3536021 - 12/23/04 01:28 PM (19 years, 3 months ago)

okay i have an idea...I need a little help from the pros.
I have two jars that I accidentally put two lids on and boiled.......so there are no inoculation holes :frown:  My ? is could I prepare some syringe with water....and then use some mycelium off of a completed cake to inject into the new jars.........I mean Im gonna have to take the lid off anyway, so I kinda want to experiment is this possible to use already spawned mycelium?  And if so is there a certain teck to explain how I could do such a thing?
Thanks


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Offlinepenlight438094
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Registered: 10/01/04
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Re: mycelium injection [Re: FarmerD]
    #3536168 - 12/23/04 02:12 PM (19 years, 3 months ago)

This is pretty simple, get some sterile water

open a fully colonized cake, dump off the sealing verm layer and fill the jar(still contaning the cake) with the water

replace the lid and place in the fridge for 1 hour

shake the shit out of the jar untill it appears cloudy

using one of the original innoc. holes, draw the liquid into a sterile syringe, replace syringe cap and refridgerate (good for a few weeks)

allow jar to finish dunking for 12-24 hours more then birth as normal

for the jars you have to open, remove the lids and put only one back on, then re-PC, allow to cool and innoc with your mya-syringe, these jars will start showing signs of growth sooner than spores but take just as long to colonize


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Offlinelemon_lw
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Registered: 10/17/04
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Re: mycelium injection [Re: penlight438094]
    #3536290 - 12/23/04 02:46 PM (19 years, 3 months ago)

i know how you can use that jar. take off that lid. drill some holes in it and resterilize it. you can do as penlight says or look into karo. or grow mycelium from a mushie (ive never done this so im not to up on the technique)


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Offlinepenlight438094
1 spliff 16.1g +1 man alone =Blitzed!

Registered: 10/01/04
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Re: mycelium injection [Re: lemon_lw]
    #3536296 - 12/23/04 02:51 PM (19 years, 3 months ago)

I looked into that cloning method but found it was too dependant on absloute sterilility, personally I prefer the good old spore print and syringe, but thats just me


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Grown on Uncle Ben's 5 Minute Rice
Looks nice, Think again, read and see http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3576624/an/0/page/0


Edited by penlight438094 (12/23/04 02:52 PM)

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Offlinelemon_lw
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Re: mycelium injection [Re: penlight438094]
    #3536304 - 12/23/04 02:53 PM (19 years, 3 months ago)

yeah i dont know to much about it but i think the trick is to give the mushie a H2O2 soak before placing in jar. but yes you would have to have a glovebow for this work.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz

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InvisiblePawPaw
Picktish TexasStyle....

Registered: 12/20/04
Posts: 8,243
Loc: was born down in the sout...
Re: mycelium injection [Re: FarmerD]
    #3536687 - 12/23/04 04:53 PM (19 years, 3 months ago)

Take a corkscrew or a ice pick and punch some holes in the lids...


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