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FarmerD
journeyman


Registered: 05/16/02
Posts: 195
Loc: US OF A BABY!!!!!!
Last seen: 18 years, 4 months
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mycelium injection
#3536021 - 12/23/04 01:28 PM (19 years, 1 month ago) |
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okay i have an idea...I need a little help from the pros. I have two jars that I accidentally put two lids on and boiled.......so there are no inoculation holes My ? is could I prepare some syringe with water....and then use some mycelium off of a completed cake to inject into the new jars.........I mean Im gonna have to take the lid off anyway, so I kinda want to experiment is this possible to use already spawned mycelium? And if so is there a certain teck to explain how I could do such a thing? Thanks
-------------------- None of these statements are to be taken seriously. FarmerD is a fictional character, and so are the things he posts. "He who reaches for the stars often stumble on a simple straw." Blue Fairy Supplies for all your shrooming needs! Special thanks to MushroomMadness for great deals!
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penlight438094
1 spliff 16.1g +1 man alone =Blitzed!

Registered: 10/01/04
Posts: 933
Last seen: 10 years, 3 months
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Re: mycelium injection [Re: FarmerD]
#3536168 - 12/23/04 02:12 PM (19 years, 1 month ago) |
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This is pretty simple, get some sterile water
open a fully colonized cake, dump off the sealing verm layer and fill the jar(still contaning the cake) with the water
replace the lid and place in the fridge for 1 hour
shake the shit out of the jar untill it appears cloudy
using one of the original innoc. holes, draw the liquid into a sterile syringe, replace syringe cap and refridgerate (good for a few weeks)
allow jar to finish dunking for 12-24 hours more then birth as normal
for the jars you have to open, remove the lids and put only one back on, then re-PC, allow to cool and innoc with your mya-syringe, these jars will start showing signs of growth sooner than spores but take just as long to colonize
-------------------- Grown on Uncle Ben's 5 Minute Rice Looks nice, Think again, read and see http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3576624/an/0/page/0
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lemon_lw
Stranger

Registered: 10/17/04
Posts: 3,622
Loc: That Way
Last seen: 16 years, 4 months
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i know how you can use that jar. take off that lid. drill some holes in it and resterilize it. you can do as penlight says or look into karo. or grow mycelium from a mushie (ive never done this so im not to up on the technique)
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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penlight438094
1 spliff 16.1g +1 man alone =Blitzed!

Registered: 10/01/04
Posts: 933
Last seen: 10 years, 3 months
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Re: mycelium injection [Re: lemon_lw]
#3536296 - 12/23/04 02:51 PM (19 years, 1 month ago) |
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I looked into that cloning method but found it was too dependant on absloute sterilility, personally I prefer the good old spore print and syringe, but thats just me
-------------------- Grown on Uncle Ben's 5 Minute Rice Looks nice, Think again, read and see http://www.shroomery.org/forums/showflat.php/Cat/0/Number/3576624/an/0/page/0
Edited by penlight438094 (12/23/04 02:52 PM)
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lemon_lw
Stranger

Registered: 10/17/04
Posts: 3,622
Loc: That Way
Last seen: 16 years, 4 months
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yeah i dont know to much about it but i think the trick is to give the mushie a H2O2 soak before placing in jar. but yes you would have to have a glovebow for this work.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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PawPaw
Picktish TexasStyle....

Registered: 12/20/04
Posts: 8,243
Loc: was born down in the sout...
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Re: mycelium injection [Re: FarmerD]
#3536687 - 12/23/04 04:53 PM (19 years, 1 month ago) |
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Take a corkscrew or a ice pick and punch some holes in the lids...
-------------------- Come to Paw Paws place there will be no sleeping tonight
Eric just wants to catch reds .. Don?t look back, don?t look back He?s right on your trail Don?t look back, don?t look back He?s just a step away from hell WDYWFM?
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