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OfflineFaaip_De_Oiad
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heavy inoculation
    #3532885 - 12/22/04 08:35 PM (12 years, 2 months ago)

when I knocked up my jars I used a 12 CC and going by the MMGG I had a little left over, so I squirted it into my last jar and marked the jar to see if it colonized faster.

Well it's been 12 days now, and that jar is at about 90% and the others aren't near as close.

so here's my question, I plan on becoming self reliant and making my own syringes in the future, so using extra spore solution won't be a problem, but are there any disavantages to doing this?


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InvisibleHeineken
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Re: heavy inoculation [Re: Faaip_De_Oiad]
    #3533025 - 12/22/04 09:10 PM (12 years, 2 months ago)

thats interesting i thought at the max you coulkd only put 2 cc in each hole


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Offlineincubaby_421
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Re: heavy inoculation [Re: Heineken]
    #3533168 - 12/22/04 09:49 PM (12 years, 2 months ago)

well evidently not


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Offlinedmc_
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Re: heavy inoculation [Re: incubaby_421]
    #3533186 - 12/22/04 09:53 PM (12 years, 2 months ago)

No harm will result from innoculating your media with a large amount of spores. However if a lot of water was used to transfer the spores it could oversaturate your media and cause problems.


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OfflineFaaip_De_Oiad
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Re: heavy inoculation [Re: dmc_]
    #3533470 - 12/22/04 11:08 PM (12 years, 2 months ago)

what kind of problems exactly, and how much is too much?

also, to make sure i've got this straight, when I make my own, I should just put more spores into my syringe (making a dark syringe instead of a clear one) as opposed to squirting more spore solution?


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Offlinedmc_
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Re: heavy inoculation [Re: Faaip_De_Oiad]
    #3533537 - 12/22/04 11:21 PM (12 years, 2 months ago)

Quote:

what kind of problems exactly



The mycelium not to colonize at a normal pace, if at all.

Quote:

and how much is too much?



sounds like an experiment!

Quote:

also, to make sure i've got this straight, when I make my own, I should just put more spores into my syringe (making a dark syringe instead of a clear one) as opposed to squirting more spore solution?



Ideally, yes. Water acts as a carrier, which is also a good thing. You will need to find a practical ratio and optimal amounts for inoculation. Experiment and let us know?


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