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Baby_Hitler
Errorist
Registered: 03/06/02
Posts: 27,625
Loc: To the limit!
Last seen: 9 hours, 11 minutes
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"Fungus obtained by the fusion of Grifola umbelleta and Ganoderma lucidum "
#3529562 - 12/21/04 10:16 PM (19 years, 2 months ago) |
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Is this what they call a "Chimera"?
http://patft.uspto.gov/netacgi/nph-Parse...mp;RS=ganoderma
A microorganism exhibiting excellent antibiotic and sterilizing properties for the preparation of medicines and the like, obtained by fusion of cells of two different fungal species. A Ganoderma and a Grifola, each exhibiting little or no antibiotic activity, are isolated and hybridized, and milled to produce a fused cell body. The cell body is black-brown, and has been deposited as deposit No. 3131 (FERM BP-3131).
1) Morphology
This is a mushroom that has a pileus and a stem that is lustrous, as though varnished with lacquer (when steamed). The pileus is a kidney type. Its surface is covered with a shell. Its color is a reddish brown and/or a black violet. Its pulp is corky, and consists of two layers. The upper layer is white. The portion near the spores is light cinnamon color, and the layer length of the tube is 5-10 mm. The tube hole is round, and there are 3 to 4 1 mm holes. The spore is an egg type; its membrane is dual in structure, the outer membrane being nearly colorless, the inner membrane having a weak brown small projection. Said projection is inserted from the inside to the outside. Its dimension is 9 to 11.times.5.5.times.7.mu.. Said shell wraps the pileus, and the stem has a thickness of 30-40.mu.. A brown cell of a thick club type membrane is arranged on said shell. A varnish-like material is secreted thereon.
2) Separation and Cultivation
Yellow tissue is picked from the ripe tip of the Ganoderma fruiting body, sterilely cut to a size of 3 mm.sup.3, inoculated onto an onion, soy sauce, and sesame oil agar culture medium, and cultured at a temperature of 25.degree. C. to 30.degree. C. for germination, resulting in propagation of the first white cotton wool hypha within 7 days. Such germinated hyphae are utilized in fusions with other fungi.
Said fungus can also be propagated on culture media of any composition.
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Tweexican
Clit Commander
Registered: 11/06/03
Posts: 657
Last seen: 5 years, 8 months
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Re: "Fungus obtained by the fusion of Grifola umbelleta and Ganoderma lucidum " [Re: Baby_Hitler]
#3530232 - 12/22/04 12:53 AM (19 years, 2 months ago) |
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that is crazy. It seems to have medicinal properties; "increased urine production"?
A mushroom born of fusion that flushes out your system. Wow.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 9 days, 20 hours
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Re: "Fungus obtained by the fusion of Grifola umbelleta and Ganoderma lucidum " [Re: Baby_Hitler]
#3530687 - 12/22/04 06:39 AM (19 years, 2 months ago) |
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EonTan
bird
Registered: 08/18/04
Posts: 468
Loc: very south
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Re: "Fungus obtained by the fusion of Grifola umbelleta and Ganoderma lucidum " [Re: Baby_Hitler]
#3532786 - 12/22/04 06:13 PM (19 years, 2 months ago) |
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No.
Chimera mutations are common in plants, and are vegetatively propagated via meristem culture(direct morphogenesis) growth arrising from groups of cells both mutated and non mutated, resulting in plants with both cell types present. VARIGATED LEAVES are chimeras.
If you attempt to vegetatively propagate these plants from single cells, you will get some plants with only green leaves, and some with only yellow leaves. The latter will not survive exvitro, it has no chlorophyll to manufacture energy to grow. If you vegetativly propagate using MERISTEM culture, groups of cells both mutated and non-mutated get cloned together and the chimera is maintained.
If the same thing was imagined into fungi, you would have a dikaryon wiht both mutated and non mutated cells present in a single dikaryon, both independently replicating themselves, but living in a single colony. The thought of Multispore orgies of substrains all clamping together via anastamosis, comes to mind, but these connections eventually work themselves out into seperate colonies, or into a single colony of like cells via rewiring of rogue substrains. MAJORITY RULE so to speak.
Both would have extreme limitations with culturing in fungi. You would have to be able to transfer goups of cells containing both the mutants and the non mutants.
The above interspecific hybrid is a single cell containing both a nuclei from one species and a nuceli from another. Each cell would be able to produce other inerspecific cells. A chimera is a group of cells, some cells within the group are mutated, and some are not.
Plant chimeras happen all on their own in nature. WE just clone them via meristem propagation, CLONING(cuttings/microcuttings).
If a single cell mutates in a fungi, that cell could lead to a new substrain colony growing entirely from that single cell. This is not a chimera.
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