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Amazon Shop: ½ Pint Jars, Agar

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OfflineMajestic
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Loc: Florence, Oregon
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A 'lot' of Karo questions!
    #3526490 - 12/21/04 03:58 AM (12 years, 8 months ago)

1) Its been a week and no signs of growth in karo. It seems all the spores somehow found there way to each other and have all clumped in one mass...so I spose there might be tiny germination, but has anyone else had this happen before? this happened 2 days after, and after i swirled to break it apart it happens again.

2)I have some honey culture with some mycelium going, but I believe it may be contaminated. Would the best way to isolate a contam free sample be to start a selection of agar dishes and pick one/all that doesnt contam?

3)What's everyones opinion on Karo? which liquid culture mix do u prefer?

:laugh: ~D


--------------------
"The Oracle at Delphi is quite possibly real...
I watched a documentary on it on Discovery channel."  -Darcho

People will always be tempted to wipe their feet,
on anything with 'welcome' written on it.

:regularshroom:?:regularshroom: "mushroom, what?"
_\ -/_


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Offlineliveby
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Re: A 'lot' of Karo questions! [Re: Majestic]
    #3526513 - 12/21/04 04:10 AM (12 years, 8 months ago)

1, search

2, its hard to tell with karo

3 , karo fast but not good for signs of contams


--------------------

http://www.bruceeisner.com/ -Creating a Sensible Culture


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Offlinehawksapprentice
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Re: A 'lot' of Karo questions! [Re: liveby]
    #3526559 - 12/21/04 04:49 AM (12 years, 8 months ago)

1st. Huh??? Haven't seen it with karo.

2nd. Excellent way, if you can get an antibiotic like gentamycin into that agar.

3rd. Love karo, have had nothing but great things with it.


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"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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Offlinedjred
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Re: A 'lot' of Karo questions! [Re: hawksapprentice]
    #3527508 - 12/21/04 01:32 PM (12 years, 8 months ago)

karo is the bomb..i have almost 100% half pints in a week,well 1 jar is almost 100% but i keep my jars very warm cambodia seems to like the extra warm tit


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InvisibleKlingonFromUranus
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Re: A 'lot' of Karo questions! [Re: djred]
    #3527581 - 12/21/04 02:04 PM (12 years, 8 months ago)

IMHO karo is only really useful if you have a very small amount of spores or liquid inoculum and want to increase it so that many jars/bags can be inoculated with a small amount of original spores. Karo does increase colonization times, but only by a few days which is not worth it IMHO unless you need to knock up huge amounts of grain and need a fast turnover. The regular small time home grower can do just fine with spores.


--------------------


Edited by KlingonFromUranus (12/21/04 02:04 PM)


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OfflineMajestic
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Re: A 'lot' of Karo questions! [Re: hawksapprentice]
    #3528287 - 12/21/04 05:20 PM (12 years, 8 months ago)

I dont have time right now, late for work, but tonite if i have the energy ill upload a few pics of the clumped spores...I swirled it vigorously and now they do not pull apart. I wish i had a pic from before to show the difference, but best i can say is first day or two they were just floating, roughly evenly distributed in the water, many settled on the bottom. Now theres one gigantic mass of purple spores, and one small one with minimal spores floating around. ~D


--------------------
"The Oracle at Delphi is quite possibly real...
I watched a documentary on it on Discovery channel."  -Darcho

People will always be tempted to wipe their feet,
on anything with 'welcome' written on it.

:regularshroom:?:regularshroom: "mushroom, what?"
_\ -/_


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OfflineMajestic
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Re: A 'lot' of Karo questions! [Re: Majestic]
    #3530290 - 12/22/04 03:32 AM (12 years, 8 months ago)

Some pics

This is the clumping at the bottom, as of sometime today, the two masses have conjoined, and it took a lot of swirling to get 3 pieces and a few straggling spores to seperate:



From below:



Proof I'm doin this right =\:



Solution was 2 tsp of karo in 200 ml purified water...mixed very very well...this was inoculated on the 15th or 16th...

~D thanks for your patience and help!


--------------------
"The Oracle at Delphi is quite possibly real...
I watched a documentary on it on Discovery channel."  -Darcho

People will always be tempted to wipe their feet,
on anything with 'welcome' written on it.

:regularshroom:?:regularshroom: "mushroom, what?"
_\ -/_


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Invisiblemuse_sick
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Re: A 'lot' of Karo questions! [Re: Majestic]
    #3530306 - 12/22/04 03:38 AM (12 years, 8 months ago)

2 tablespooons or 2 teaspoons?
7 days is long for no colonization to be seen, but not overly long
I'm assuming that that pic tells me they are @ 82 F
see if you can knock temp up a degree or two
sometimes that makes a difference
how many CCs of spores did you inject?
also, it doesn't really matter in regards to your question, but is that a pint jar or a 1/2 pint jar?
i've found that 1/2 pints are better for karo,
sometimes with pint jars it's hard to get the syringe in there all the way when you start to run low


--------------------
:beakedwhale:


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OfflineMajestic
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Re: A 'lot' of Karo questions! [Re: muse_sick]
    #3530324 - 12/22/04 03:50 AM (12 years, 8 months ago)

teaspoons.
ya temp goes from 82-83, water temp is 89, i guess better insulation might raise it a few degrees.
2 ccs of very heavy solution.
pint jar.

=\ ~D


--------------------
"The Oracle at Delphi is quite possibly real...
I watched a documentary on it on Discovery channel."  -Darcho

People will always be tempted to wipe their feet,
on anything with 'welcome' written on it.

:regularshroom:?:regularshroom: "mushroom, what?"
_\ -/_


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Invisiblemuse_sick
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Registered: 12/15/03
Posts: 9,399
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Re: A 'lot' of Karo questions! [Re: Majestic]
    #3530330 - 12/22/04 03:53 AM (12 years, 8 months ago)

well sounds like everything is alright
i usually go a bit heavier on karo (1tablespoon/100ml h2o)
but i'll bet your colonization is just delayed b/c of the increased volume of karo/h2o solution
give it a few days then check back in
if you have any questions or want some feedback feel free to pm me


--------------------
:beakedwhale:


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OfflineMajestic
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Re: A 'lot' of Karo questions! [Re: muse_sick]
    #3606154 - 01/10/05 02:05 AM (12 years, 8 months ago)

Well, the jar above decided to finally do something. About 3 days ago, possibly 4 I noticed lil fluffs of white showing up, now its bout the size of a nickle.



I really never expected this to do anything, but I kept it in my incubator just 'cause' =P. All in all this took a rough min total of 21 days to germinate. I used 200ml of water with 2 teaspoons of karo. I'm not really sure how the spores found each other and formed a clump, perhaps from me shaking. In conclusion I believe its better to do as small volume of water as you can, it seems a few germ and bonded the mass then the rest finally took off 3 weeks later.

~D


--------------------
"The Oracle at Delphi is quite possibly real...
I watched a documentary on it on Discovery channel."  -Darcho

People will always be tempted to wipe their feet,
on anything with 'welcome' written on it.

:regularshroom:?:regularshroom: "mushroom, what?"
_\ -/_


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop: ½ Pint Jars, Agar

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