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Amazon Shop for: ½ Pint Jars, Agar, Paul Stamets, Petri Dish, Potato Dextrose

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InvisibleThe One
Mr Shroom
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The Spore Works Standard Agar Culturing Kit
    #3515179 - 12/17/04 11:12 PM (11 years, 11 months ago)

Hi all.  Has anyone bought the Standard Agar Culturing Kit from "The Spore Works" what?s it like.  I have ordered it and it should be here in a week or so.  But what I want to know if anyone here is has used it, and how did they get on with it.  I've always done PF teks and used a syringe that I have made my self.  I want to learn this agar technique and was wondering if anyone can give me some help.  I've read the teks on the shroomery but am still not sure on exactly some of the things.

This is what I think so far.

I make a solution of the agar and some water sterilize it in the PC and them pour it in Petri dishes, and let it solidify.  Once that happens I inject the solid form with spores and kepp it in a dark place.  I then wait for the solid agar to colonize and then cut a piece out and use that to colonize my PF jars.

What I'm not sure on is, what do I cover the Petri dishes with so they don't get contaminated will cling film do?

Do I let the whole agar solid mix colonize, or just part of it be colonized before I cut a piece out?

How could I make a syringe mix with this colonized agar mix. 

Thanks for the help. :cool:


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Offlineliveby
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Re: The Spore Works Standard Agar Culturing Kit [Re: The One]
    #3515376 - 12/18/04 12:08 AM (11 years, 11 months ago)

bump


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Invisibletripndicular
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Re: The Spore Works Standard Agar Culturing Kit [Re: The One]
    #3515453 - 12/18/04 12:27 AM (11 years, 11 months ago)

Is the agar in powder form , or is it premade(gooy tan jelly looking stuff) ?

Did kit not come with basic instructions on prepping the agar , pouring it ?

Petris should have come with a lid and a bottom , normally sold in "sleeves" of 20-25 "whole" dishes .

You have some serious research to do before you even think of opening that package up when it hits your door . If you do not you will have wasted materials , money , time . :thumbdown:

Hey pick up a copy of TMC  :thumbup: It is wise investment IMHO  :cool:

Good Reading... and Luck!


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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InvisibleThe One
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Re: The Spore Works Standard Agar Culturing Kit [Re: tripndicular]
    #3517084 - 12/18/04 02:20 PM (11 years, 11 months ago)

here is a list of what is on it's way

1 bottle 8oz net Potato Dextrose Agar
3 Sleeves (20) of 100mm x 15mm Sterile Plastic Petri Dishes
1 500ml Nalgene Erlenmeyer Flask
1 Alcohol Lamp with wick
1 Surgical Steel Scalpel with 4 disposable blades

There is no mention of a guide coming, on how to make the agar tek. But on the spore works site in "Lab Notes" there is a little tutorial, but is isn't that clear.


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Edited by The One (12/18/04 02:22 PM)


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Amazon Shop for: ½ Pint Jars, Agar, Paul Stamets, Petri Dish, Potato Dextrose

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