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InvisiblePinback
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Re: Breaking up clumps of spores in syringe [Re: agar]
    #3475617 - 12/10/04 10:59 AM (20 years, 1 month ago)

Found this paper about ultrasonical preparation of mycelial suspensions when I searched this topic:

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Invisiblethemills
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Registered: 07/24/04
Posts: 177
Re: Breaking up clumps of spores in syringe [Re: Pinback]
    #3482487 - 12/11/04 04:33 PM (20 years, 1 month ago)

So pretty much with the mycotopia research and that paper (also on mycotopia) you don't have to "doubt" it works, it does, and some say better.
Duh Duh (Law & Order sound)


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There are 2 possible outcomes: If the result confirms the hypothesis, then you've made a measurement. If the result is contrary to the hypothesis, then you've made a discovery.
-Enrico Fermi
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InvisibleHippie3
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Re: Breaking up clumps of spores in syringe [Re: themills]
    #3485274 - 12/12/04 04:54 AM (20 years, 1 month ago)

Quote:

themills said:
So pretty much with the mycotopia research and that paper (also on mycotopia) you don't have to "doubt" it works, it does, and some say better.
Duh Duh (Law & Order sound)




yes, indeed.
it works
and well at that.
and with sonicators
going for $20-30 on ebay
i think lots of folks
could benefit from this tek.


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Mycotopia

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Offlinehyphae
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
    #3485798 - 12/12/04 11:01 AM (20 years, 1 month ago)

$20-30 is a sweet price and well within my range, I bet the wife would also love this for cleaning her rings and things! :wink: I really can't see this so much for spore dispersal so much but if this breaks up myc so well say in the jar this would indeed be an invaluable tool. I love toys :wink: :laugh:


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Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
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"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

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OfflineMushroomFriend
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Re: Breaking up clumps of spores in syringe [Re: hyphae]
    #3485860 - 12/12/04 11:14 AM (20 years, 1 month ago)

What is Jet dry used for, Im trying to find a local equivalent then....
It is no soap is it?


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OfflineAnnoA
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Re: Breaking up clumps of spores in syringe [Re: MushroomFriend]
    #3486043 - 12/12/04 12:09 PM (20 years, 1 month ago)

Dishwasher rinsing liquid.

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OfflineMushroomFriend
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Re: Breaking up clumps of spores in syringe [Re: Anno]
    #3486414 - 12/12/04 01:42 PM (20 years, 1 month ago)

Hmmz never use that, the supermarket might have it though.
Thanks!


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InvisibleHippie3
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Re: Breaking up clumps of spores in syringe [Re: hyphae]
    #3490146 - 12/13/04 08:02 AM (20 years, 1 month ago)

"if this breaks up myc so well say in the jar this would indeed be an invaluable tool."

yep.
spores or tissue
matters not to the sonicator.
same principles in play.
just tissue is more fragile
so less time is needed.


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Invisiblemycofile
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
    #3491841 - 12/13/04 02:53 PM (20 years, 1 month ago)

Hey hip, I've been wrong before!  :wink:
this tek looks good to me.  I wonder if liquid innocs might benefit from being cultured continuously in a sonic bath?  Just leave a jar in the cleaner and leave it on for a few days...Anybody experimenting on that?


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"From a certain point of view"
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I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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InvisibleHippie3
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Re: Breaking up clumps of spores in syringe [Re: mycofile]
    #3496049 - 12/14/04 05:39 AM (20 years, 1 month ago)

Quote:

mycofile said:
Hey hip, I've been wrong before!  :wink:
this tek looks good to me.  I wonder if liquid innocs might benefit from being cultured continuously in a sonic bath?  Just leave a jar in the cleaner and leave it on for a few days...Anybody experimenting on that?




hmm, not yet
but we'll get on it now.
your thinking is it would
speed growth by creating more
points yes ?


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Mycotopia

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InvisibleHippie3
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Re: Breaking up clumps of spores in syringe [Re: mycofile]
    #3496059 - 12/14/04 05:42 AM (20 years, 1 month ago)

some new pix of sonicator in action
on a old dry print





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Invisiblemycofile
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
    #3498036 - 12/14/04 03:30 PM (20 years, 1 month ago)

My thinking is that it would create more points, yes, by breaking any clumps of spores up constantly. But also that the constant mixing might be helpful in some way. Most people just shake their jars every day or so, but lets remember that the pros use stirplates to constantly agitate their liquids. Perhaps these sonicators might offer some benefit as yet unknown?


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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InvisibleHippie3
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Re: Breaking up clumps of spores in syringe [Re: mycofile]
    #3502583 - 12/15/04 06:11 AM (20 years, 1 month ago)

i see where you're going.
might have merit.
but i'd imagine like most things
there's a limit ,
perhaps a 2-3 second pulse once a day
might be a good place to start testing.
we can increase it from there if results are positive.
i'll get our 'topiates to work on some new experiments
asap.
thx for the input.
:thumbup:


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Offlinesinfull7
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
    #3507409 - 12/16/04 01:48 AM (20 years, 1 month ago)

there is also a Syringe needle That B&D makes .it filters particles down to .5 microns its the brown precision glides 1 1/2 in 18GA needles they sell them at almost every place that Ive got the regular pink needles. i use these to draw water into the syringe then just add a pink regular tip to draw up the spores. do you use a lot of spores in a typical syringe. because i only scrape the inoc loop on it twice. so mine are not that dark at all it just seems to help a lot . in the fact that i don't get a 100 sub-strains on one plate (just lets me see what i wanna grab a Lil better) . I've got black syringes from some vendors and even after diluting them 5 or more times its still dark . also i don't get any clumping using this ( its a spore dilution tech ) stamets method. am i alone in this because Ive noticed as of late vendors offering super DARK prints .

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Invisibleagar
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Re: Breaking up clumps of spores in syringe [Re: sinfull7]
    #3507506 - 12/16/04 03:55 AM (20 years, 1 month ago)

I prefer an almost clear syringe.

My rule of thumb is, if on CLOSE inspection I can see a few..... THERE ARE PLENTY IN IT.

Frankly, a dark syringe is a waste of spores. All you need is enough to get the job done :thumbup:.

Enough to get the job done  :eek:1000 times over :crazy2: in a single syringe, just doesnt make sense :confused:. At least to frugal agar. :stoned:

As an example, this WBS jar contains one 0.5 CC squirt from an almost clear syringe.


This jar contains 1 CC in 2 squirts


This print contains about a million ++ spores.


If you are frugal, this one print could produce 100 VIABLE syringes.


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Invisibleohmatic
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Re: Breaking up clumps of spores in syringe [Re: agar]
    #3507517 - 12/16/04 04:11 AM (20 years, 1 month ago)

actually i must be honest, i didnt read the whole thread
but heres a good way to break up clumps:

simply dismantle ur syringe and throw in a lil glasshed,
put it back together and autoclave.

once u drew up the syringe, prior to use u simply shake it around a bit
and the glasshed will do its job to mix up the solution quite properly.

u shouldnt worry too much about it plunding the needle,
since u simply hold it at an angle and all is good.

simple and works every time.
peace ohm :mushroom2:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

Edited by ohmatic (12/16/04 04:28 AM)

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Invisibleagar
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Re: Breaking up clumps of spores in syringe [Re: ohmatic]
    #3507524 - 12/16/04 04:15 AM (20 years, 1 month ago)

A stainless steel BB works, to.


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InvisibleHippie3
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Re: Breaking up clumps of spores in syringe [Re: agar]
    #3507661 - 12/16/04 06:35 AM (20 years, 1 month ago)

the point isn't to make dark syringes,
i'll agree.
the ones in the pix above are far darker than needed,
it's just an example.
one could easily use much more water
and dilute the concentration 10 fold
and still get stellar success.

one advantage of the sonication method
over adding a bb, etc.
is that the sonic tek
eliminates one more vector thru
which contams might get in.
no need to add anything,
no bb, no jet dry,
nadda.
just water and spores.
dilute to the desired concentration.
as simple and as effective
as one can possibly get.


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Mycotopia

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Invisibleohmatic
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Re: Breaking up clumps of spores in syringe [Re: Hippie3]
    #3508644 - 12/16/04 12:27 PM (20 years, 1 month ago)

Quote:

Hippie3 said:
one advantage of the sonication method
over adding a bb, etc.
is that the sonic tek
eliminates one more vector thru
which contams might get in.




well u tell me how contams are gonna get on a glass shed i place
into a empty syringe prior to autoklaving please...
once its toasted, there's not a single contam left,
or do u know some contams withstanding a whopping 20 psi over 90 mins?
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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InvisiblePrisoner#1
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Re: Breaking up clumps of spores in syringe [Re: ohmatic]
    #3515005 - 12/17/04 07:42 PM (20 years, 1 month ago)

there are quite a few spores that can survive a 'killing' heat, some wont
germinate until that heat has been reached, there are also seeds
that will only germinate after they have been burned, these high temp
spores are something that many of us will never have to worry about
but they do exist

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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Original Sensible Seeds Autoflowering Cannabis Seeds   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Liquid Cultures


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