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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Brown Rice Flour, Vermiculite, Wild Bird Seed

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OfflineMushroomFriend
Vargen ska fanleva!!
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Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 3 hours
Syringe tek (may already exist)
    #3497623 - 12/14/04 04:03 PM (11 years, 11 months ago)

I wanna share my way of syringe making with you.

I need:
-50 CC jar with lid (autoclavable)
-Sharp knife / small l33t scissors
-Alcohol
-Sterile Syringe
- pins (to put in mushcap)

I did as follows:
- sterilized jar and lid in the oven for 30 mins att 200F
- let cool
- wipe pin with alcohol paper
- put pin in mushcap
- cut stem
- put cap in jar real quick (take it by the pin)
- close with lid immediatly

This is where I am now

Tomorrow:
- after 23 hours I boil some water
- Sterile syringe (oven or PC) I suck 10 CC (0r bigger up to 40 or 50) water in it. (I want a FAT syringe if you know what I mean)
- let cool an hour
- (loosen lid) wipe needle with alcohol
- take of lid
- take away mushcap
- squirt the sterile water in jar
- put lid back
- shake the hell out of it
- loosen lid,
- suck your syringe full with sporewater!! :thumbup:

RESULTING IN THIS:


This might best be done in glovebox or flowhood, I do it in the oven or in open air.


Any comments?


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Edited by MushroomFriend (12/15/04 11:18 AM)


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OfflineMajestic
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Registered: 11/06/04
Posts: 196
Loc: Florence, Oregon
Last seen: 2 years, 10 months
Re: Syringe tek (may already exist) [Re: MushroomFriend]
    #3497691 - 12/14/04 04:16 PM (11 years, 11 months ago)

I like it =) Infact after my first flush I'll prob do that. Maybe even use a pyrex dish for multiple caps? thanks for sharing.  :grin: ~D


--------------------
"The Oracle at Delphi is quite possibly real...
I watched a documentary on it on Discovery channel."  -Darcho

People will always be tempted to wipe their feet,
on anything with 'welcome' written on it.

:regularshroom:?:regularshroom: "mushroom, what?"
_\ -/_


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Offlinefrock
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Registered: 02/22/04
Posts: 262
Last seen: 5 years, 10 months
Re: Syringe tek (may already exist) [Re: MushroomFriend]
    #3497699 - 12/14/04 04:17 PM (11 years, 11 months ago)

I do the same thing pretty much, except I do not sterilize my the jar in an oven or anything. I just wash it and wipe it out with alcohol and then let the alcohol evaporate.

I also, have been reusing the same 6 syringes for the last 1/2 a year, and I just boil some water, suck some up, squirt in sink, and repeat about 4 - 5 times for each syringe.


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OfflineIGnosticAbhorI
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Registered: 11/06/04
Posts: 4,898
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Re: Syringe tek (may already exist) [Re: frock]
    #3497740 - 12/14/04 04:27 PM (11 years, 11 months ago)

^^ same here. I use the oven tek tho. The hot rising air keeps contams. away so that when i take off the lid i dont have to wry. nice tek tho and yea, i think its alread y a tek,lol but nice thinking!


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OfflineMushroomFriend
Vargen ska fanleva!!
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Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 3 hours
Re: Syringe tek (may already exist) [Re: frock]
    #3497764 - 12/14/04 04:34 PM (11 years, 11 months ago)

Cool that you like it, a part I got from IRc from a chap but I forget his name.

I am making some ordinary sporeprints as well (tryin to).

ONe could use tek above, but then with a coffeefilter (put in oven a bit to sterilise it) between lid and jar so the moist from teh cap and spores can go there.

But I dont wanna waste space so I:
- prefold (&unfold) foil
- alcohol wipe it
- lay on clean towel
- put sterile drinking glass on it (i do multiple prints so i have a alcohol wiped ovenbowl over it)
- alcohol wipe pin and put in mushcap
- cut the cap with clean knife / scissors
- lift glas /oven bowl and put cap on foil
- put glas back
- wait 24 hours > CURRENTLY WAITING :P
- sneak the cap away from underneath teh glas
- take glas away and fold the foil! :thumbup:

A part is from Shirley Knots Tek.

Comments on this?

I am glad to experiment with all this! :laugh: I like my new hobby and its superb to have my friends grow in my own house & energy! :thumbup:

Bye,
MF


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Offlineatmosphere
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Registered: 11/01/04
Posts: 64
Loc: Eugene
Last seen: 11 years, 10 months
Re: Syringe tek (may already exist) [Re: MushroomFriend]
    #3498153 - 12/14/04 05:49 PM (11 years, 11 months ago)

nice lookin cake man...I'm jealous. Is that your first grow?
Whats the strain and substrate?


--------------------
F.O.T. ..o0o.. Groovin like a Tuvan


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OfflineMushroomFriend
Vargen ska fanleva!!
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Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 3 hours
Re: Syringe tek (may already exist) [Re: atmosphere]
    #3498249 - 12/14/04 06:03 PM (11 years, 11 months ago)

Hey atmosphere!

Yes my first fruit cake! :laugh: I had some with other fungi before.. :grin:

It grew so fast, pins after 3 days (last friday) and yesterday evening the biggest ones started opening their caps, so today I first took the 3 biggest for sporeprinting and syringemaking. They are not on the pic too bad huh!?

It is PF classic strain and I used 3,5-1-1 (verm-water-brf). But the fine verm gave it a hard time during colonisation, about 6 weeks!! Maybe thats why it pinned so fast too.

Also I dunked (bout 12 hours) and rolled. Its being dunked again now, tomoorow Ill roll him and he goes for his second flush.

Actually the 3,5-1-1 ratio was a mistake, I have new cakes in incubator where I use 2-1-1. GT strain and after 3 days first mycelium in all of them and in a pint of WBS.

They fruited between 68 and 78 (night day) and i fanned and sprayed manual but sunday I put my airpump in the wet geolite....

:thumbup:

MF


--------------------


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OfflineMushroomFriend
Vargen ska fanleva!!
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Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 3 hours
Re: Syringe tek (may already exist) [Re: MushroomFriend]
    #3502901 - 12/15/04 11:19 AM (11 years, 11 months ago)

Updated 1st post with pic of my first Syringe! :wink:

Deep purple ;p


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InvisibleTODAY
Battletoad
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Registered: 09/25/03
Posts: 10,218
Loc: Metropolis City, USA
Re: Syringe tek (may already exist) [Re: MushroomFriend]
    #3504440 - 12/15/04 06:00 PM (11 years, 11 months ago)

cool mang.


--------------------

ca'rouse (k-rouz)
intr.v.
To engage in boisterous, drunken merrymaking.


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OfflineMushroomFriend
Vargen ska fanleva!!
Male User Gallery

Registered: 10/12/04
Posts: 4,055
Loc: The Druid Peak Pack.
Last seen: 1 year, 3 hours
Re: Syringe tek (may already exist) [Re: TODAY]
    #3504498 - 12/15/04 06:06 PM (11 years, 11 months ago)

mannggggg ? :P

Hey you still got that fake rating there? They deleted mine! :laugh:


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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Brown Rice Flour, Vermiculite, Wild Bird Seed

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