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Registered: 08/18/04
Posts: 126
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Problems with needle and inoculation?
    #3478433 - 12/10/04 09:34 PM (13 years, 4 months ago)

OK, I got these syringes (from TLG) and was knocking up some WBS and BRF jars tonight. I noticed when I took the cap off the syringes that the needles were very large bore compared to the syringes I had previously. I didn't think much of it and knocked up my WBS without incident. I started knocking up the BRF jars and immediately started having probs with the needle getting verm stuck in it to the point I could not get anything to come out. I repeatedly had to keep pulling it back out and clearing it. At one point I thought I had it all out and went to flame the needle and I don't know what happened exactly but it let off this loud pop (louder than they normally crackle a bit) and shot off a piece of something and when I looked at the end of the needle it was actually BENT, no more sharp tip. The only thing I could figure to do was to take the needle off the previous syringe and swap them, flaming and alcohol everything. I am very concerned about about my sterility and also I wasted a lot of spore water trying to get the needles unclogged. Is there a trick to this I don't know or something. I never had this trouble with the smaller bore syringes.

Anyone who says size doesn't matter has been seeing too many small shrooms!

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Registered: 11/19/04
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Re: Problems with needle and inoculation? [Re: Katrina]
    #3478488 - 12/10/04 09:47 PM (13 years, 4 months ago)

yeah, moisture, in this case verm, will "pop" when heated.
I always pull the plunger back a bit and push on, it unclogs.
Can you sterilize and transfer the solution to a smaller ga. syringe?

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Re: Problems with needle and inoculation? [Re: Katrina]
    #3478563 - 12/10/04 09:59 PM (13 years, 4 months ago)

when you insert the needle into the jar, try running the cut end against the
side of the jar, that should eliminate the clogs

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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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