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 NeonBlack
enthusiast
Registered: 11/24/00
Posts: 331
Last seen: 13 years, 1 month
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 Brown Rice Syrup Agar 
#347836 - 06/24/01 07:00 PM (21 years, 3 months ago) |
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I was walking through my local health food store and came across some brown rice syrup.. I looked around here and found little mention of it so I decided to give it a try..
My mix: 20 g agar (food grade), 4 tsp brown rice syrup, a spoonful or two of powdered rye, and a liter of water.. I mixed and pressure cooked for 45 mins.. Today I innoculated five of the poured plates with EQ spores.. Sounds like good agar to me.. We'll see how it goes..
Edited by NeonBlack on 06/24/01 12:26 PM.
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egghead
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Registered: 04/25/01
Posts: 1,054
Loc: Milky Way
Last seen: 20 years, 6 months
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 Re: Brown Rice Syrup Agar [Re: NeonBlack]
#347838 - 06/24/01 07:07 PM (21 years, 3 months ago) |
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Good for growth and germination. Not for long-term storage, though. Did you check the pH of the final solution? That seems like a lot of syrup for 1 L of water.
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Where there's skill, there's a better way..
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NeonBlack
enthusiast
Registered: 11/24/00
Posts: 331
Last seen: 13 years, 1 month
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Re: Brown Rice Syrup Agar [Re: egghead]
#347845 - 06/24/01 07:25 PM (21 years, 3 months ago) |
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Yeah.. I poured these plates so I could weed out contams from a suspect print.. I won't be storing any of these.. I made a rough estimate as to the amount of syrup to use by comparing it to the ratio that honey is used.. But on second thought it does sound like a little bit much.. I do think that I used a teaspoon more than I could have gotten away with.. We'll just see how it goes.. If anything it would just slow growth though.. I'll update when I see some germination.
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NeonBlack
enthusiast
Registered: 11/24/00
Posts: 331
Last seen: 13 years, 1 month
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Re: Brown Rice Syrup Agar [Re: NeonBlack]
#349906 - 06/27/01 06:14 PM (21 years, 3 months ago) |
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Ok.. It's been two days and this morning I saw germination on all five plates.. Looks good so far.. One question, maybe I missed this or something but I don't know.. Is it not standard practice to incubate agar plates upside down to keep condensation from raining down on the agar? When I looked at my plates I saw that in all cases the mycelium was growing towards the lid of the plate and not along the surface of the agar.. I have been storing them upside down up until now when I flipped them over.. So, what's the general consensus? Incubate agar plates upside down or not?
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FungusFlipper
newbie
Registered: 05/26/01
Posts: 28
Last seen: 21 years, 1 month
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Re: Brown Rice Syrup Agar [Re: NeonBlack]
#350315 - 06/28/01 03:58 AM (21 years, 3 months ago) |
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You don't need to incubate upside down. Just store plates that have been poured but not innoc'd.
FF
Why Jack, these are magic beans!
Support the FSR! Ralph needs your help!
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Mycotopia: your shroom away from shroom!
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--------------------
Why Jack, these are magic beans!
Support the FSR! Ralph needs your help!
http://go.to/FreeSporeRing
Mycotopia: your shroom away from shroom!
http://myctopia.yage.net
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