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OfflineNeonBlack
enthusiast
Registered: 11/24/00
Posts: 331
Last seen: 13 years, 1 month
Brown Rice Syrup Agar
    #347836 - 06/24/01 07:00 PM (21 years, 3 months ago)

I was walking through my local health food store and came across some brown rice syrup.. I looked around here and found little mention of it so I decided to give it a try..
My mix: 20 g agar (food grade), 4 tsp brown rice syrup, a spoonful or two of powdered rye, and a liter of water.. I mixed and pressure cooked for 45 mins.. Today I innoculated five of the poured plates with EQ spores.. Sounds like good agar to me.. We'll see how it goes..

Edited by NeonBlack on 06/24/01 12:26 PM.



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Offlineegghead
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Registered: 04/25/01
Posts: 1,054
Loc: Milky Way
Last seen: 20 years, 6 months
Re: Brown Rice Syrup Agar [Re: NeonBlack]
    #347838 - 06/24/01 07:07 PM (21 years, 3 months ago)

Good for growth and germination. Not for long-term storage, though. Did you check the pH of the final solution? That seems like a lot of syrup for 1 L of water.



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OfflineNeonBlack
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Registered: 11/24/00
Posts: 331
Last seen: 13 years, 1 month
Re: Brown Rice Syrup Agar [Re: egghead]
    #347845 - 06/24/01 07:25 PM (21 years, 3 months ago)

Yeah.. I poured these plates so I could weed out contams from a suspect print.. I won't be storing any of these.. I made a rough estimate as to the amount of syrup to use by comparing it to the ratio that honey is used.. But on second thought it does sound like a little bit much.. I do think that I used a teaspoon more than I could have gotten away with.. We'll just see how it goes.. If anything it would just slow growth though.. I'll update when I see some germination.



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OfflineNeonBlack
enthusiast
Registered: 11/24/00
Posts: 331
Last seen: 13 years, 1 month
Re: Brown Rice Syrup Agar [Re: NeonBlack]
    #349906 - 06/27/01 06:14 PM (21 years, 3 months ago)

Ok.. It's been two days and this morning I saw germination on all five plates.. Looks good so far.. One question, maybe I missed this or something but I don't know.. Is it not standard practice to incubate agar plates upside down to keep condensation from raining down on the agar? When I looked at my plates I saw that in all cases the mycelium was growing towards the lid of the plate and not along the surface of the agar.. I have been storing them upside down up until now when I flipped them over.. So, what's the general consensus? Incubate agar plates upside down or not?



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OfflineFungusFlipper
newbie
Registered: 05/26/01
Posts: 28
Last seen: 21 years, 1 month
Re: Brown Rice Syrup Agar [Re: NeonBlack]
    #350315 - 06/28/01 03:58 AM (21 years, 3 months ago)

You don't need to incubate upside down. Just store plates that have been poured but not innoc'd.

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--------------------
Why Jack, these are magic beans!
Support the FSR! Ralph needs your help!
http://go.to/FreeSporeRing
Mycotopia: your shroom away from shroom!
http://myctopia.yage.net


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