Home | Community | Message Board


RVF Garden Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Paul Stamets

Jump to first unread post. Pages: 1
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Odd temperature dependent behavior in some Ps. cubenis strains
    #3477719 - 12/10/04 07:28 PM (12 years, 4 hours ago)

Has anyone else observe seemingly anomalous behavior in some Ps. cubensis strains?

In several cases (in this case several different B+/GT substrains) mycelium was shaken a few days after inoculation. The millet jars were being incubated at approx. 80 degrees Fahrenheit. After shaking the growth seemed to have stopped, but upon being put in a lower temperature environment (approx. 65-70 F) growth just took off.

This has happened with several different grows and the spawn was, in some cases, used to inoculate bulk substrate with 0% contamination and good yields. B.E. was around 100%.

One hypothesis for this was bacterial contamination, but even so the fact that growth revitalized to about the same linear growth rate as other jars being incubated at 80 F seems puzzling.

Does anyone have any ideas what else might cause this? In most cases the jars were from the same isolate also.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleJoshua
Holoman
Male

Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: Odd temperature dependent behavior in some Ps. cubenis strains [Re: Alkaloids]
    #3477976 - 12/10/04 08:05 PM (12 years, 4 hours ago)

My first thought is bacterial contamination. My second is possible uneven heating of your incubation chamber.

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"


Post Extras: Print Post  Remind Me! Notify Moderator
Invisible@cro
new name
 User Gallery

Registered: 12/07/02
Posts: 1,224
Loc: The PNW
Re: Odd temperature dependent behavior in some Ps. cubenis strains [Re: Alkaloids]
    #3478019 - 12/10/04 08:13 PM (12 years, 4 hours ago)

Sometimes it can take mycelium time to recover. Maybe you are witnessing this but because you are also changing the temps at the point where it has recovered, thinking the change in temps is the cause?


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Re: Odd temperature dependent behavior in some Ps. cubenis strains [Re: Joshua]
    #3481735 - 12/11/04 03:05 PM (11 years, 11 months ago)

The incubator has neglible temperature fluctuations so that can safely be ruled out. What is a bit more perplexing is that some jars have had samples (a few kernels of millet) taken out and plated with no signs of bacterial contamination. Some jars have had bacteria, but plowed right through it and had no problems even after transfer to bulk substrates.

Thanks for the feedback.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleAlkaloids
3,4,5-trimethoxyphenethylamine
Male

Registered: 11/16/98
Posts: 743
Loc: pubis mons
Re: Odd temperature dependent behavior in some Ps. cubenis strains [Re: @cro]
    #3481761 - 12/11/04 03:13 PM (11 years, 11 months ago)

  Stamets has pointed out that bacterially contaminated spawn can be placed at lower temperatures, but with lower yields (40-60% according to him).  In this case B.E. still ending up being 100% or a bit higher. It should be noted that some of these isolates were yielding upwards of 200% B.E. when everything was optimal though.
  Recovery was apparently completely stalled until the jars were put into a colder environment.  Meaning that they showed neglible signs of regrowth for up to several weeks, but then, once put out to pasture, recovered within a day or two.

  Thanks ..... more information to think about.  :3rd_eye:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Temperatures of Psilocybin degeneration? whyIdied 2,004 12 05/18/01 06:17 PM
by jonnyshaggs420
* All woodlovers are Ps.Cyanescens?? Zen Peddler 1,817 12 01/16/03 11:47 PM
by Zen Peddler
* MAJOR BREAKTHROUGH - FASTER PINNING VIA TEMPERATURE CYCLING
( 1 2 3 all )
Hannibal88 10,911 40 07/05/13 05:22 PM
by RogerRabbit
* Growing ps. pseudocyanea Phyl 688 7 09/11/03 08:36 PM
by pluteus
* ANNO:Ps. bohemica Azure 1,271 4 04/28/03 04:49 PM
by Effed
* non - cubenis species on vermiculite cakes? goldtop 1,254 10 10/08/02 07:43 PM
by Zen Peddler
* cubenis metabolites. doze 1,370 18 02/15/09 03:32 PM
by ShadowSpore
* Ps. semilanceata cultivation? Have spores, need info BambiEar 3,014 13 08/04/04 12:08 PM
by ambros

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
731 topic views. 0 members, 3 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
FreeSpores.com
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.001 seconds on 14 queries.