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lemon_lw
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IV
#3476691 - 12/10/04 02:38 PM (19 years, 3 months ago) |
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has anyone used NS or lactated ringers to hydrate cakes? if so how was it? or should i just not do this? i have a couple bags laying around, any thoughts? i was thinking bout just putting a catheter in the middle with a slow drip to rehydrate while fruiting.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
Edited by lemon_lw (12/10/04 02:41 PM)
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scatmanrav
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I highly doubt if its been tried the person in the world who tried it will come around the board while this post is here..sounds like a very uniquie idea and probably the only way your gonna know is by trying it.
I am inclinded to believe however that unless its about 5 drops a day it would be to much. Mushrooms use alot of water but not THAT much..
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lemon_lw
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i could close it off and then once a day give it a couple of drops ill have to rig up one of my fruiting chambers to do this. i think ill wait untill i get back from leave and experiment with this. ill definatly keep you guys filled in if its anygood. anybody else?
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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Fungi_x
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You may want to look into "Straw in cake" if you have not herd about it. You take a drinking straw and stick it into the center of the cake and fill it with water and the cake will take what it needs.
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lemon_lw
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on the same token do you think it would only take the liquid it needs from the catheter? and still what positive or negative effects will these solutions have on them?
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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scatmanrav
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It could contaminate fairly easy as well. H202 in a clear bag would break down from the light quickly..or in water with any things in it. The h202 would only last a few days..iodine perhaps wouldnt break down and could be used..
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lemon_lw
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but the inside of an IV bag is already sterile.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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Fungi_x
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"on the same token do you think it would only take the liquid it needs from the catheter?"
I would guess it would.
Just found were I got the info from check it out.
http://www.earthstongue.com/site/388422/page/63257
has some info. If the stuff in the bag is sterile you may be on to something. Your still prolly raising the risk of contams but if you have strong mycelia they can fight it.
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scatmanrav
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Can you add distilled water absolutly sterily? And are you sure nothing can go up the needle through the tube? Anything but sterile distilled water could contain contams. I know little about IV bags so I'm just making sure you think of all problems that may exist..thats about all I can think of though so I'll leave it for others thoughts..
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Fungi_x
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One thing you must remember is that a fully colonized cake is VERY contam resistant.
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Prisoner#1
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Quote:
lemon_lw said: on the same token do you think it would only take the liquid it needs from the catheter? and still what positive or negative effects will these solutions have on them?
it will run through the cake, I tried this with a syringe with no plunger, in less than a day the full 10cc syringe was empty.
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lemon_lw
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good shit fungi. but yeah it would be safe to say all my equipment is steril. everything is unopened. the only one with a possibility would be the tubing cause its not sealed the best caus ethey use crappy bags to store. but the bag and needle i can guarantee. i can always do a flush on the tubing with some alcohol followed by h2o + h2o2. that would get it sterile. but still what about the solution of normal saline and lactated ringers? somebody enlighten me please.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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scatmanrav
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Quote:
Fungi_x said: One thing you must remember is that a fully colonized cake is VERY contam resistant.
True but if contams get in a bag of water and breed you'll have a liquid culture of mold pumping into your cake..and it COULD add contamination risk..
Seems it might just be easiest to fill a syringe and just inject a half cc a day or so and remove the syringe..
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lemon_lw
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well a contam couldnt get back into the bag because gravity just wont allow it. it would have to travel through 10 feet of tubing fighting gravity the whole way. so there would be no other way into the bag. bu tyeah a syringe could work, but you up your odds by reintorducing the needle and syringe everyday where as the iv would be semiperminant. thanks fo rthe info pris, but i can make it so it aonly gets like 1cc a day. i can throw a 20g needle in there to lower the drip rate a bit. and if i can get my hands on a smaller guage i will. but still the question about the solutions in the bag (sorry for repeating this but i really want to know)?
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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scatmanrav
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You could leave the needle in and unscrew the syringe..Eitherway if kept sterile you shouldnt have a problem taking the need in and out. Check out ryvmed.com for smaller needles..I think they have some smaller like 23g.
Whats the question solutions in the bag? Should only have distilled water in the bag..I didnt see another question perhaps thats why its being missed..
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lemon_lw
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its iv bags of normal saline and lactated ringers, i was wondering how they would effect moushroom growth. ill just look around at work and find some smaller needles.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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ryan
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Not a good idea. You wouldnt add salt to your cakes would you? then why use normal saline or lactated ringers.
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lemon_lw
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Re: IV [Re: ryan]
#3477512 - 12/10/04 04:41 PM (19 years, 3 months ago) |
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ok good now we are getting somewhere. would you add salt? would this fuck up the ph? i dont know if you can.and ryan who were you before you were ryan? you say member since 1997.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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Katrina
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Normal saline bags as well as Lactated Ringers can contain dextrose. Lactated ringers also normally contains potassium, chloride, calcium, and sodium and is used because it closely resembles the electrolyte composition of human blood. I have no clue, what it might do to cakes, but just thought I tell what was in it in case you didn't know.
-------------------- Anyone who says size doesn't matter has been seeing too many small shrooms!
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lemon_lw
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are you really sure dextrose is in NS? cause it doesnt say so on the bag and it should not be in there considering the effects it could have on a diabetic patient. the only ingredients should be sodium and chloride. yes lactated ringers has it. some smart ass words were once writen hear but i took them away cause i realized the info she gave can benifit anyone else who reads this post.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
Edited by lemon_lw (12/10/04 05:38 PM)
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