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OfflinesporesourceV
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Registered: 01/13/04
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Last seen: 10 years, 20 days
Ling Chi mold
    #3460281 - 12/07/04 05:03 PM (11 years, 11 months ago)

I am working on my technique before offering edibles, and medicinals.
I was curious what others do about contamination on culture plates, other than what is posted on the board. I have Ling Chi growing on MYA without h2o2 and intentionally infected a spot with trych to practice removal operations. I used a scalpel to cut out the trych and then flooded the entire plate with h2o2. The trych did not reappear, and the Ling Chi went dorment for a few days, and then went charging back it filled the rest of the plate in less than a week, but the initial part of Ling Chi turned a light tan color. anybody else have a good way to destroy mold without killing the mushroom?


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Offlineshirley knott
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Re: Ling Chi mold [Re: sporesource]
    #3461009 - 12/07/04 07:54 PM (11 years, 11 months ago)

transplant good areas away to fresh plates, rather than trying to cut out bad areas, they say.


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buh


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OfflinesporesourceV
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Re: Ling Chi mold [Re: shirley knott]
    #3461609 - 12/07/04 10:14 PM (11 years, 11 months ago)

Yes that is the normal process, and I understand traditional process, and transfer including running mycelia to escape other contams at this point. I am looking to see if anyone is innovating.


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Offlinefalcon
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Re: Ling Chi bacteria contams [Re: sporesource]
    #3493032 - 12/13/04 07:48 PM (11 years, 11 months ago)

I just tried something I had seen a year ago
that seems to work so far. If you think you
have a bacterial contamination, that doesn't
show on the plate. A bacterial contam. that
slows the growth or stops the growth
of the mycellium before it completely
colonizes the plate.

Take a small wedge and put it in a sterile
jar. Take a jar of sterile used tea leaves
and pour some on the wedge, a couple of
inches. Close up the jar and let the
mycellium run through the tea leaves.
When it gets to the surface take
some thing sterile to transfer
the mycellium back onto agar.

Why its supposed to work: the mycellium
pushes throught the tea leaves and
leaves the bacteria behind.

Wet tea leaves are hard to pour.
It was sort of messy.
It would be much better to do
this with a glass tube or
plastic tube with removeable
lids at either end.
One lid should be vented.
Sterilize the tea in the tube.
Put the wedge in at one end
and retrieve at the other end.

I think I read this tek on mushworld.com.
I may not have I can't find the link for it.
I may have read it here.


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Offlineragadinks
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Re: Ling Chi bacteria contams [Re: falcon]
    #3495431 - 12/14/04 02:50 AM (11 years, 11 months ago)

Sounds really interesting - maybe I am going to run a test when I have the next contaminated plates.
Does the sort of the tea mater ?


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Offlinefalcon
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Re: Ling Chi bacteria contams [Re: ragadinks]
    #3497970 - 12/14/04 05:20 PM (11 years, 11 months ago)

I use orange and black pekoe mixes.
I save the used tea in the freezer,
until I have enough to use.

I think green tea would work for the
antibactial thing too.
I used green tea as a supplement once
with sawdust and the mycellium liked
it.


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InvisibleATWAR
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Registered: 01/26/03
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Loc: #108768 in line...
Re: Ling Chi bacteria contams [Re: falcon]
    #3502111 - 12/15/04 04:49 AM (11 years, 11 months ago)

A friend of mine here (charvo) once bleached a contaminated plate before he threw it out (I believe he soaked it with full strength household bleach). Somehow it escaped the trash can tek, and was forgotten. To his surprise, the mycelium recovered and continued to grow right over the dead contamination...


I have not tried this personally however.


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OfflineFood
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Re: Ling Chi bacteria contams [Re: ATWAR]
    #3526637 - 12/21/04 05:55 AM (11 years, 11 months ago)

I love the tea leaves idea . Cant explain why in words but ooh its nice .

Oh man and that mycelium living through the bleach -  :tongue: woah !  :eek:


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--------mushworld.com-----More info than you can throw a stick at-


Edited by Food (12/21/04 05:58 AM)


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InvisibleATWAR
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Re: Ling Chi bacteria contams [Re: Food]
    #3526674 - 12/21/04 06:58 AM (11 years, 11 months ago)

I agree, this method deserves a try.


I have heard of similar methods of growing mycelia through a non-nutritive substrate to isolate from mold (such as with water-agar, or sawdust in a glass tube). I think if I ever need to resort to something this extreme, I may give it a try... I may just bleach an old plate and see what happens too  :wink:  The bleach agar TEK....  :tongue:

I think I have read somewhere that raising the Ph of the agar can help, but I have never tried raising it above 8.0. I don't have much trouble with isolation from molds except when cloning wild mushrooms, or bad spores. The mold usually always wins in these cases as the mycelia is slow to grow initially. In the case of black pin mold, I was able to place the culture (trametes versicolor) in my window in direct sunlight. The turkey tail mycelium continued to colonize, while the mold stopped and phsically died. I was able to transfer clean tissue easily. Green cultures are always thrown away, so my mold isolation experience is limited in that arena...


The best thing you can do to increase your odds of success, is increase the number isolation attempts.


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Offlinefalcon
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Re: Ling Chi bacteria contams [Re: ATWAR]
    #3528496 - 12/21/04 06:38 PM (11 years, 11 months ago)

I throw away all moldy cultures too.
Anything on agar or grain is
pressure cooked before I open the jars.

Transferring away from mold :thumbup:
very cool.


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Invisiblebooknik
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Registered: 12/11/04
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Re: Ling Chi bacteria contams [Re: ATWAR]
    #3732396 - 02/04/05 10:28 PM (11 years, 9 months ago)

ATWAR said:
>I was able to place the culture (Trametes versicolor) in my window in direct sunlight. The turkey tail mycelium continued to colonize, while the mold stopped and phsically died.

To what would you attribute the demise of the mold? UV light?


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InvisibleATWAR
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Re: Ling Chi bacteria contams [Re: sporesource]
    #3735695 - 02/05/05 05:04 PM (11 years, 9 months ago)

I can only speculate that it was either the spectrum, or the intensity of the light. The mold (a black pin mold) stopped growing or died (I did not watch it for a few days), and was consumed by the mycelium. Approximately a dime size colony completely covered the wedge and surrounding agar. when I saw this I placed it into my window for the heck of it to see if it would work (I think I have read of this). I had other plates so I was not concerned about this particular one.



It did indeed work and I wish I had documented it.
I have not tried it with any other type molds (or cultures).
IME, it would have overrun that plate in 2 days max...


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