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Offlinenu2mycology
trying out pooh
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Registered: 09/25/04
Posts: 136
Loc: Florida USA
Last seen: 10 years, 7 days
inactive liquid culture????
    #3459178 - 12/07/04 10:42 AM (19 years, 4 months ago)

A few weeks ago I made some karo jars. I incubated them for 2 or 3 weeks then innocculated some jars of rye with them. After more than a week there is NO signs of life in ANY of the innocculated jars!
  Could it be possible that my karo jars didn't work for some reason?
  I could see the swirl of growth in it before I sucked anything out to innocculate with.
Might just try and innocculate them all again.
By the way, I am using an 86 degree TIT :confused: :mushroom2: :rasta:


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The author of this lies more than the government and you should not believe anything he writes!

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Offlinelemon_lw
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Registered: 10/17/04
Posts: 3,622
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Re: inactive liquid culture???? [Re: nu2mycology]
    #3459188 - 12/07/04 10:45 AM (19 years, 4 months ago)

did you shake the karo up?


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz

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OfflineZeroArmy27
I didn't go to work for a month.

Registered: 08/31/04
Posts: 1,169
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Re: inactive liquid culture???? [Re: lemon_lw]
    #3459197 - 12/07/04 10:48 AM (19 years, 4 months ago)

also, you should have had some white fuzzy growth in there. my karo grew into a cotton ball looking growth... little white flakes on the bottom too. what'd yours look like?

should give it the shakedown before you innoc with it.


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"a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut

"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker

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Offlinenu2mycology
trying out pooh
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Registered: 09/25/04
Posts: 136
Loc: Florida USA
Last seen: 10 years, 7 days
Re: inactive liquid culture???? [Re: ZeroArmy27]
    #3459203 - 12/07/04 10:50 AM (19 years, 4 months ago)

before I shook mine it looked like a big blob of phlegm at the bottom of the jar...


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The author of this lies more than the government and you should not believe anything he writes!

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OfflineZeroArmy27
I didn't go to work for a month.

Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
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Re: inactive liquid culture???? [Re: nu2mycology]
    #3459211 - 12/07/04 10:51 AM (19 years, 4 months ago)

yep, sounds about right.

was it bright white?


--------------------
"a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut

"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker

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Offlinedjred
newbie

Registered: 08/27/04
Posts: 922
Last seen: 15 years, 8 months
Re: inactive liquid culture???? [Re: ZeroArmy27]
    #3459305 - 12/07/04 11:23 AM (19 years, 4 months ago)

my karo jars have that cotton look also you have to squirt shake qhat ever to break it up..other wise won't be able to suck any ion the syringe. my myc is a little clear but yes its white.

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OfflineZeroArmy27
I didn't go to work for a month.

Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 1 month
Re: inactive liquid culture???? [Re: djred]
    #3459362 - 12/07/04 11:38 AM (19 years, 4 months ago)

what kind of needles are you using also? smaller needles can keep the mycelium from being sucked up into the syringe.


--------------------
"a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut

"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker

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Offlinedjred
newbie

Registered: 08/27/04
Posts: 922
Last seen: 15 years, 8 months
Re: inactive liquid culture???? [Re: ZeroArmy27]
    #3459416 - 12/07/04 11:57 AM (19 years, 4 months ago)

12 cc from tlg they are standard it will suck up just i ahd to break up the myc like a mother f#$%^ so it would go throught but ye si did get it i could see myc in the tube

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