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Amazon Shop: ½ Pint Jars, Agar

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Offlinepornaga
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Think I forgot to shake up the syringe...
    #3456378 - 12/06/04 08:42 PM (12 years, 6 months ago)

Yeah, like the title says, I think I forgot to shake up the syringe before I inoculated. Basically, all I need to know is if this is going to present any real problems.

I guess I'll know for certain in about 4 days. I figure if I don't see any mycellium growth by then, I think I'll open a new syringe and inject the duds. Your thoughts, please.


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Invisibleagar
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Re: Think I forgot to shake up the syringe... [Re: pornaga]
    #3456403 - 12/06/04 08:47 PM (12 years, 6 months ago)

If the syringe contained viable spores, you squirted them into the jars didnt you. Whatever the case (without shaking a syringe), some should have made it into each jar. Some more than other & those take off faster.


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Offline13eetleJuice
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Re: Think I forgot to shake up the syringe... [Re: agar]
    #3456407 - 12/06/04 08:49 PM (12 years, 6 months ago)

And 4 days really isn't enough time to tell anything. It can take that long for the spores to germinate. I'd wait 7-10 days at least.


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Offlinelemon_lw
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Re: Think I forgot to shake up the syringe... [Re: agar]
    #3456408 - 12/06/04 08:49 PM (12 years, 6 months ago)

wait longer than 4 days. wait 2 weeks or you could be wasting good solution. and agar is right except for some crazy case.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Invisibleagar
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Re: Think I forgot to shake up the syringe... [Re: lemon_lw]
    #3456428 - 12/06/04 08:53 PM (12 years, 6 months ago)

Quote:

lemon_lw said: and agar is right except for some crazy case.




:confused:.................

What crazy case?


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InvisiblePrisoner#1
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Re: Think I forgot to shake up the syringe... [Re: agar]
    #3456433 - 12/06/04 08:55 PM (12 years, 6 months ago)

Quote:

agar said:
Quote:

lemon_lw said: and agar is right except for some crazy case.




:confused:.................

What crazy case?




I think he's refering to me.... :crazy2:


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Offlinelemon_lw
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Re: Think I forgot to shake up the syringe... [Re: Prisoner#1]
    #3456448 - 12/06/04 08:58 PM (12 years, 6 months ago)

yeah its about pris.1. no i was just talking, if by some MIRACULOUS case that no spores got in. but the odds are so crazy its unbelievable. sorry to confuse you agar.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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Offlinehyphae
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Re: Think I forgot to shake up the syringe... [Re: lemon_lw]
    #3456538 - 12/06/04 09:25 PM (12 years, 6 months ago)

Shaking is important as you all know, I'm sure some of you also realize spores clump and they have a tendency to be attracted the the damn plunger nice thing is it doesn't take much movement to move this clump and even breakup the spore clumps slightly so as agar said some almost always make it into the jars but also remember nothing is guaranteed in this world but death and taxes and Da Prez is a dumbshit!


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Offlinelesstutrey
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Re: Think I forgot to shake up the syringe... [Re: hyphae]
    #3456640 - 12/06/04 09:44 PM (12 years, 6 months ago)

heyo. just to let you know. someone inoc'ed 5 jars.. forgot to shake.. only the first jar showed life :o so depending on how many spores are in the syringe it can make a huge difference.


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Amazon Shop: ½ Pint Jars, Agar

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