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Offlinepainloc21
Dumb Ass
Registered: 11/12/04
Posts: 81
Last seen: 11 years, 2 months
cold spore syringe
    #3447795 - 12/04/04 09:45 PM (19 years, 3 months ago)

Ive done my soak, simmer, pc of mu quart jars of WBS and i am waiting on them to cool overnight. My question is should my spore syringe be room temp or could i just take it out of the fridge when im ready and use it? Also what else should i do to the syringe before using it? Should i shake it right before use? thank you

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Offlinelemon_lw
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Registered: 10/17/04
Posts: 3,622
Loc: That Way
Last seen: 16 years, 6 months
Re: cold spore syringe [Re: painloc21]
    #3447828 - 12/04/04 09:52 PM (19 years, 3 months ago)

it doesnt matter bout it being cold. shake it up real real real good. then flame your needle. let needle cool and inoc. spray lysol if you dont have a glove box or a relative sterile environment.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz

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InvisibleSuper_Blunt
Candyman
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Registered: 10/25/04
Posts: 3,140
Re: cold spore syringe [Re: lemon_lw]
    #3447833 - 12/04/04 09:54 PM (19 years, 3 months ago)

:syringe:


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Offlinepainloc21
Dumb Ass
Registered: 11/12/04
Posts: 81
Last seen: 11 years, 2 months
Re: cold spore syringe [Re: lemon_lw]
    #3447849 - 12/04/04 09:58 PM (19 years, 3 months ago)

great. thanks for the fast response. I have another question i forgot to ask in my first post so i will ask now. I had no tyvek so i used 5 cofee filters. then the metal lid with polly fill then 2 peices of foil. Should this be o,k or am i asking for contams?

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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: cold spore syringe [Re: painloc21]
    #3447879 - 12/04/04 10:04 PM (19 years, 3 months ago)

Polyfil will work. Tyvek is 1000 times better, IMHO

Coffee filters are about 15 or 20 micron, plus if wet, they can wick in contams, onto the polyfil, then into a jar. So, you may be SOL, only time will tell.

Get Tyvek asap, for future use.

Not much help, sorry.


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Offlinelemon_lw
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Registered: 10/17/04
Posts: 3,622
Loc: That Way
Last seen: 16 years, 6 months
Re: cold spore syringe [Re: agar]
    #3447914 - 12/04/04 10:12 PM (19 years, 3 months ago)

yeah i used coffee filters at first. i just sprayed the shit out of them with lysol before puting on my jars. but yeah you sound good, try to upgrade to tyvek soon though.


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz

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Offlinepainloc21
Dumb Ass
Registered: 11/12/04
Posts: 81
Last seen: 11 years, 2 months
Re: cold spore syringe [Re: lemon_lw]
    #3447935 - 12/04/04 10:17 PM (19 years, 3 months ago)

Damn and i shook the jars up after i took them out of the pc so they might have got wet. well at least i have plenty of WBS and spores so if it dont work i can try again. i will make sure to use tyvek next time! thanks guys

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Offlinelesstutrey
All Weather Associate
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Registered: 10/24/04
Posts: 495
Loc: Chicagoland
Last seen: 9 years, 6 months
Re: cold spore syringe [Re: painloc21]
    #3447962 - 12/04/04 10:22 PM (19 years, 3 months ago)

tyvek is free in the US, no reason not to use it.

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Offlinepainloc21
Dumb Ass
Registered: 11/12/04
Posts: 81
Last seen: 11 years, 2 months
Re: cold spore syringe [Re: lesstutrey]
    #3447981 - 12/04/04 10:27 PM (19 years, 3 months ago)

yeah i know i orderd some from the postal service for free. I was just impatient and it probably screwed me. lol. but i bought alot of WBS and spores and such so i should be o.k. Its all learning to me.

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OfflineIGnosticAbhorI
Stranger-er

Registered: 11/06/04
Posts: 4,899
Last seen: 10 months, 3 days
Re: cold spore syringe [Re: painloc21]
    #3448035 - 12/04/04 10:42 PM (19 years, 3 months ago)

Hey pain you said you got tyvek for free from ordering it, what site did you go to? Thankx

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Offlinepainloc21
Dumb Ass
Registered: 11/12/04
Posts: 81
Last seen: 11 years, 2 months
Re: cold spore syringe [Re: IGnosticAbhorI]
    #3449303 - 12/05/04 09:25 AM (19 years, 3 months ago)

http://shop.usps.com/cgi-bin/vsbv/postal...mp;appName=SSOL

There you go. free tyvek. I found the link here on this site in a old post.

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