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OfflineIfIWereARichMan
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Explain to me the joys of substrain isolation (karo jars etc)
    #3442366 - 12/03/04 03:34 PM (12 years, 2 days ago)

Everyone talks about using agar or karo jars to isolate a single strain with which you can inoculate your substrate to so that you will get better yields, right?

I dont have my copy of TMC here at college with me, but I recall reading that a multispore inoculated petri dish will end up with a single dominant substrain that "eats up" all the other mycelium substrains. If thats the case, then any multispore inoculation will result in a substrain isolated fruiting. It just wont be one thats proven to be a fantastic fruiting strain, although I doubt most people here go to the trouble of keeping slants to use after fruiting ability has been tested.

Even if thats not the case, and several different substrains will germinate from a multispore, then wouldnt a karo jar end up with several different substrains? Its not like a petri dish where you can see the sectoring. Its just a giant puffball. Whats the deal?


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Offlinediscman1
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: IfIWereARichMan]
    #3442386 - 12/03/04 03:42 PM (12 years, 2 days ago)

Yup.

My karo jars are multispore.


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Invisiblederx
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: discman1]
    #3442486 - 12/03/04 04:20 PM (12 years, 2 days ago)

you cant isolate a strain via karo tek. Liquid culture is not sectoring in any form. When you are sectoring you try to take 1 piece of mycelium that is the substrain you want, then you grow it out on a new dish, then pull from that again if it's not isolated. It's not just a 1 step process in which you inoculate a petri dish and one strain takes over, that is incorrect. Got it?


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.


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OfflineIfIWereARichMan
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: derx]
    #3446345 - 12/04/04 06:16 PM (12 years, 1 day ago)

Then karo shouldnt give any better yields than innoculations straight from a spore syringe, just better colonizing times. Right?


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Invisiblederx
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: IfIWereARichMan]
    #3447507 - 12/04/04 10:33 PM (12 years, 22 hours ago)

thats exactly what they're used for, cutting 4-6 days off colonizing time.


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.


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OfflineIfIWereARichMan
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: derx]
    #3449900 - 12/05/04 02:01 PM (12 years, 7 hours ago)

All the teks/postings I've read make them out to be the second coming of christ, and i could never understand why. Thanks


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OfflineShdwstr
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: derx]
    #3449986 - 12/05/04 02:23 PM (12 years, 7 hours ago)

Quote:

derx said:
you cant isolate a strain via karo tek. Liquid culture is not sectoring in any form. When you are sectoring you try to take 1 piece of mycelium that is the substrain you want, then you grow it out on a new dish, then pull from that again if it's not isolated. It's not just a 1 step process in which you inoculate a petri dish and one strain takes over, that is incorrect. Got it?




If you start with spores in either agar,karo or grain... it is still multispore. Advantage in karo... faster colonization of your spawn. (but you still have to wait for the karo to colonize)
If you start with a piece of a fruitbody its cloning. Your Karo is now a liquid culture, not liquid mycelium. Advantage, faster colonization of spawn and more uniform (clone) fruits.


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Invisibleambros
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Re: [Re: Shdwstr]
    #3450210 - 12/05/04 03:35 PM (12 years, 5 hours ago)

can you let your liquid cultures incubating under light?


Edited by ambros (12/05/04 04:11 PM)


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OfflineShdwstr
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: ambros]
    #3450222 - 12/05/04 03:41 PM (12 years, 5 hours ago)

I'm not entirely sure of the question... or the answer to it.
Rule of thumb... follow standard incubation methods, warm, dark, etc.


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InvisibleKoala Koolio
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: Shdwstr]
    #3451158 - 12/05/04 08:30 PM (12 years, 57 minutes ago)

Yes you speed it up


--------------------
You're not like the others. You like the same things I do. Wax paper, boiled football leather... dog breath. We're not hitch-hiking anymore, we're riding!


Edited by elgr (02/04/06 01:44 AM)


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Invisiblederx
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: Shdwstr]
    #3451528 - 12/05/04 09:45 PM (11 years, 11 months ago)

(-)


--------------------
better living through chemistry

OVERGROW the government!!

it's not a war on drugs, it's a war on personal freedom, ok, thats what it is.


Edited by derx (12/05/04 11:36 PM)


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OfflineShdwstr
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: derx]
    #3452039 - 12/05/04 11:33 PM (11 years, 11 months ago)

I was simply elaborating on your answer.
No disrepect intended.
Carry on  :smirk:


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OfflineShdwstr
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: Koala Koolio]
    #3452096 - 12/05/04 11:44 PM (11 years, 11 months ago)

Quote:

elgr said:
Its silly that the other advantage of karo (even with multi spore) hasn't been mentioned. Yes you speed it up, but you increase the amount of syringes you can have by a hell of a lot.




:laugh:  Your right... that was my reason to do it too!


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InvisibleEonTan
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: IfIWereARichMan]
    #3454421 - 12/06/04 01:44 PM (11 years, 11 months ago)

The joys of substrain isloation is BEING ABLE TO VIEW the VARIETY that is held within a population of a Strain in a controlled way.

Karo is just liquid culture. The advantages were mentioned: Fast colonization and increased inocula.

If you start with spores it is multispore. Many substrains will result, and more then one will typically be present at all phases of growth.

If you use agar and isolate a single strain, either by culturing sectors, or by waiting to clone a fruit that comes from the multispore plate, you can start a single substrain liquid culture. Now you add another advantage of inoculuation with a single substrain.
More uniformity in growth at all phases, even more so if the clone was taken from a known producer in your exact environmnetal parameters.


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Offlinerberg79
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Re: Explain to me the joys of substrain isolation (karo jars etc) [Re: IfIWereARichMan]
    #12263845 - 03/24/10 06:09 PM (6 years, 8 months ago)

How do you know that the dominant strain will be one capable of fruiting? Is a dominant specimen of mycelium statistically likely to fruit, and/or a fruiting mycelium statistically likely to be a dominant colonizer?

One of the problems with MSIs (as i understand from reading on shroomery) is that you'll likely get many offspring that are not genetically sound and will not fruit, which is why you could potentially expect to see uneven pinsets or cakes that pin only in a couple spots.


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&T


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